Enhanced Discovery of Alternative Proteins (AltProts) in Mouse Cardiac Development Using Data-Independent Acquisition (DIA) Proteomics.

Alternative proteins (AltProts) are a class of proteins encoded by DNA sequences previously classified as noncoding. Despite their historically being overlooked, recent studies have highlighted their widespread presence and distinctive biological roles. So far, direct detection of AltProt has been relying on data-dependent acquisition (DDA) mass spectrometry (MS).

Development and Applications in Intelligent Sports of Hydrogel-Based Triboelectric Nanogenerators.

As an emerging self-powered technology, triboelectric nanogenerators have the characteristics of a simple structure, high conversion efficiency, diverse material selection, and stable output. Hydrogels have the advantages of flexibility, extensibility, and shape adaptability, which means that hydrogel-based triboelectric nanogenerators (H-TENGs) have high flexibility, self-healing abilities, conductivity, and fatigue resistance. They can still operate normally in scenarios involving bending, pressing, stretching, and folding.

Insect-inspired passive wing collision recovery in flapping wing microrobots.

Flying insects have developed two distinct adaptive strategies to minimize wing damage during collisions. One strategy includes an elastic joint at the leading edge, which is evident in wasps and beetles, while another strategy features an adaptive and deformable leading edge, as seen in bumblebees and honeybees. Inspired by the latter, a novel approach has been developed for improving collision recovery in micro aerial vehicles (MAVs) by mimicking the principle of stiffness anisotropy present in the leading edges of these insects.

Fluoride-Induced Autophagy and Apoptosis in the Mouse Ovary: Genomic Insights into IL-17 Signaling and Gut Microbiota Dysbiosis.

Chronic fluoride (F) exposure is linked to gonadotoxicity in females, yet the underlying molecular mechanisms remain unclear. This study investigated fluoride-induced reprotoxicity using advanced genomic profiling. RNA-seq analysis identified significant activation of autophagy, apoptosis, and IL-17 signaling pathways in fluoride-exposed female mice.

Effect of (-)-epigallocatechin gallate palmitate complexation under mild temperature on the structure and nutritional functions of porous rice starch.

The correlation among the hierarchical structure, physicochemical properties, and nutritional functions of porous rice starch after absorbing and complexing with (-)-epigallocatechin gallate palmitate (P-EGCG) under mild temperatures at different reaction times were investigated. The P-EGCG loading rate (19.6 %-28.

Intraspecific Chloroplast Genome Genetic Polymorphism of (Xi Junecry) and Its Revelation of a Single Origin in Phylogeny.

: Xi Junecry (), a perennial herb of the Araceae family, is indigenous to Xinxian County, Henan Province, China, and is regarded as a premium variety among similar medicinal materials. However, the lack of comprehensive genetic information on Xi Junecry germplasm resources has constrained the cultivation and identification of high-quality varieties. : In this study, six chloroplast genomes of Xi Junecry were assembled and annotated using high-throughput sequencing.

Antibacterial mechanism of SHY96 cell-free supernatant against revealed by metabolomics and potential application on chicken breast meat preservation.

The cell-free supernatant of (LCFS) is considered a potential natural antimicrobial agent due to its outstanding antimicrobial activity. This study demonstrated that the cell-free supernatant of SHY96 (LCFS96) effectively inhibits the growth and biofilm formation of CMCC(B)54002 (_02) by reducing cell metabolic activity and damaging cell structure. Metabolomic analysis revealed that LCFS96 significantly altered 450 intracellular metabolites, affecting key metabolic pathways including linoleic acid metabolism, pyrimidine metabolism, purine metabolism, pantothenic acid and CoA biosynthesis, and the TCA cycle.

Risk factors for noncontiguous spinal fractures among elderly patients presenting with traumatic spinal fractures: a retrospective case‒control study.

Objective: Risk factors for noncontiguous spinal fractures (NSFs), which are important for early, timely diagnoses and treatments of elderly individuals with traumatic spinal fractures, have not been discussed in depth. Thus, this study aimed to investigate the risk factors for noncontiguous spinal fractures (NSFs).

Methods: The records of 1,415 elderly patients (aged 60 years and over) who presented with TSFs between 2013 and 2019 were retrospectively reviewed.

Measurement of optical density of microbes by multi-light path transmission method.

Optical density (OD) is an important indicator of microbial density, and a commonly used variable in growth curves to express the growth of microbial culture. However, OD values show a linear relationship with bacterial concentration only at low concentrations. When the cell density is high, the relationship loses linearity, and serial dilution is needed to obtain readings of better accuracy.

Single-cell RNA sequencing identifies the expression of hemoglobin in chondrocyte cell subpopulations in osteoarthritis.

In recent years, chondrocytes have been found to contain hemoglobin, which might be an alternative strategy for adapting to the hypoxic environment, while the potential mechanisms of that is still unclear. Here, we report the expression characteristics and potential associated pathways of hemoglobin in chondrocytes using single-cell RNA sequencing (scRNA-seq). We downloaded data of normal people and patients with osteoarthritis (OA) from the Gene Expression Omnibus (GEO) database and cells are unbiased clustered based on gene expression pattern.

Chronic myelogenous leukemia coexpressing V-e16a2, V-e13a2, e13a2, and e14a2 fusion transcripts: a case report and review of the literature.

The coexistence of three or more transcripts in one patient with chronic myeloid leukemia (CML) is rarely reported. Thus, the disease progression and drug response are still unknown. This case report aimed to explore the drug response of CML with variant transcripts and to enrich the clinical treatment of rare types of CML.

Disrupted methionine cycle triggers muscle atrophy in cancer cachexia through epigenetic regulation of REDD1.

The essential amino acid methionine plays a pivotal role in one-carbon metabolism, facilitating the production of S-adenosylmethionine (SAM), a critical supplier for DNA methylation and thereby a modulator of gene expression. Here, we report that the methionine cycle is disrupted in skeletal muscle during cancer cachexia, leading to endoplasmic reticulum stress and DNA hypomethylation-induced expression of the DNA damage inducible transcript 4 (Ddit4) gene, encoding the regulated in development and DNA damage response 1 (REDD1) protein. Targeting DNA methylation by depletion or pharmacological inhibition of DNA methyltransferase 3A (DNMT3A) exacerbates cachexia, while restoring DNMT3A expression or REDD1 knockout alleviates cancer cachexia-induced skeletal muscle atrophy in mice.

Inhibiting FGFR by toadflax reverses erlotinib resistance in nonsmall cell lung cancer.

This study aims to demonstrate the effect of toadflax (bufalin) on erlotinib resistance in nonsmall cell lung cancer (NSCLC) by inhibiting the fibroblast growth factor receptor (FGFR). The microfluidic mobility transferase and caliper mobility-shift assays were employed to detect the FGFR inhibition by bufalin and the binding reversibility. Further, the inhibitory effects of bufalin were determined in HCC827 and HCC827/ER cells in vitro, investigating relative FGFR overexpression by quantitative reverse transcriptase-PCR (RT-qPCR) and FGFR downstream proteins, that is, FGFR substrate 2 (FRS2), extracellular signal-regulated kinase (ERK), and S6 by western blot analysis.

Custom-Made Ce-Mn Bimetallic Nanozyme for the Treatment of Intervertebral Disc Degeneration by Inhibiting Oxidative Stress and Modulating Macrophage M1/M2 Polarization.

Intervertebral disc degeneration (IDD)-induced lower back pain (LBP) brings heavy burden worldwide. In the degenerated intervertebral disc, there is an increase in the accumulation of reactive oxygen species (ROS) and the infiltration of M1 macrophages, which leads to abnormal local inflammatory microenvironment and exacerbates IDD. In this study, we developed a novel injectable polyethylene glycol (PEG)-capped cerium ion-manganese ion (Ce-Mn) bimetallic nanozyme (CeMn-PEG) with strong ROS scavenging and M2-type macrophage polarizing abilities to efficiently alleviate IDD.

Imbalance of muscles around the cervical spine in patients with degenerative cervical spondylotic kyphosis and myelopathy.

Background: To measure the muscle strength around the cervical spine; clarify the relationships among muscles, cervical sagittal alignment, and cervical spondylotic myelopathy(CSM); and understand the process underlying loss of cervical lordosis.

Methods: Sex, age, course of illness, and radiological data were obtained for patients with CSM and a control group of healthy individuals. C2-7 Cobb angles were measured in cervical radiographs, and the vertebral body areas(VBAs) and cross-sectional areas(CSAs) of the deep flexors, superficial flexors(SF), deep extensors, and superficial extensors(SE) were measured from the C3/4 to C6/7 intervertebral levels in T2-weighted axial magnetic resonance images.

The nuclear condensates of ESE3/EHF induce cellular senescence without the associated inflammatory secretory phenotype in pancreatic ductal adenocarcinoma.

Senescent cells are in a stable state of cell cycle arrest, leading to a natural barrier to tumorigenesis. Senescent cells secrete a pool of molecules, including cytokines, chemokines, proteases, and growth factors, termed the senescence-associated secretory phenotype (SASP), paradoxically contributing to pro-tumorigenic processes. However, the mechanism for regulating senescence and SASP in tumor cells remains unclear.

TRPC6 is a Biomarker for Prognosis and Immunotherapy of Stomach Adenocarcinoma Based on Bioinformatic Analysis and Experimental Validation.

Background: Transient receptor potential canonical 6 (TRPC6), a key member of the TRPC family, is involved in diverse physiological and pathological processes. Although previous studies have implicated TRPC6 in the progression of stomach adenocarcinoma (STAD), its precise functions and mechanisms remain unclear. Understanding TRPC6's role in STAD may provide insights into its prognostic and therapeutic potential.

Enhancing Methylene Blue Adsorption Performance of TiCT@Sodium Alginate Foam Through Pore Structure Regulation.

Pore structural regulation is expected to be a facile way to enhance the adsorption performance of MXene. In this work, spherical foam composites consisting of TiCT and sodium alginate (SA) were synthesized via a vacuum freeze-drying technique. By varying the solution volume of TiCT, four distinct TiCT@SA spherical foams with honeycomb-like and lamellar structures with a pore diameter in the range of 100-300 μm were fabricated.

Thermally tunable add-drop filter based on valley photonic crystals for optical communications.

Valley photonic crystals (VPCs) provide an intriguing approach to suppress backscattering losses and enable robust transport of light against sharp bends, which could be utilized to realize low-loss and small-footprint devices for on-chip optical communications. However, there are few studies on how to achieve power-efficient tunable devices based on VPCs, which are essential for implementing basic functions such as optical switching and routing. Here, we propose and experimentally demonstrate a thermally tunable add-drop filter (ADF) based on VPCs operating at telecommunication wavelengths.

Promoted growth with dynamic cellular stoichiometry driven by utilization of in-situ dissolved organic matter: Insights from bloom-forming dinoflagellate Prorocentrum donghaiense.

Mixotrophic dinoflagellates frequently cause harmful algal blooms (HABs) in eutrophic waters that contain diverse dissolved organic matter (DOM), especially intensive mariculture areas. Compared to the extensive investigation of phagotrophy and single organic molecule uptake by causative species, we have limited knowledge about the capability of mixotrophic dinoflagellates to utilize in-situ DOM in mariculture waters and its contribution to HABs. Here we use filtered in-situ mariculture water as the sole medium to examine the physiological response of Prorocentrum donghaiense to the natural mariculture DOM.

Liver cirrhosis complicated with pulmonary Nocardia infection: A case report and literature review.

Rationale: Nocardia is a conditionally pathogenic bacterium found in the natural environment and is an aerobic bacterium, which causes severe pneumonia relatively rarely. The diagnosis of primary Nocardia infection is always made late due to the nonspecific clinical presentation of patients with Nocardia infection and the time required for Nocardia culture. Due to its rarity and nonspecific clinical presentation, patient survival is often compromised due to misdiagnosis.

Asperosaponin VI inhibition of DNMT alleviates GPX4 suppression-mediated osteoblast ferroptosis and diabetic osteoporosis.

Introduction: Diabetic osteoporosis (DOP) is an insidious complication of diabetes with limited therapeutic options. DOP is pathologically associated with various types of regulated cell death, but the precise role of ferroptosis in the process remains poorly understood. Asperosaponin VI (AVI), known for its clinical efficacy in treating bone fractures and osteoporosis, may exert its osteoprotective effects through mechanisms involving ferroptosis, however this has not been established.

3D printing of stiff, tough, and ROS-scavenging nanocomposite hydrogel scaffold for in situ corneal repair.

Despite significant advancements in hydrogels in recent years, their application in corneal repair remains limited by several challenges, including unfitted curvatures, inferior mechanical properties, and insufficient reactive oxygen species (ROS)-scavenging activities. To address these issues, this study introduces a 3D-printed corneal scaffold with nanocomposite hydrogel consisting of gelatin methacrylate (GelMA), poly (ethylene glycol) diacrylate (PEGDA), Laponite, and dopamine. GelMA and PEGDA act as matrix materials with photo-crosslinking abilities.