[Effect of exogenous paclobutrazol on the drought resistance of seedlings under drought stress].

To clarify the response mechanism of exogenous paclobutrazol on drought resistance in seedlings, we investigated the effects of spraying different concentrations of paclobutrazol (25, 50, 100 mg·L) on the photosynthetic and antioxidant systems of 2-year-old seedlings under drought stress using natural drought method. The results showed that drought stress significantly reduced the photosynthesis and broke the dynamic balance of antioxidant system in seedlings. Spraying with different concentrations of paclobutrazol effectively alleviated the negative impacts of drought stress, and enhanced the defense capability of photosynthetic and antioxidant systems, with the 100 mg·L paclobutrazol treatment being the most effective.

A Case of False Insulin Test Results Due to the Megaloblastic Anemia.

Background: Megaloblastic anemia is a subtype of anemia with increased red blood cell volume. These megaloblastic cells can be easily destroyed in the bone marrow and spleen, leading to ineffective hematopoiesis. Insulin-degrading enzymes (IDE) in erythrocytes can break down the insulin into amino acid fragments; thus, when hemolysis occurs, IDE can be released into the blood, resulting in low insulin measurement values.

Pseudo-Elevation of Anti Double Stranded DNA IgG Antibody Caused by Rheumatoid Factor.

Background: The examination of anti-double stranded DNA (ds-DNA) IgG antibody is of great significance for the diagnosis, differential diagnosis, assessment of disease activity, and prognosis of disease recurrence in SLE.

Methods: We used a chemiluminescence method to detect ds-DNA IgG and found that the levels of ds-DNA IgG antibody in the patient's serum were significantly increased and the indirect immunofluorescence (IIF) test result was negative. Laboratory tests show that the patient's RF level far exceeds the upper limit of their reference range.

Knowledge, attitudes, and practices of gouty arthritis in the general population aged > 30.

Background: A knowledge of gouty arthritis could help in the primary prevention of the disease development and lead to an early diagnosis if it occurs. This study investigated the knowledge, attitudes, and practices (KAP) toward gouty arthritis in the general population > 30 years old.

Methods: This web-based cross-sectional study was conducted among the general population > 30 years old between January and March 2023 in Chengdu, Sichuan.

Pseudo Elevation of TSH and ACTH Caused by Heterophilic Antibodies: a Case Report and Literature Review.

Background: TSH and ACTH are crucial hormones for diagnosing thyroid and adrenal diseases, and incorrect test reports can cause significant harm to patients.

Methods: The TSH and ACTH levels on the testing system of our laboratory were measured using "sandwich" assays. The patient had heterophilic antibodies in their body, causing a false increase in TSH and ACTH levels.

A Pseudo Elevation of CEA Caused by Epidemic Hemorrhagic Fever.

Background: Heterophilic antibodies (HA) are one of the main substances that interfere with immunology, especially chemiluminescence immunoassay. Non-specific binding, labeling antibodies, bridging to capture antibodies, or labeling antigens can interfere with the detection process, leading to serious discrepancies between the measured results and clinical manifestations, and even delaying clinical diagnosis and treatment.

Methods: This paper is a case of epidemic hemorrhagic fever causing pseudo CEA elevation caused by heterophagy induced antibodies in the body.

Effects of species mixing on maximum size-density relationships in Chinese fir ( (Lamb.) Hook.)-dominated mixed forests converted from even-aged pure stands.

Introduction: Density management is a key silvicultural tool in management programs that enhances compositional and structural diversity and hence forest growth during the conversion of even-aged pure stands into mixed forests.

Methods: To determine the optimum stand density, a model of maximum size-density relationships was employed to explore the relationship of the self-thinning trajectory with growth, species mixing, latitude, and site conditions during the transition of even-aged pure Chinese fir stands to Chinese fir-dominated mixed forests using stochastic frontier analysis. Data were obtained from a total of 591 permanent plots located in Fujian, Jiangxi, Zhejiang, and Anhui provinces in southern China.

Colonization of root endophytic fungus improves drought tolerance of seedlings by regulating metabolome and proteome.

is an important forest tree species for plantations because of its rapid growth and high yield of oleoresins. Although plantations distribute in warm and wet southern China, drought, sometime serious and long time, often occurs in the region. To explore drought tolerance of and usage of beneficial microorganisms, seedlings were planted in pots and were inoculated with root endophytic fungus and finally were treated with drought stress for 53 d.

Genome-Wide Identification, Characterization, and Expression Analysis of the Family Genes under Abiotic Stresses in .

The BRI1 EMS suppressor 1(BES1) transcription factor is a crucial regulator in the signaling pathway of Brassinosteroid (BR) and plays an important role in plant growth and response to abiotic stress. Although the identification and functional validation of genes have been extensively explored in various plant species, the understanding of their role in woody plants-particularly the endangered species (Hemsl.) Yang-remains limited.

Infection with multi‑drug resistant organisms in patients with limb fractures: Analysis of risk factors and pathogens.

Infection with multi-drug resistant organisms (MDROs) has emerged as a global problem in medical institutions. Overuse of antibiotics is the main cause of drug resistance. Notably, the incidence of infection with MDROs increases in patients with limb fractures who have undergone invasive surgery.

Research progress on productivity of mixed forest.

One of the effective ways to improve the productivity of mixed forests is enhancing resource use efficiency based on the biological characteristics of afforestation tree species. Resource use efficiency is affected by tree species interactions and environmental conditions through applying appropriate cultivation patterns. In this study, we evaluated two estimating methods for the productivity of mixed forest, analyzed the internal mechanism of interspecific tree competition and complementary effects on mixed forest productivity, clarified external factors of growth space and habitat factors control over productivity of mixed forest, discussed the effects of tree species composition, stand density and site quality on productivity, and illustrated the productivity trajectory during the development of mixed forests.

Metagenomics reveals the effects of glyphosate on soil microbial communities and functional profiles of C and P cycling in the competitive vegetation control process of Chinese fir plantation.

Although considerable efforts have been devoted to investigate the behavior of glyphosate on microbiome in various environment, knowledge about the soil microbial community and functional profile in weeds control process of the Chinese fir plantation are limited. In this study, shotgun metagenomic sequencing was used to determine the abundance and diversity of microbial communities and functional genes after foliar application of glyphosate for 1, 2, 3 and 4 months in a Chinese fir plantation. The results showed that glyphosate increased the copy numbers (qPCR) of 16S rRNA gene for 16.

TAGAP activates Th17 cell differentiation by promoting RhoA and NLRP3 to accelerate rheumatoid arthritis development.

Rheumatoid arthritis (RA) is a chronic autoimmune disorder that can give rise to joint swelling and inflammation, potentially affecting the entire body, closely linked to the state of T cells. The T-cell activation Rho GTPase activating protein (TAGAP) is associated with many autoimmune diseases including RA and is directly linked to the differentiation of Th17 cells. The present study intends to investigate the influence of TAGAP on the RA progression and its mechanism to empower new treatments for RA.

Study on the effects of stand density management of Chinese fir plantation in Northern China.

The aim of this study was to clarify the mechanism by which thinning alters stand structure and affects forest productivity by characterizing changes in stand quantitative maturity age, stand diameter distribution, structural heterogeneity, and forest productivity of Chinese fir plantations at different thinning times and intensities. Our findings provide insights into how the density of stands could be modified to enhance the yield and timber quality of Chinese fir plantations. The significance of differences in individual tree volume, stand volume, and timber merchantable volume was determined using one-way analysis of variance and Duncan tests.

Targeted and untargeted metabolomics reveals deep analysis of drought stress responses in needles and roots of seedlings.

Drought stress is one of major environmental stresses affecting plant growth and yield. Although trees are planted in rainy southern China, local drought sometime occurs and can last several months, further affecting their growth and resin production. In this study, seedlings were treated with long-term drought (42 d), and then targeted and untargeted metabolomics analysis were carried out to evaluate drought tolerance of .

Novel insights on genes and pathways involved in Pinus elliottii response to resinosis.

Pinus elliottii, an important coniferous timber species, has recently become one of the most popular sources of resin in China. Resinosis is a common disease that may negatively affect pine tree growth and production. In this study, we used single-molecule real-time sequencing and Illumina RNA sequencing to generate an accurate transcriptome for P.

The Application Value of SMI Technology and Contrast-Enhanced Ultrasound in the Differential Diagnosis of Benign and Malignant Thyroid Nodules.

Thyroid disease has always been a common and frequent disease in clinical medicine, and its disease detection rate has been increasing year by year. Thyroid diseases are mainly divided into two categories: thyroid diseases treated by medical treatment and thyroid diseases treated by surgery. Thyroid cancer has also become one of the most common malignant secretory tumor diseases today.

The mechanisms and prediction of non-structural carbohydrates accretion and depletion after mechanical wounding in slash pine (Pinus elliottii) using near-infrared reflectance spectroscopy.

Background: The allocation of non-structural carbohydrates (NSCs) plays a critical role in the physiology and metabolism of tree growth and survival defense. However, little is known about the allocation of NSC after continuous mechanical wounding of pine by resin tapping during tree growth.

Results: Here, we examine the NSC allocation in plant tissues after 3 year lasting resin tapping, and also investigate the use of near-infrared reflectance (NIR) spectroscopy to quantify the NSC, starch and free sugar (e.

Variation in Growth, Wood Density, and Stem Taper Along the Stem in Self-Thinning Stands of .

Silvicultural practices greatly improve the economic value of wood products from forests. Stem dimensions, wood density, and stem form are closely linked to end-product performance. This research aimed to examine the effects of stand density and stem height on variables that reflect ring growth and wood properties of stands during the self-thinning phase.

Prediction and Comparisons of Turpentine Content in Slash Pine at Different Slope Positions Using Near-Infrared Spectroscopy.

Pine resin is one of the best known and most exploited non-wood products. Resin is a complex mixture of terpenes produced by specialized cells that are dedicated to tree defense. Chemical defenses are plastic properties, and concentrations of chemical defenses can be adjusted based on environmental factors, such as resource availability.

Spectrometric Prediction of Nitrogen Content in Different Tissues of Slash Pine Trees.

The internal cycling of nitrogen (N) storage and consumption in trees is an important physiological mechanism associated with tree growth. Here, we examined the capability of near-infrared spectroscopy (NIR) to quantify the concentration across tissue types (needle, trunk, branch, and root) without time and cost-consuming. The NIR spectral data of different tissues from slash pine trees were collected, and the concentration in each tissue was determined using standard analytical method in laboratory.

IRF2 inhibits ZIKV replication by promoting FAM111A expression to enhance the host restriction effect of RFC3.

Background: Although interferon regulatory factor 2 (IRF2) was reported to stimulate virus replication by suppressing the type I interferon signaling pathway, because cell cycle arrest was found to promote viral replication, IRF2-regulated replication fork factor (FAM111A and RFC3) might be able to affect ZIKV replication. In this study, we aimed to investigate the function of IRF2, FAM111A and RFC3 to ZIKV replication and underlying mechanism.

Methods: siIRF2, siFAM111A, siRFC3 and pIRF2 in ZIKV-infected A549, 2FTGH and U5A cells were used to explore the mechanism of IRF2 to inhibit ZIKV replication.

Comparative Analyses of Phyllosphere Bacterial Communities and Metabolomes in Newly Developed Needles of (Lamb.) Hook. at Four Stages of Stand Growth.

Host-plant-associated bacteria affect the growth, vigor, and nutrient availability of the host plant. However, phyllosphere bacteria have received less research attention and their functions remain elusive, especially in forest ecosystems. In this study, we collected newly developed needles from sapling (age 5 years), juvenile (15 years), mature (25 years), and overmature (35 years) stands of Chinese fir [ (Lamb.

Myxovirus resistance protein A activates type I IFN signaling pathway to inhibit Zika virus replication.

Myxovirus resistance protein A(MxA), one of the dynamin superfamily of large guanosine triphosphatase and a classical interferon stimulated gene (ISG) induced by type I interferons (IFNs), plays antiviral role in various virus infections. However, the effect of MxA on Zika virus (ZIKV) infection and its underlying mechanism remain elusive. In this study, we aimed to explore the role of MxA in ZIKV infection and its potential mechanisms.