Publications by authors named "Hongfeng Hao"
Objective: To observe the pathologic effects of marrow hematopoietic cell with immunologic injury induced by infection immunity activation in patients with immune related hematocytopenia syndrome (IRHS) and elucidate its immunologic mechanism and clinical significance.
Methods: A total of 276 IRHS patients with acute and chronic infections were recruited from 2008 to 2013. ELISA was used to detect the levels of interleukin-6 (IL-6), IL-12, IL-17 and interferon gamma (IFN-γ) in peripheral blood sera of IRHS patients before and after therapy.
The immune injury effects and clinical significance of eosinophils in auto-immune-related hematocytopenia.
J Immunoassay Immunochem
September 2015
Eosinophils (EOS) quantity, active state, peroxidase activity (POX), and HLA-DR expression in bone marrow of 176 Auto-Immune-Related Hematocytopenia (AIRH) patients were analyzed. Immunofluorescent staining (IF) is performed to observe the expression of immunizing molecules on EOS. In serum of AIRH patients the levels of IL-4, IL-5, IL-6, IL-12, IL-17, and IFN- γ were increased but there was no significance on IL-2 level.
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- The study aims to understand how eosinophilic granulocytes (EOS) affect anti-leukemic responses in chronic myelogenous leukemia (CML) patients.
- Elevated serum levels of IL-12 and IL-17 were observed in CML patients, especially in those with activated EOS, suggesting a link between these cytokines and the immune response.
- Activated EOS in the bone marrow can attack leukemic cells through mechanisms like adhesion and phagocytosis, and they express cytokines IL-12 and IL-17, indicating their potential role in combating leukemia.
[Pathological effects and clinical significance of eosinophils in autoimmune-related hematocytopenia].
Zhonghua Yi Xue Za Zhi
February 2013
Objective: To observe the pathological effects induced by eosinophils (EOS) in the process of cellular damage in immune-related hematocytopenia (IRH) and elucidate the immunologic mechanism and clinical significance of EOS.
Methods: Enzyme-linked immunosorbent assay (ELISA) was performed to determine the serum concentrations of interleukins (IL)-2, IL-4, IL-5, IL-6, IL-12 and IL-17 in 117 IRH patients from February 2008 to February 2012 in our hospital. Their quantity, activity, peroxidase (POX) and HLA-DR expression of EOS were observed and analyzed.
Objective: To observe the telomerase activity (TA) in bone marrow mononuclear cell (BMMNC) at pre-therapy and post-therapy in patients with immune related hematocytopenia and immune related pancytopenia (IRH/IRP) so as to explore the relationship between TA and disease process.
Methods: TA in BMMNC of 108 IRH/IRP patients was detected with telomeric repeat amplification protocol (TRAP)-PCR-ELISA. The expressions of anti-human immunoglobulin IgG, FcγII receptor (FcγIIR), mannose receptor (MR) and interleukin 17A (IL-17A) located on membrane surface were observed by immunofluorescence (IF).