[Effect of puerarin on the expression of NMDA receptor in the hippocampus CA1 region after focal cerebral ischemia in rats].

Objective: To study the antagonistic action of Puerarin against the excitatory amino acid toxicity, and to further explore its brain protection mechanisms.

Methods: Focal cerebral ischemia model was set up with middle cerebral artery occlusion by intraluminal block in this study. After cerebral ischemia, Puerarin was administered at different time point.

[Protective effect of puerarin against calcium overload after focal cerebral ischemia injury in rats].

Objective: To observe the temporal and spatial changes in the distribution of Ca2+ in the rat brain following focal cerebral ischemia injury and explore the protective effect of puerarin against calcium overload.

Methods: Focal cerebral ischemia was induced by middle cerebral artery occlusion in rats. After cerebral ischemia, puerarin was administered in the rats at different time points.

[Expression of hypoxia inducible factor-1alpha in different brain regions in aged rats].

Objective: To observe the distribution of hypoxia inducible factor-1alpha (HIF-1alpha) in different brain regions in aged rats and investigate the role of HIF-1alpha in the aging process of the nervous system.

Methods: The Nissl bodies and HIF-1alpha expression in different brain regions were observed in rats aged 3 and 30 months using Nissl staining and immunohistochemical method, respectively.

Results: In the 30-month-old rats, the neural cells in 4 different brain regions presented with large cell body and loose alignment, containing reduced Nissl bodies in the cytoplasm.