Ultrasensitive Lateral Flow Immunoassay of Fluoroquinolone Antibiotic Gatifloxacin Using Au@Ag Nanoparticles as a Signal-Enhancing Label.

Gatifloxacin (GAT), an antibiotic belonging to the fluoroquinolone (FQ) class, is a toxicant that may contaminate food products. In this study, a method of ultrasensitive immunochromatographic detection of GAT was developed for the first time. An indirect format of the lateral flow immunoassay (LFIA) was performed.

A smartphone-based dual-modal lateral flow immunochromatographic assay for multiplex detection of illegal stimulant laxatives in slimming foods.

Stimulant laxatives (especially bisacodyl and sodium picosulfate) are frequently found to be adulterated into slimming foods, causing health-threatening effects to consumers. Sensitive, accurate, easy-to-operate and portable multiplex analytical techniques are still desired for the rapid screening of stimulant laxatives in slimming foods. In this work, a highly sensitive dual-modal colorimetric/photothermal lateral flow immunochromatographic assay (LFIA) was established based on facilely prepared concentrated gold nanoparticles (cAuNPs).

Identification and quantitation of multiplex camellia oil adulteration based on 11 characteristic lipids using UPLC-Q-Orbitrap-MS.

Currently, the identification and quantification of complex adulteration of high-value vegetable oils are still challenging. In this study, the extreme vertex design method was adopted to design representative multivariate adulterated camellia oil samples. Thereafter, 11 characteristic lipid species were identified by considering the statistically significant difference and categorical contribution.

Exploring the gelation potentials of chicken heart batter: From by-product to product.

Chicken heart is the by-product of great amount in poultry industry owing to concentrated slaughter development, however, whose potential in food application is unclear. The gelation of chicken heart has great difficulties due to the muscle structure and protein structure of the heart. Therefore, different levels of sodium alginate (SA) were added into chicken heart batter (CHB) to investigate the gelation strategies.

MXene Bimetallic Coating Synergistic Enhanced Colorimetric-Raman Dual Signal-Based Immunochromatographic Assay for Advancing Detection Performance.

Herein, a three-dimensional thin film-like multifunctional MXene bimetallic coating material (TiC@Au-Ag) with strong color intensity, high surface-enhanced Raman scattering (SERS) activity, and strong antibody affinity (1.00 × 10 M) was prepared. It was the first time that TiC@Au-Ag-based colorimetric-SERS dual-signal immunochromatographic assay (ICA) was developed for the detection of dexamethasone, achieving the limits of detection of 0.

A non-classical view of antibody properties: Allosteric effect between variable and constant regions.

Historically, antibodies have been divided into two functionally independent domains, the variable (V) region for antigen binding and the constant (C) region for mediating effector functions. However, this classical view of antibody function has been severely challenged by a large and growing number of studies, which reveal long-range conformational interactions and allosteric links between the V and C regions. This review comprehensively summarizes the existing studies on antibody allostery, including allosteric conformational changes induced by covalent modifications or noncovalent ligand binding.

Development of an external standard method for the high-throughput determination of broadly polar multiclass synthetic dyes in high-sugar, high-fat, and high-protein foods.

Simultaneous detection of multiple synthetic dyes in food additives presents a challenge. A high-throughput UPLC-Q-OMS method with purification-free extraction was developed for the simultaneous detection of 30 synthetic dyes across six categories in various foods. The results showed satisfactory separation of the dyes was achieved using a phenyl column, acetonitrile, and a 5 mmol/L ammonium acetate mobile phase.

A high affinity and selective DNA aptamer for copper ions.

Capture-SELEX was employed for the selection of DNA aptamers for Cu. The best aptamer named Cu-1 has a of 14.2 nM as determined using the strand-displacement assay, representing an approximate 3000-fold improvement over a previously reported Cu aptamer.

Ganoderma lucidum triterpenoids investigating their role in medicinal applications and genomic protection.

Objectives: Ganoderma lucidum (GL) is a white rot fungus widely used for its pharmacological properties and health benefits. GL consists of several biological components, including polysaccharides, sterols, and triterpenoids. Triterpenoids are often found in GL in the form of lanostane-type triterpenoids with quadrilateral carbon structures.

Preparation of a V-COF@SWCNTs-COOH/SPCE supported molecularly imprinted electrochemical sensor for real-time detection of trace sulfadimidine.

To overcome the limitations of insufficient sensitivity and poor specificity of portable screen-printed carbon electrode-electrochemical sensors (SPCE-EC) in practical applications, we prepared carrier composites of carboxylic single-walled carbon nanotubes vertically grafted by covalent organic frameworks (v-COF@SWCNTs-COOH) and coated with a molecularly imprinted polymer (MIP) of sulfadimidine (SM). 55 °C hot steam elution is more eco-friendly than traditional organic solvent elution. The results showed that when the mass ratio of DBA to DBA-SWCNTs was 1:1, the v-COF@SWCNTs-COOH obtained by the two-step synthesis method could increase the electrical signal up to 2.

Unraveling the Metabolomics Mysteries in Camellia Oil: From Cognition to Application.

Camellia oil is a high-value edible seed oil, recommended by the Food and Agriculture Organization (FAO). It is essential to develop accurate and rapid analytical methods to authenticate camellia oil due to its susceptibility to adulteration. Recently, hyphenated chromatography-mass spectrometry, especially high-resolution mass spectrometry using chemometrics, has become a promising platform for the identification of camellia oil.

Rapid screening of prednisolone acetate adulterants in health foods using colloidal gold immunochromatographic assay.

In this study, a rapid detection method utilizing colloidal gold immunochromatography (CG-ICA) was developed for the detection of illegally added prednisone acetate in health foods. Initially, the preparation conditions of colloidal gold solution were optimized. The optimal potassium carbonate dosage, antibody diluent type, antibody dosage, probe labeling time, blocking time and BSA dosage were determined.

Post-synthetic modification fluorescence UiO-66-Eu immunochromatography for high-performance detection of sodium pentachlorophenoate.

Sodium pentachlorophenate (PCP) is widely used as a herbicide, fungicide, or molluscicide. It is highly toxic, easily soluble in water, making it highly prone to diffusion and causing water and soil pollution. Through the food chain, it enters animal bodies and remains in food, causing toxicity to humans and animals.

DNA Barcoding Unveils Novel Discoveries in Authenticating High-Value Snow Lotus Seed Food Products.

Snow Lotus Seed (SLS), esteemed for its nutritional and market value, faces challenges of authentication due to the absence of appropriate testing standards and methods. This results in frequent adulteration of SLS sourced from () with other plant seeds endosperm. Traditional chloroplast DNA barcoding methods are inadequate for species identification due to the absence of chloroplasts in seeds endosperm.

Impact of wheat processing on growth, serum biochemistry, and ruminal microbiota in sheep (Ovis aries).

This study investigated the impact of wheat processing methods (wheat flour vs wheat pellets) on the growth performance, serum biochemical parameters, and rumen microbiome composition in sheep. Results indicated that feeding of wheat flour resulted in significantly higher terminal weight and average daily gain (P < 0.05) and lower cholesterol and ALP04 levels (P < 0.

Nanoflower Microreactor Based Versatile Enhancer for Recognition Cofactor-Dependent Enzyme Biocatalysis toward Saxitoxin Detection.

Investigating organic carriers' utilization efficiency and bioactivity within organic-inorganic hybrid nanoflowers is critical to constructing sensitive immunosensors. Nevertheless, the sensitivity of immunosensors is interactively regulated by different classes of biomolecules such as antibodies and enzymes. In this work, we introduced a new alkaline phosphatase-antibody-CaHPO hybrid nanoflowers (AAHNFs) microreactor based colorimetric immunoprobe.

Serum HMGB-1 released by ferroptosis and necroptosis as a novel potential biomarker for systemic lupus erythematosus.

High mobility group box proterin-1 (HMGB-1) is a multifunctional protein that can be released by various programmed cell deaths (PCDs), such as necroptosis and ferroptosis. PCDs play a critical role in the pathogenesis of systemic lupus erythematosus (SLE). However, the role of HMGB-1 in the process of SLE remains unclear.

The effect of pH shifting on the calcium-fortified milk analogue with chickpea protein.

Milk alternative attracts more attention due to nutrition benefits, but the low solubility and the calcium deficiency of plant protein hinder the development of milk alternatives. Therefore, pH shifting was optimized to improve chickpea protein solubility and calcium fortification while ensuring good digestibility. The results showed that pH shifting reduced the particle size from 2197.

Rapid Screening of Emergent Febuxostat Adulteration in Functional Foods Based on a Simulation-Inspired High-Quality Antibody.

Due to the potential health risks of adulterated febuxostat in uric-acid-lowering foods, it is urgent to develop rapid detection methods. However, there are no fast analytical techniques for febuxostat yet. Herein, an efficient hapten simulation strategy was proposed to successfully produce a highly sensitive and selective monoclonal antibody toward febuxostat.

A Dual and Rapid RPA-CRISPR/Cas12a Method for Simultaneous Detection of Cattle and Soybean-Derived Adulteration in Goat Milk Powder.

The adulteration of goat milk powder occurs frequently; cattle-derived and soybean-derived ingredients are common adulterants in goat milk powder. However, simultaneously and rapidly detecting cattle-derived and soybean-derived components is still a challenge. An efficient, high-throughput screening method for adulteration detection is needed.

Antibody Production and Immunoassay Development for Authenticating Chlorpheniramine Maleate Adulteration in Herbal Tea.

Chlorphenamine maleate is a prohibited additive found in herbal teas and health foods. Excessive intake of this substance can result in adverse health effects. In this study, two novel haptens, PEM and bepotastine (PB1), mimicking chlorphenamine maleate structure were designed and synthesized based on molecular simulation for developing two corresponding polyclonal antibodies (PEM-Ab and PB1-Ab), respectively.