[Effect of Luteolin and its combination with chemotherapeutic drugs on cytotoxicity of cancer cells].

Objective: To investigate the effect of Luteolin alone or combination with chemotherapentic drugs on the cytoxicity of cancer cells.

Methods: Cultured A549, Hela, MCF-7, AGS, MGC-803, Caco2 and HepG2 cells were treated with Luteolin or the combination of Luteolin with other chemotherapeutic agents (Bexarotene, Cisplatin and Bleomycin). Cell viability was measured by MTS assay and IC(50) was calculated.

[Nrf2 down-regulated cell line H460-N5 with Keap1 over-expression increased sensitivity to anti-cancer drugs].

Objective: To maked a Nrf2 down-regulated cell line by over-expressing Keap1 in H460 cells to study the role of Nrf2 in drug resistance.

Methods: Transfecting H460 cells with mKeap1-pEGFP and screenig for Keap1 expressing clones by Western blotting with antibodies against Nrf2, HO-1, NQO1 and AKR1C. The cell line with Keap1 over-expression was further confirmed by real-time PCR.

[Clinicopathological significance of cytotoxic lymphocytes in breast cancer and draining lymph nodes].

Objective: To analyze retrospectively the quantity and activation status of the tumor infiltrating cytotoxic lymphocytes in breast cancer and the draining lymph nodes, and its relation to the clinical pathological significance.

Methods: Seventy-four breast cancer samples with their corresponding axillary lymph nodes were histologically typed and staged. Cytotxic lymphocytes were analyzed by immunohistochemistry with the monoclonal antibodies against CD8, CD56, granzyme B and perforin.

[Noninvasive estimation of the left ventricular filling pressure by E/Em during exercise in the early stage of acute myocardial infarction].

Objective: To validate a Doppler index of diastolic filling (E/Em) during exercise in the early stage of the patients with acute myocardial infarction,and investigate its association with exercise capacity.

Methods: The study enrolled consecutive 60 patients with first ST segment elevation myocardial infarction(STEMI)in Killip I-II stage and who underwent percutaneous coronary intervention (PCI) from March 2008 to August 2008(as study group), At 10-14 days after acute myocardial infarction, Cardiopulmonary Exercising Testing (CPET, Modified Bruce flat treadmill protocol, symptom-limited flat exercise tolerance test) was conducted with Tissue Doppler Imaging (TDI) evaluation at rest and instantly during exercising peak. Doppler index of diastolic filling was also measured at rest and immediately after Cardiopulmonary Exercising Testing in 30 age-matched patients as control whose coronary angiography (CAG) or coronary computed tomography was negative.

Correlations of the stability, static dipole polarizabilities, and electronic properties of yttrium clusters.

Static dipole polarizabilities for the ground-state geometries of yttrium clusters (Yn, n < or = 15) are investigated by using the numerically finite field method in the framework of density functional theory. The structural size dependence of electronic properties, such as the highest occupied molecular orbital-lowest occupied molecular orbital (HOMO-LUMO) gap, ionization energy, electron affinity, chemical hardness and softness, etc., has been determined for yttrium clusters.

[Heart-type fatty acid-binding protein alteration in rat's cardiocyte after acute myocardial infarction].

Aim: To explore the changes of mRNA and protein expressions of heart-type fatty acid binding protein (H-FABP) in rat ischemic myocardium at different intervals ischemia.

Methods: 60 SD male rats weighing 250-350 g, were randomly divided into one sham-operated group and five study groups (group A1, A2, A3, A4, A5, the left coronary artery of rats has been ligated for 1 h, 2 h, 4 h, 6 h, 12 h respectively). Myocardil samples from infarct zone, ischemic and non-ischemic zone, were obtained for histology examination, and the mRNA for H-FABP in ischemic myocardial tissue were determined by RT-PCR.

A novel mutation (C1425Y) in the FBN2 gene in a father and son with congenital contractural arachnodactyly.

Congenital contractural arachnodactyly (Beals syndrome) is a rare autosomal dominantly inherited connective tissue disorder characterized by flexion contractures, arachnodactyly, crumpled ears, and mild muscular hypoplasia. Here, a father and son with congenital contractural arachnodactyly features were identified. After sequencing 15 exons (22 to 36) of the FBN2 gene, a novel mutation (C1425Y) was found in exon 33.

Fluorophenyl-substituted Fe-only hydrogenases active site ADT models: different electrocatalytic process for proton reduction in HOAc and HBF4/Et2O.

A set of fluorophenyl-substituted adt-bridged Fe2S2 active site models of Fe-only hydrogenase, [(micro-SCH2)2NR]Fe2(CO)6 (, R=C6F4CF3-p; , R=C6H4CF3-p) and [(micro-SCH2)2NR]Fe2(CO)5(PPh3) (, R=C6F4CF3-p; , R=C6H4CF3-p), have been synthesized and well characterized. Spectroscopic and electrochemical studies demonstrate that the aryl-substituted complexes are stable toward a strong acid HBF4/Et2O, and electrocatalytic process for the hydrogen production is mostly dependent on the strength of the available proton source. When CH3COOH is used as the proton source, the electrocatalytic process begins with successively two one-electron reduction processes to produce H2 at Fe(0)Fe(0) (E2pc); whereas in the presence of strong acid, HBF4/Et2O, the process is initiated by protonation of a N-bridged atom followed by reduction of the protonated N-bridged atom around -1.

[Determination of trace amounts of aluminum by light-absorption ratio variation combined with continuous flow analysis].

In a pH 5.66 acetate-acetic acid medium, the complex reaction of Al(II) with chlorophosphonazo-mS (MSCPA) is very sensitive. In order to react with Al(III) completely, MSCPA must be added excessively enough.

[Comparison of sustainable development status in Heilongjiang Province based on traditional ecological footprint method and emergy ecological footprint method].

By using traditional ecological footprint method and its modification, emergy ecological footprint method, the sustainable development status of Heilongjiang Province in 2005 was analyzed. The results showed that the ecological deficits of Heilongjiang Province in 2005 based on emergy and conventional ecological footprint methods were 1.919 and 0.

Complete genomic sequence analyses of Turnip mosaic virus basal-BR isolates from China.

Isolates of Turnip mosaic virus (TuMV) are divided into four molecular lineages based on host range and geographical origins. Basal-BR is one of the four lineages and represented a new emergent lineage in East Asia. In one previous paper, we report the occurrence of basal-BR isolates in China.

Synthesis, crystal structure and quantum chemical study on 3-phenylamino-4-phenyl-1,2,4-triazole-5-thione.

3-Phenylamino-4-phenyl-1,2,4-triazole-5-thione was synthesized and characterized by elemental analysis, IR and X-ray single crystal diffraction. Density functional theory calculations of the structure, natural bond orbitals, atomic charge distributions and thermodynamic functions of the title compound were performed at B3LYP/ 6-311G** and PBE1PBE/6-311G**levels of theory, respectively. NPA atomic charge distributions indicate that the title compound can be used as a potential multi-dentate ligand to coordinate with various metallic ions.

Psoriasis genome-wide association study identifies susceptibility variants within LCE gene cluster at 1q21.

We report the first large genome-wide association study (GWAS) in a Chinese population to identify susceptibility variants for psoriasis using a two-stage case-control design. In the first stage, we carried out a genome-wide association analysis in 1,139 cases and 1,132 controls of Chinese Han ancestry using Illumina Human 610-Quad BeadChips. In the second stage, we took top SNPs forward for replication in two independent samples of 5,182 cases and 6,516 controls of Chinese Han ancestry, and 539 cases and 824 controls of Chinese Uygur ancestry.

[Oxidative stress and endothelial dysfunction at different serum uric acid levels].

Objective: To investigate the state of oxidative stress in the subjects with different levels of serum uric acid and to explore the cause of endothelial dysfunction induced by hyperuricemia.

Methods: Male subjects with normal serum uric acid or hyperuricemia were enrolled in this study. According to the levels of serum uric acid, all the participants were divided into five groups.

[Screening and analysis of multi-alleles in generalized aggressive periodontitis].

Objective: To detect the susceptible alleles in generalized aggressive periodontitis patients and healthy controls and to analyze the effect of multi-alleles on the occurrence and development of generalized aggressive periodontitis.

Methods: Polymerase chain reaction and cleavage were used for detecting the frequencies of five susceptible genetic polymorphisms in generalized aggressive periodontitis patients and healthy controls. The results were analyzed by Z-score test and mean square analysis.

Preparation of calcium oxalate-bromopyrogallol red inclusion sorbent and application to treatment of cationic dye and heavy metal wastewaters.

Background, Aim, And Scope: Dye pollutants are a major class of environmental contaminants. Over 100,000 dyes have been synthesized worldwide and more than 700,000 tons are produced annually and over 5% are discharged into aquatic environments. The adsorption or sorption is one of the most efficient methods to remove dye and heavy metal pollutants from wastewater.

[The relativity between chronic periodontitis and the genetic polymorphisms of vitamin D receptor and estrogen receptor].

Objective: To investigate the relationship between chronic periodontitis and the genetic polymorphisms of vitamin D receptor gene and estrogen receptor gene.

Methods: Clinical parameters including probing depth, clinical attachment loss, sulcus bleeding index and tooth movement were measured by fluoride probe. Genomic DNA from peripheral venous blood was extracted with saturant sodium chloride, and PCR-restriction fragment length polymorphism was applied to examine the Apa I, Bsm I, Taq I polymorphisms of the vitamin D receptor genes and the Xba I and Pvu II polymorphisms of the estrogen receptor genes.

Facile synthesis and functionality-dependent electrochemistry of Fe-only hydrogenase mimics.

A series of azadithiolate (adt)-bridged Fe-only hydrogenase model complexes, Fe2(CO)6(mu-adt)C6H4I-4 (1), Fe2(CO)6(mu-adt)C6H4C[triple bond]CR [R = C6H4NO2-4 (2), C6H4CHO-4 (3), C6H4NH2-4 (4), C6H4COOH-4 (5), C6H4COOCH2CH3-4 (6), C6H4F-4 (7), C6H5 (8), C6H4OCH3-4 (9), C6H4N(CH3)2-4 (10)], [Fe2(CO)5(PPh3)(mu-adt)C6H4I-4 (11), and Fe2(CO)5(PPh3)(mu-adt)C6H4C[triple bond]CC6H4NO2-4 (12), have been synthesized in high yields under mild conditions. The linear geometry and rigidity of a triple bond act as an effective bridge to anchor a functionality ranging from electron-donating to electron-accepting, even coordinative groups in the adt model complexes. X-ray crystal analysis of 2, 3, and 6-12 reveals that the model complexes retain the butterfly structure of Fe2S2 model analogues.

Brain glucose metabolic changes associated with chronic spontaneous pain due to brachial plexus avulsion: a preliminary positron emission tomography study.

Background: Previous brain imaging studies suggested that the brain activity underlying the perception of chronic pain may differ from that underlying acute pain. To investigate the brain regions involved in chronic spontaneous pain due to brachial plexus avulsion (BPA), fluorine-(18)fluorodeoxyglucose ((18)F-FDG) positron emission tomography (PET) scanning was applied to determine the glucose metabolic changes in patients with pain due to BPA.

Methods: Six right-handed patients with chronic spontaneous pain due to left-BPA and twelve right-handed age- and sex-matched healthy control subjects participated in the (18)F-FDG PET study.

[Study on FTIR spectra of finger nails of normal people and patients of esophagus cancer].

To investigate the application of human finger nails in the diagnosis of cancer, Fourier transform infrared (FTIR) spectroscopy was employed to study the finger nails from some normal people and some with esophagus cancer and others with an operation for curing esophagus cancer five months ago. The results showed that there are obvious differences between FTIR spectra in them in spectral parameters such as frequency, intensity and band shape etc. The changes in the phosphate symmetric stretching vibration v(s) (PO2-) and asymmetric stretching vibration v(a)s(PO2-) modes are uniform, the v(s) (PO2-) and v(a)s(PO2-) absorption peaks of cancerous ones shift to high wave number compared with those of normal people, while those with operation shift to low wave number compared with those of cancerous ones.

The complete genomic sequence of Tobacco vein banding mosaic virus and its similarities with other potyviruses.

The complete genomic sequence of an isolate of Tobacco vein banding mosaic virus (TVBMV-YND) from Yunnan, China was determined by sequencing overlapping cDNA fragments obtained by RT-PCR with degenerate and/or specific primers. The genome is composed of 9,570 nucleotides (nt) excluding the 3'-terminal poly (A) tail and contains one single open reading frame of 9,240 nt encoding a large polyprotein of 3,079 amino acids with predicted Mr of 348.6 kDa.

[Ultrasonographic value for the complications of breast augmentation with injectable polyacrylamide hydrogel technique].

Objective: To analyze ultrasonographic features for the complications of breast augmentation with polyamide hydrogel injection.

Methods: Clinical study was made for 101 patients who had received the polyamide hydrogel injection for augmentation mammoplasty by using the ultrasonographic examination.

Results: The 195 treated breasts in 98 patients suffered from injectable layer disorder, 1 breast for hematoma, 7 breasts in 5 patients for abscess (infection), 2 breasts in 2 patients for cancer and 3 breasts in 3 patients for fibroadenomas.