Publications by authors named "Hongbo Gao"

Dynamic DNA methylation represses transposable elements (TEs) and regulates gene activity, playing a pivotal role in plant development. Although substantial progress has been made in understanding DNA methylation reprogramming during germline development in Arabidopsis thaliana, whether similar mechanisms exist in other dicot plants remains unclear. Here, we analyzed DNA methylation levels in meiocytes, microspores, and pollens of Brassica Rapa using whole-genome bisulfite sequencing (WGBS).

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Kawasaki disease (KD) is the common acute, self-limiting vasculitis in children, often affecting coronary arteries, which may lead to coronary artery dilation, stenosis, or in severe cases, myocardial infarction. This study aimed to identify new approaches for reducing or preventing coronary artery lesions (CAL) in KD patients by analyzing specific serological markers across various paediatric groups. Clinical data were collected from 100 children diagnosed with Kawasaki disease (KD) admitted at First Affiliated Hospital of Hebei North University between May 2023 and June 2024.

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Ultrasound and X-ray are considered potential options in cancer therapy due to their superior tissue penetration capabilities. Developing effective radiosensitizers is crucial for advancing cancer treatment as they enhance the effectiveness of radiation and sonodynamic therapies while minimizing their side effects. This study aimed to design a degradable bismuth-based heterojunction BiF-BiO:S-PEG (BFOSP) radiosensitizer using anion-exchange-regulated charge separation engineering.

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Immune checkpoint blockade (ICB) has achieved encouraging outcome in various malignant tumors. However, the low immunogenicity and insufficient infiltration of T cells within tumors severely limit the curative effects. Herein, we reported synthesis and experimental evaluation of HO-responsive MnTe nanosheets (NSs) for improving anti-tumor immune responses.

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Two isostructural thiacalix[4]arene-capped Ln aggregates with a "hand in hand" structure, denoted as {Ln(μ-OH)Cl(TC4A)(BCT)(DMF)(CHOH)(HO)}·CHOH·DMF (Ln = Tb (1), Eu (2); HTC4A = -butylthiacalix[4]arene; HBCT = 3,5-bis(4'-carboxy-phenyl)-1,2,4-triazole; DMF = ,'-dimethylformamide), were constructed from two sandwich-like Ln-(TC4A) entities bridged two BCT linkers. These aggregates present a layer-like structure on the plane, with poly-nuclear secondary building units (PSBUs) staggered and assembled in three-dimensional space. 1 exhibits green photoluminescence under 379 nm excitation, with an average decay time of approximately 1.

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Gleditsia sinensis Lam. (G. sinensis) as an important species within the Leguminosae family, has been utilized in Chinese medicine for centuries, and its thorns serve as a chief medicinal ingredient.

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Background: This study aimed to develop and validate a multiregional radiomic-based composite model to predict symptomatic radiation pneumonitis (SRP) in non-small cell lung cancer (NSCLC) patients treated with stereotactic body radiation therapy (SBRT).

Materials And Methods: 189 patients from two institutions were allocated into training, internal validation and external testing cohorts. The associations between the SRP and clinic-dosimetric factors were analyzed using univariate and multivariate regression.

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Soil borne diseases are one of the most serious diseases which often results the decline of vegetables quality and loss of production. Moreover, it is difficult for plants to exhibit disease symptoms in the early stages attributing to strong concealment of soil borne pathogens. Therefore, early detection of pathogens and their physiological races plays an important role in reducing the harm of pathogens associated with diseases of vegetable crops.

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Objective: The aim of this study was to investigate the relationship between tumor microenvironment markers (myeloid-derived suppressor cells [MDSCs], regulatory T cells [Tregs], programmed cell death 1 [PD-1], and programmed death ligand 1 [PD-L1]) and chemotherapy efficacy and prognosis in advanced gastric cancer, identifying potential monitoring indicators.

Methods: Advanced gastric cancer patients' MDSC and Treg expression was measured by flow cytometry pre- and postchemotherapy; PD-1 and PD-L1 expression in cancer tissues was assessed by immunohistochemistry. Correlations with chemotherapy outcomes and prognosis were analyzed.

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Article Synopsis
  • - This study focuses on how takeover time (TOT) influences decision-making in Level-3 autonomous vehicles (L3-AVs), highlighting gaps in current research regarding TOT's impact on safety and trajectory planning.
  • - The researchers developed a new model called the exponential smoothing transformers (ETSformer) for predicting TOT, which was shown to accurately capture over 83% of TOT characteristics and improve decision-making by reducing errors.
  • - Findings indicate that understanding TOT is crucial for enhancing the safety and stability of L3-AVs, providing insights that could lead to better decision-making strategies in various driving conditions.
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  • Soils with high salt content pose a significant challenge to plant growth, leading to a focus on improving salt tolerance in crops.
  • Exogenous substances like plant hormones can help plants manage salt stress by regulating ion transport and reducing harmful reactive oxygen species (ROS).
  • This review discusses how these substances work and suggests future research directions to better understand and enhance salt tolerance in plants.
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  • The study focuses on predicting the risk of hepatocellular carcinoma (HCC) in patients with chronic hepatitis B receiving nucleos(t)ide analogues (NAs), emphasizing the importance of serum hepatitis B virus (HBV) RNA as a novel biomarker.
  • Researchers analyzed data from 1,374 NA-treated patients, tracking HBV RNA levels over three years and using a Cox proportional-hazard model to assess the link between HBV RNA declines and HCC risk, finding significant associations.
  • The findings suggest that patients with lower declines in HBV RNA at one and two years have a higher risk of developing HCC, and incorporating these declines into existing risk prediction models can improve their accuracy and help guide patient monitoring strategies
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Hybrid breeding between herbaceous peonies (the maternal parent) and tree peonies (the paternal parent) results in Paeonia Itoh hybrids (Itoh peonies), a triploid species that combines advantageous traits from both parental species, thus offering great economic value. However, the exact genetic contribution of the two parents is unclear. In this study, we introduce a straightforward approach utilizing heterozygous single-nucleotide polymorphisms (SNPs) and Sanger sequencing of targeted gene fragments to trace the original bases back to their parents in Itoh peonies.

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Background: Bivalirudin, a direct thrombin inhibitor, is used in anticoagulation therapies as a substitute for heparin, especially during cardiovascular procedures such as percutaneous coronary intervention.

Aim: To explore the effect of bivalirudin on myocardial microcirculation following an intervention and its influence on adverse cardiac events in elderly patients with acute coronary syndrome (ACS).

Methods: In total, 165 patients diagnosed with acute myocardial at our hospital between June 2020 and June 2022 were enrolled in this study.

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Article Synopsis
  • Heat shock protein 20 (Hsp20) is essential for lettuce's response to drought stress, but its gene family is not well understood, prompting a study that identified 36 members of this family.
  • Analysis revealed that most LsHsp20 proteins are located in the cytoplasm and nucleus, and a variety of structural traits were characterized, including conserved domains and gene structures showing intron variations.
  • Expression studies demonstrated significant upregulation of certain genes under drought conditions, indicating their potential regulatory roles in adaptation and stress response, laying groundwork for further research on LsHsp20's biological functions.
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N-methyladenosine (mA) RNA modification is the most prevalent messenger RNA (mRNA) modification in eukaryotes and plays critical roles in the regulation of gene expression. mA is a reversible RNA modification that is deposited by methyltransferases (writers) and removed by demethylases (erasers). The function of mA erasers in plants is highly diversified and their roles in cereal crops, especially in reproductive development essential for crop yield, are largely unknown.

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Filamentous temperature-sensitive Z (FtsZ) is a tubulin-like GTPase that is highly conserved in bacteria and plants. It polymerizes into a ring at the division site of bacteria and chloroplasts and serves as the scaffold protein of the division complex. While a single FtsZ is present in bacteria and cyanobacteria, there are two subfamilies, FtsZ1 and FtsZ2 in the green lineage, and FtsZA and FtsZB in red algae.

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Drought stress is one of the most critical threats to crop productivity and global food security. This review addresses the multiple effects of drought on the process of photosynthesis in major food crops. Affecting both light-dependent and light-independent reactions, drought leads to severe damage to photosystems and blocks the electron transport chain.

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Article Synopsis
  • The study focuses on creating a prognostic model to predict HBsAg loss in patients with chronic hepatitis B (CHB) undergoing antiviral treatment, which currently rarely occurs with existing therapies.
  • Data from 6,792 patients treated with nucleos(t)ide analogues were analyzed, leading to the development of the GOLDEN model that integrates longitudinal HBsAg measurements.
  • The GOLDEN model demonstrated high accuracy in predicting HBsAg clearance, effectively identifying patients likely to achieve this outcome, which could improve patient stratification in future treatments.
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Immunofluorescence staining with frozen sections of plant tissues and a nest tube is convenient and effective, and broadens the applicability of immunofluorescence staining. Immunofluorescence staining is an indispensable and extensively employed technique for determining the subcellular localization of chloroplast division proteins. At present, it is difficult to effectively observe the localization of target proteins in leaves that are hard, or very thin, or have epidermal hair or glands with the current immunofluorescence staining methods.

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Article Synopsis
  • Various reporter genes, like the RUBY reporter gene, help researchers study how genes are expressed and regulated, but there was a gap in accurately measuring RUBY expression levels.
  • This study presents a new method for quantifying betalain produced in tobacco leaves, enabling precise measurement and analysis of gene expression, specifically focusing on the regulation of the ARC5 gene and the effects of light on other genes.
  • The introduced method is cost-effective and user-friendly, making it easier to explore the mechanisms that control gene expression.
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Membrane transporters are proteins that mediate the entry and exit of substances through the plasma membrane and organellar membranes and are capable of recognizing and binding to specific substances, thereby facilitating substance transport. Membrane transporters are divided into different types, e.g.

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