Synaptosomal Actin Dynamics in the Developmental Visual Cortex Regulate Behavioral Visual Acuity in Rats.

Purpose: Synaptosomal actin dynamics are essential for synaptic structural stability. Whether actin dynamics are involved in structural and functional synaptic plasticity within the primary visual cortex (V1) or behavioral visual acuity in rats has still not been thoroughly investigated.

Methods: Synaptosome preparation and western blot analysis were used to analyze synaptosomal actin dynamics.

gene expression and its methylation level in colorectal cancer tissues.

The aim of the present study was to investigate the gene expression and its methylation level in colorectal cancer tissues, as well as the association with colorectal cancer clinical data. In total, 54 cases of colorectal cancer tissue samples and normal adjacent tissue samples were collected between June, 2013 and July, 2014. RT-PCR and western blot analysis were used to detect the mRNA and protein levels of PAQR3 in colorectal samples, respectively.

Zinc oxide nanoparticles inhibit expression of manganese superoxide dismutase via amplification of oxidative stress, in murine photoreceptor cells.

Objectives: As a parenchymal cell, the photoreceptor is more susceptible to alterations in outer micro-environmental conditions than other cells. In the present study, we aimed to investigate inhibitory effects of zinc oxide (ZnO) nanoparticles on expression of manganese superoxide dismutase (MnSOD) in murine photoreceptor-derived cells.

Materials And Methods: We investigated effects of ZnO nanoparticles on murine photoreceptor cell viability and on expression and activity of MnSOD using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, immunofluorescence analysis, flow cytometry, quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA).

[Retrospective investigation and analysis of endemic situation of schistosomiasis in hilly regions of Sichuan Province].

Objective: To explore the rules of the changes of schistosomiasis infection and Oncomelania snails in areas where schistosomiasis transmission was controlled or interrupted or rebounded after interruption.

Methods: Qingbaijiang District, Longquanyi District and Xide County were selected and investigated retrospectively to collect schistosomiasis epidemiological information 10 years before they reached the criterion of transmission control and the subsequent years until 2008. The database of retrospective investigation was established for analyzing the trends and rules of changes of snail status and human infection status.

[Inhibition of integrin beta1 expression in human keratinocyte stem cells by vector-1 based interfering RNA strategy].

Objective: To investigate the influence of integrin beta1 on the proliferation and differentiation of human keratinocyte stem cells (KSCs).

Methods: DNA oligonucleotides targeting integrin beta1 at different locations were synthesized and inserted into BamHI-1 HindIII linearized p Silencer 3.1/H1 plasmids.