Ultrasound assisted enzymatic synthesis of OPO structured lipids bycovalent organic framework immobilizedlipase.

In this study, the covalent organic framework immobilized Rhizomucor miehie lipase COF@RML as a novel biocatalyst was applied in the enzymatic synthesis of OPO structured lipids (1, 3-dioleoyl-2-palmitoylglycerol). The impact of reaction medium, substrate molar ratio, enzyme addition amount, reaction time and temperature on the enzymatic synthesis of OPO structured lipids were studied. Furthermore, the effects of ultrasonic power and ultrasonic time on the synthesis of OPO structural lipids were studied.

Encapsulation of Immobilized β-Glucosidase with Calcium Metal-Organic Frameworks for Enhanced Stability in Hydrolysis of Cellobiose.

β-Glucosidase (β-G) holds promising applications in various fields, such as biomass energy, food, pharmaceuticals, and environmental protection, yet its industrial application is still limited by issues of stability and recycling. Herein, we first immobilized β-G onto the surface of magnetic chitosan nanoparticles (MCS/β-G) through adsorption methods. Subsequently, utilizing the metal-organic framework (MOF), CaBDC, which possesses good stability under acidic conditions, we encapsulated MCS/β-G.

Tailoring the interfacial microenvironment of magnetic metal-organic frameworks using amino-acid-based ionic liquids for lipase immobilization.

Modifying the carrier interface is a promising method to improve the microenvironment of immobilized enzymes and enhance their activity and stability. In this work, using proline as amino acid, magnetic metal-organic frameworks (MOFs) were modified with an amino-acid-based ionic liquid (AAIL) with two hydroxyl groups followed by adsorption of porcine pancreatic lipase (PPL). The activity recovery of the prepared immobilized lipase (MMOF-AAIL/PPL) was up to 162 % higher than that of MMOF-PPL (70.

Lipase in-situ immobilized in covalent organic framework: Enzymatic properties and application in the preparation of 1, 3-dioleoyl-2-palmitoylglycerol.

In this study, the critical importance of designing an appropriate immobilized carrier and method for free lipase to ensure exceptional biological catalytic activity and stability was emphasized. Covalent organic frameworks (COF-1) were synthesized as a novel porous carrier with an azine structure (-CN-NC-) through the condensation of hydrazine hydrate and benzene-1,3,5-tricarbaldehyde at room temperature. Simultaneously, Rhizomucor miehei lipase (RML) was immobilized within the COF-1 carrier using an in-situ aqueous phase method.

Calcium-based MOFs as scaffolds for shielding immobilized lipase and enhancing its stability.

The enzyme immobilization technology has become a key tool in the field of enzyme applications; however, improving the activity recovery and stability of the immobilized enzymes is still challenging. Herein, we employed a magnetic carboxymethyl cellulose (MCMC) nanocomposite modified with ionic liquids (ILs) for covalent immobilization of lipase, and used Ca-based metal-organic frameworks (MOFs) as the support skeleton and protective layer for immobilized enzymes. The ILs contained long side chains (eight CH units), which not only enhanced the hydrophobicity of the carrier and its hydrophobic interaction with the enzymes, but also provided a certain buffering effect when the enzyme molecules were subjected to compression.

Covalent immobilization of lipase on an ionic liquid-functionalized magnetic Cu-based metal-organic framework with boosted catalytic performance in flavor ester synthesis.

Enzymatic esterification plays an important role in the fields of chemistry and biotechnology. In this study, lipase was immobilized on an ionic liquid (IL)-modified magnetic metal-organic framework (MOF) and used to synthesize isoamyl acetate. The immobilized lipase (PPL-ILs/FeO@MOF) showed 2.

Surface Modification of Magnetic ZIF-90 Nanoparticles Improves the Microenvironment of Immobilized Lipase and Its Application in Esterification.

Interactions of enzymes with supports significantly affect the activity and stability of immobilized enzymes. Herein, amino-functionalized ionic liquid (IL)-grafted magnetic zeolitic imidazolate framework-90 (MZIF-90) was prepared and used to immobilize porcine pancreatic lipase (PPL). The nanocomposites were fully characterized; meanwhile, the interactions between ILs and ZIF-90 were calculated based on density functional theory.

Preparation and Characterization of Magnetic Metal-Organic Frameworks Functionalized by Ionic Liquid as Supports for Immobilization of Pancreatic Lipase.

Enzymes are difficult to recycle, which limits their large-scale industrial applications. In this work, an ionic liquid-modified magnetic metal-organic framework composite, IL-FeO@UiO-66-NH, was prepared and used as a support for enzyme immobilization. The properties of the support were characterized with X-ray powder diffraction (XRD), Fourier-transform infrared (FTIR) spectra, transmission electron microscopy (TEM), scanning electronic microscopy (SEM), and so on.

Correction: Naphthalimide-containing coordination polymer with mechanoresponsive luminescence and excellent metal ion sensing properties.

Correction for 'Naphthalimide-containing coordination polymer with mechanoresponsive luminescence and excellent metal ion sensing properties' by Jian-Jun Liu , , 2020, , 3174-3180, DOI: 10.1039/C9DT04928B.

Enhancing bio-catalytic performance of lipase immobilized on ionic liquids modified magnetic polydopamine.

In this study, imidazolium-based ionic liquid with [tfN] as the anion was successfully grafted to magnetic polydopamine nanoparticles (MPDA). The prepared materials were well characterized and used as supports for lipase immobilization. The immobilized lipase (PPL-ILs-MPDA) exhibited excellent activity and stability.

Co-immobilization of laccase and ABTS onto amino-functionalized ionic liquid-modified magnetic chitosan nanoparticles for pollutants removal.

This work aims to achieve the co-immobilization of laccase and 2,2-binamine-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) to improve removal capability of the biocatalyst for pollutants while avoiding potential pollution caused by ABTS. The laccase was immobilized on magnetic chitosan nanoparticles modified with amino-functionalized ionic liquid containing ABTS (MACS-NIL) based on Cu ion chelation (MACS-NIL-Cu-lac). The carrier was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, x-ray diffraction and etc.

Naphthalimide-containing coordination polymer with mechanoresponsive luminescence and excellent metal ion sensing properties.

Mechanoresponsive luminescent materials coupled with other functionalities are of particular interest due to their multiple external stimuli responsive properties. In this paper, a new sensitive mechanoresponsive luminescent coordination polymer, [Cd(INI)(DMF)2·DMF] (1) (H2INI = N-(5-isophthalic acid)-1,8-naphthalimide), has been successfully designed and synthesized. Complex 1 exhibits interesting mechanoresponsive and grinding-enhanced luminescence properties, and its luminescence colour changed from weak blue-green to bright blue upon grinding owing to the external pressure-induced destruction of ππ stacked arrangements in local defective areas.

Ionic liquids-modified cellulose coated magnetic nanoparticles for enzyme immobilization: Improvement of catalytic performance.

In this work, ionic liquids-modified magnetic carboxymethyl cellulose nanoparticles (IL-MCMC) were prepared and used as supports for enzyme immobilization. The specific activity of immobilized lipase PPL-IL-MCMC was 1.43 and 2.

Design of GO-Ag-functionalized FeO@CS composite for magnetic adsorption of rhodamine B.

In this study, a novel magnetic composite (FeO@CS/GO/Ag) modified with chitosan (CS), graphene oxide (GO) and Ag nanoparticles (Ag NPs) was successfully prepared as an efficient adsorbent for detection of rhodamine B (RB) combined with a fluorescence technique. The properties of the magnetic composite were confirmed by field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, and vibrating sample magnetometry. The components of FeO@CS/GO/Ag endowed it with excellent extraction performance and convenient operation.

Enhanced catalytic performance of lipase covalently bonded on ionic liquids modified magnetic alginate composites.

We prepared ionic liquids (ILs) modified magnetic alginate nanoparticles and used these as supports for lipase immobilization. The novel supports were characterized using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (HNMR), vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and water contact angle (WCA) measurements. The immobilized lipase (PPL-IL-MSA) exhibited high activity, 2.

Synthesis of functional ionic liquid modified magnetic chitosan nanoparticles for porcine pancreatic lipase immobilization.

We developed magnetic chitosan nanoparticles (CS‑FeO) with mean diameter of 15-20 nm. Subsequently, these inorganic-organic composite nanoparticles were modified using an imidazole-based functional ionic liquid (IL). The prepared support (IL‑CS‑FeO), which was used to immobilize porcine pancreatic lipase (PPL), was characterized using Fourier transform infrared (FTIR) spectroscopy, vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and X-ray diffraction (XRD).

Enhancement of catalytic performance of porcine pancreatic lipase immobilized on functional ionic liquid modified FeO-Chitosan nanocomposites.

Magnetic chitosan nanocomposites were designed and fabricated by combining the magnetic nanoparticles FeO and chitosan covalently modified by imidazole-based ionic liquids with various functional groups, to be utilized as a support matrix for immobilization of porcine pancreatic lipase (PPL). Ionic liquids modified chitosan was characterized with nuclear magnetic resonance (NMR) and the nanocomposites were characterized with Fourier transform infrared spectroscopy (FTIR), vibrating-sample magnetometer (VSM), thermogravimetry analysis (TGA), Transmission electron microscope (TEM) and Scanning electron microscopy (SEM). The enzymatic properties of PPL were significantly improved by immobilization onto all thus prepared nanocomposites, and among the supports, modified with ionic liquids bearing hydroxyl group exhibited relatively enhanced performance.

Covalent immobilization of lipase onto chitosan-mesoporous silica hybrid nanomaterials by carboxyl functionalized ionic liquids as the coupling agent.

Chitosan-mesoporous silica SBA-15 hybrid nanomaterials (CTS-SBA-15) were synthesized by means of carboxyl functionalized ionic liquids as the coupling agent. The as-prepared CTS-SBA-15 support was characterized by TEM, FTIR, TG and nitrogen adsorption-desorption techniques. Porcine pancreas lipase (PPL) was then bound to the hybrid nanomaterials by using the cross-linking reagent glutaraldehyde (GA).

Fabrication of chitosan-mesoporous silica SBA-15 nanocomposites via functional ionic liquid as the bridging agent for PPL immobilization.

A nanocomposite (SBA-CIL-CS) which was combined mesoporous silica SBA-15 material with chitosan via ionic liquid as the bridging agent was successfully fabricated. The morphology and structure of the nanocomposite were characterized in detail with transmission electron microscope, fourier transform infrared spectroscopy, thermogravimetric analysis and nitrogen adsorption-desorption techniques. SBA-CIL-CS was investigated as an efficient support for immobilization of porcine pancreas lipase (PPL) and possessed high immobilization efficiency.