Molecular Characteristics and Role of Buffalo in Triglyceride and Cholesterol Biosynthesis in Mammary Epithelial Cells.

Sterol regulatory element-binding transcription factor 2 (SREBF2) is a key transcription factor involved in regulating cholesterol homeostasis. However, its role in buffalo mammary gland lipid metabolism remains unclear. : To address this, we isolated and characterized the gene from buffalo mammary glands and performed an in-depth analysis of its molecular characteristics, tissue-specific expression, and functional roles in buffalo mammary epithelial cells (BuMECs).

Leveraging the gut microbiome to understand the risk factor of cognitive impairment in patients with liver cirrhosis.

Objectives: The role of the gut-liver axis in liver cirrhosis is becoming increasingly recognized. We investigated the fecal microbiome in patients with liver cirrhosis and its potential function as a predictive biomarker of hepatic encephalopathy.

Methods: Patients were divided into either a high plasma ammonia (HPA) group or a low plasma ammonia (LPA) group according to the upper limit of normal of plasma ammonia concentration.

Effects of Ganoderma lucidum fermentation on the structure of Tartary buckwheat polysaccharide and its impact on gut microbiota composition.

Fermentation modifies plant polysaccharides and enhances their bioactivity. In this study, polysaccharides were extracted from Ganoderma lucidum-fermented (FBP) and non-fermented (NBP) Tartary buckwheat. The structure of the polysaccharides and their effects on gut microbial composition were explored.

Molecular turn in pathogenesis: implications of the frameshift for bacterial survival in human macrophage.

, the etiological agent of the devastating plague, has caused three pandemics in human history. While known for its fatality, it has long been intriguing that biovar microtus strains are highly attenuated to humans. The survival and replication within macrophages are critical in the early stages of the lifestyle within warm-blooded hosts.

The therapeutic effect of leucogen in treating alcoholic liver cirrhosis with thrombocytopenia or leukopenia.

Background: Thrombocytopenia and leukopenia are common complications of alcoholic liver cirrhosis (ALC) and are associated with an increased risk of bleeding, infection and mortality.

Objectives: Evaluate the effectiveness and safety of leucogen, a cysteine derivative that increases platelet (PLT) and white blood cell (WBC) counts in ALC patients.

Design: Retrospective.

Distinct cellular mechanisms underlie chemotherapies and PD-L1 blockade combinations in triple-negative breast cancer.

Combining immune checkpoint blockade (ICB) with chemotherapy shows promise for treating triple-negative breast cancer (TNBC), though the mechanisms remain incompletely understood. Here, we integrate published and new single-cell RNA sequencing (scRNA-seq) data to investigate the tumor immune microenvironment (TIME) in TNBC patients treated with paclitaxel (PTX), nab-paclitaxel (Nab-PTX), and their combinations with the anti-PD-L1 antibody atezolizumab (ATZ). Compared to ATZ plus PTX, ATZ plus Nab-PTX rewires TCF7 stem-like effector memory CD8 T cells (Tsem) and CD4 T follicular helper (Tfh) cells.

A phytoplasma effector suppresses insect melanization immune response to promote pathogen persistent transmission.

Insect melanization triggered by the conversion of prophenoloxidase to active phenoloxidase via serine proteases (SPs) is an important immediate immune response. However, how phytoplasmas evade this immune response to promote their propagation in insect vectors remains unknown. Here, we demonstrate that infection of leafhopper vectors with rice orange leaf phytoplasma (ROLP) activates the mild melanization response in hemolymph.

Clinical and ultrasound features of fibrous pseudotumor of tunica vaginalis of the testis: eight cases and literature review.

Objective: To investigate the clinical and ultrasound features of fibrous pseudotumor of tunica vaginalis of the testis.

Methods: The clinical and ultrasound features of fibrous pseudotumor of the tunica vaginalis diagnosed by pathology in West China Hospital of Sichuan University from 2006 to 2023 were retrospectively analyzed.

Results: The study included 8 patients diagnosed with fibrous pseudotumor of the tunica vaginalis.

Rapid detection of by recombinase-aided amplification combined with the CRISPR/Cas12a system.

() is one of the primary agents involved in porcine respiratory disease complex, and circulates in the swine industry worldwide. The prevention and control of is complicated. Thus, a recombinase-aided amplification (RAA) assay coupled with the clustered regularly-interspaced short palindromic repeats (CRISPR)/Cas12a system was established for the detection of .

Covert Perioperative Strokes in Older Patients Having Noncardiac Surgery (PRECISION): A Prospective Cohort Analysis.

Background: Perioperative strokes may promote postoperative neurocognitive dysfunction. This study thus evaluated the incidence of postoperative strokes and the association between strokes and postoperative neurocognitive outcomes in older patients recovering from noncardiac surgery.

Methods: The Postoperative Covert Stroke and Cognitive Dysfunction among Elderly Patients Undergoing Noncardiac Surgery study (PRECISION) was a two-center prospective cohort study evaluating patients aged 60 yr or older who had elective, noncardiac inpatient surgery at two Chinese academic centers.

Hydrogel-based nonwoven with persistent porosity for whole-stage hypertonic wound healing by regulating of water vaporization enthalpy.

Moisture induced by wound exudate is crucial throughout the wound repair process. The dressing directly affects the absorption, permeation, and evaporation of the wound exudate. However, most dressings in clinical often result in excessive dryness or moisture of wound due to their monotonous structure and function, leading to ineffective thermodynamic control of evaporation enthalpy.

Evaluation study of congelex laxative granules based on HPLC fingerprint, multi-component content determination, and chemometrics.

Congelex Laxative Granules is an in-house preparation of Hebei Provincial Hospital of Traditional Chinese Medicine. This study aims to establish the HPLC fingerprint of Congelex Laxative Granules and evaluate its quality using chemometric methods. The Agilent Eclipse Plus C18 column and a methanol-water gradient elution system were employed, with detection at 224 nm.

Functional crosstalk of sucrose and G protein signaling in maize thermotolerance by modulating osmoregulation system.

Sucrose (SUC) is a signaling molecule with multiple physiological functions. G protein is a kind of receptor that converts extracellular first messenger into intracellular second messenger. However, it is little known that SUC interplays with G protein signaling in maize thermotolerance.

MR perfusion characteristics of pseudoprogression in brain tumors treated with immunotherapy - a comparative study with chemo-radiation induced pseudoprogression and radiation necrosis.

Purpose: Pseudoprogression is an atypical imaging pattern of response to immunotherapy in patients with brain tumors. MR perfusion studies in this field are limited. The purpose of our study is to compare the perfusion features between pseudoprogression lesions in malignant gliomas and brain metastases treated with immunotherapy (iPsP) and the pseudoprogression after chemo-radiation therapy and radiation necrosis after radiation treatment (ChR-PsP & RN).

A comprehensive neuroimaging review of the primary and metastatic brain tumors treated with immunotherapy: current status, and the application of advanced imaging approaches and artificial intelligence.

Cancer immunotherapy has emerged as a novel clinical therapeutic option for a variety of solid tumors over the past decades. The application of immunotherapy in primary and metastatic brain tumors continues to grow despite limitations due to the physiological characteristics of the immune system within the central nervous system (CNS) and distinct pathological barriers of malignant brain tumors. The post-immunotherapy treatment imaging is more complex.

Development and application of a quadruplex real-time fluorescence quantitative PCR assay for four porcine digestive pathogens.

Introduction: , , , and are the primary pathogens responsible for gastrointestinal diseases in pigs, posing a significant threat to the health and productivity of pig production systems. Pathogen detection is a crucial tool for monitoring and managing these infections.

Methods: We designed primers and probes targeting the gene of , the 23S gene of , the gene of , and the gene of .

Development and application of a quadruplex TaqMan fluorescence quantitative PCR typing method for generalis, type 2, type 7 and type 9.

Introduction: (SS) is one of the most important pathogens causing major economic losses in the global pig farming industry and is a serious threat to public health safety. It has multiple serotypes, with poor cross-protection between serotypes, and effective typing methods are lacking.

Methods: In this study, a quadruplex TaqMan fluorescence quantitative PCR assay that can differentiate between types 2, 7 and 9 was developed using the gene, a generic gene for , and , and , genes encoding podocarp-associated genes for types 2, 7 and 9, respectively, as targets.

Competitive adsorption of polycyclic aromatic hydrocarbons on phosphorus tailing-modified sludge biochar provides mechanistic insights.

Biochar has been widely used to solve the wastewater pollution of polycyclic aromatic hydrocarbons (PAHs). However, the competition of PAHs with different benzene ring numbers (e.g.

Development of a Triplex qPCR Assay Based on the TaqMan Probe for the Detection of , Serotype 2 and .

Porcine respiratory disease is a significant economic problem for the global swine industry. (), (), and () are three important pathogenic bacteria of the swine respiratory tract. Notably, the three pathogens not only frequently manifest as mixed infections, but their striking clinical similarities also present difficulties for pig populations in terms of disease prevention and treatment.

TMSB4Y restrains sphingomyelin synthesis via de novo purine synthesis to exert a tumor suppressor function in male esophageal squamous cell carcinoma.

Y chromosome genes play a vital role in sex difference of cancer. The dysregulation and functional implications of Y chromosome genes in esophageal squamous cell carcinoma (ESCC) remains elusive. Here, we analyze the Y chromosome gene signature and identify TMSB4Y as an emerging prognostic predictor in male ESCC.