Novel Broad-Spectrum Aptamer Used to Build Fe-Co Nanoporous Carbon Highly Sensitive Sensing Platform for the Detection of Multiple Organophosphorus Pesticides.

In this work, broad-spectrum aptamers for organophosphorus pesticides (OPs) were obtained by alternate target systematic evolution of ligands by exponential enrichment screening. The secondary and tertiary structure analyses of the aptamer inferred that the neck-loop structure formed a G-triplex structure with the target. In addition, optimization of the sheared aptamer resulted in a stronger affinity ( = 86.

Dual-ratiometric aptasensor for simultaneous detection of malathion and profenofos based on hairpin tetrahedral DNA nanostructures.

Due to the diversification and complexity of organophosphorus pesticide residues brings great challenges to the detection work. Therefore, we developed a dual-ratiometric electrochemical aptasensor that could detect malathion (MAL) and profenofos (PRO) simultaneously. In this study, metal ions, hairpin-tetrahedral DNA nanostructures (HP-TDN) and nanocomposites were used as signal tracers, sensing framework and signal amplification strategy respectively to develop the aptasensor.