[Effect of poloxamer 407 on the cochlear morphology and hearing function after perfusion in round window: experiment with guinea pigs].

Objective: To observe the effect of sustained-release vehicle-Poloxamer 407 on the function and morphology of the cochlear after perfusion in round window.

Methods: The right ears of 16 guinea pigs as experimental group were perfusion with 20% Poloxamer 407 solusion 100 microl in round window, the left ears as control group with normal saline. Another 4 animals without treatment were in negative control group.

[Effect of poloxamer 407 on the middle ear and inner ear after regional perfusion in guinea pigs].

Objective: To observe the biodegradation and absorption of poloxamer 407 in vivo, and the effect of poloxamer 407 on the middle ear and inner ear after regional perfusion in guinea pigs.

Methods: The right ears of 10 guinea pigs as experimental group were perfused with 20% poloxamer 407 in situ gel 100 microl in round window niche, with the left ears as control group with normal saline. Another two animals without treatment were in negative control group.

[Experimental study of the facial nerve paralysis induced by herpes simplex virus type 1 infection in mice].

Objective: To establish an animal model of Bell palsy induced by type I herpes simplex virus (HSV-1) infection and to assess the role and site of HSV-1 in the pathogenesis of facial paralysis.

Methods: Fifty-three female Balb/c mice four-week-old weighted 16-18 g were studied. After scratching the surface of bilateral auricles with a 26-gauge needle, 25 microL HSV-1 with a titer of 6.