[Relationship between the long type PML-RAR alpha and the prognosis of patients with acute promyelocytic leukemia].

Objective: To explore the relationship between long (L) type PML-RAR alpha fusion gene and the prognosis of patients with acute promyelocytic leukemia (APL).

Methods: PML-RAR alpha fusion gene was detected by RT-PCR in 33 APL patients. The optical density of three bands including E5 (+) E6 (+) 636 bp (no deletion), E5 (-) E6 (+) 492 bp (exon 5 deleted) and E5 (-) E6 (-) 232 bp (both exon 5 and exon 6 deleted) was measured by a UVP analysis system and their relative proportions were calculated.

Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription.

In this study, an insulinoma-associated antigen-1 (INSM1)-binding site in the proximal promoter sequence of the insulin gene was identified. The co-transfection of INSM1 with rat insulin I/II promoter-driven reporter genes exhibited a 40-50% inhibitory effect on the reporter activity. Mutational experiments were performed by introducing a substitution, GG to AT, into the INSM1 core binding site of the rat insulin I/II promoters.

Possible arrhythmiogenic mechanism produced by ibuprofen.

Aim: The aim of the present study was to investigate the electrophysiological effect of ibuprofen on the cardiac action potentials (AP) and electrocardiograms (ECG), and to identify its arrhythmiogenic mechanism.

Methods: The intracellular microelectrode recording technique was employed to record the fast- and slowresponse AP in guinea pig papillary muscles. The cardiac responses of ibuprofen were monitored by ECG, both in in vivo and in vitro studies.

[Role of progesterone in acylation stimulating protein-receptor C5L2 pathway in adipocytes and preadipocytes].

Objective: To evaluate the effects of progesterone on the mRNA expression of acylation stimulating protein (ASP)-receptor C5L2 in adipocytes and preadipocytes and the C5L2 protein expression on the cell surface.

Methods: Preadipocytes of the line 3T3-L1 were cultured and induced to differentiate. Progesterone of the doses 0 - 1 x 10(-6) mol/L was added into the cultured fluid of the mature 3T3-L1 adipocytes and preadipocytes overnight.

[A multicenter study on treatment of autonomous nerve-mediated syncope in children with beta-receptor blocker].

Objective: Syncope is a common pediatric emergency. Based on an epidemiologic survey in the USA, around 15% of children experienced syncopal attack, which strongly influenced the life, study and hurt the children mentally and physiologically. Therefore, exploring the therapeutic regimen has become a hot topic in the field of pediatric cardiology.

[Flt3 ligand in recovering the function of the splenic dendritic cells and the immune system in mice with multiple organ dysfunction syndrome].

Objective: To study the immunoprotective effect of fms-like tyrosine kinase receptor 3 ligand (FL) in multiple organ dysfunction syndrome(MODS) in mice.

Methods: Ninety mice were randomized into three groups: normal, MODS and MODS+FL (each n=30). The MODS model of mice was reproduced.

2-(4-Chloro-phen-yl)-5-{3,4-dibut-oxy-5-[5-(4-chloro-phen-yl)-1,3,4-oxadiazol-2-yl]thio-phen-2-yl}-1,3,4-oxadiazole.

In the title compound, C(28)H(26)Cl(2)N(4)O(4)S, the dihedral angles between the two chloro-phenyl rings and the two oxadiazol rings are 10.51 (4)° and 13.55 (3)°, respectively.

1-Methyl-1H-indazole-3-carboxylic acid.

The asymmetric unit of the title compound, C(9)H(8)N(2)O(2), contains two mol-ecules. In the crystal structure, both mol-ecules form inversion dimers via pairs of O-H⋯O hydrogen bonds, and a C-H⋯O inter-ation is also seen.

[The effect of arsenic trioxide (As2O3) combined with BSO on K562/ADM cell and its mechanisms].

Objective: To investigate the apoptosis-induction, P-glycoprotein (P-gp) and mdr1 mRNA inhibition effects of arsenic trioxide (As2O3) and buthionine sulfoximine (BSO) on multidrug-resistant cell line K562/ADM cells, and to determine the relationship between intracellular GSH content and arsenic effect.

Methods: K562/ADM cells were treated with arsenic (0.5, 2.

[Expression of acylation stimulating protein receptor (C5L2) in preadipocytes during differentiation and under stimulation of free fatty acids].

Objective: To observe the changes of expression of the acylation stimulating protein (ASP) functional receptor C5L2 during adipocytes differentiation, and to study the regulation of C5L2 by free fatty acid (FFA) in cultured adipocytes and preadipocytes.

Methods: Preadipocytes of the line 3T3-L1 were cultured and induced to differentiate. Oleate or palmitate of the doses 0 - 1.

[Expression of anti-HIV envelope glycoprotein scFv in Pichia pastoris and its biologic activities analysis].

Aim: To construct the vector and express anti-HIV-1 envelope glycoprotein single chain Fv fragment in Pichia pastoris.

Methods: The target gene was digested from plasmid pET28-scFv and cloned into pichia pastoris vector via gene engineering and DNA recombination techniques. The recombinant plasmid was linearized and transferred into Pichia pastoris strains GS115 by electroporation.

Palmitate and oleate induction of acylation stimulating protein resistance in 3T3-L1 adipocytes and preadipocytes.

Acylation stimulating protein (ASP) stimulates triglyceride synthesis and glucose transport via its receptor C5L2. The aims were (i) to evaluate ASP response under insulin-resistant conditions and (ii) to identify mechanisms of ASP resistance using 3T3-L1 adipocytes and preadipocytes. Overnight incubation with palmitate (PAL) or oleate (OLE) induced dose-dependent inhibition of ASP-stimulated glucose transport in adipocytes (198 +/- 18% +ASP, 100 +/- 4% basal, 131 +/- 14% + ASP + 1 mmol/L PAL) and preadipocytes (287 +/- 21% + ASP, 100 +/- 4% basal, 109 +/- 13% + ASP + 1 mmol/L PAL).

[Diagnosis and radiotherapy of germinomas arising in basal ganglia and thalamus].

Objective: To summarize the clinical characteristic and radiotherapeutic effect of germinomas arising in basal ganglia and thalamus.

Methods: The clinical data of 13 cases were reviewed retrospectively. All cases were pathologic diagnosis through stereotactic biopsy.

[Change and role of liver interstitial dendritic cells of mice in a model of multiple organ dysfunction syndrome].

Objective: To explore the role of liver interstitial dendritic cells in immuno-dissonance mechanism in multiple organ dysfunction syndrome (MODS).

Methods: The model of MODS was replicated by intraperitoneal zymosan injection in C57BL/6 mice. One hundred and fifty mice were randomly divided into groups of normal, 3-6 hours, 12-48 hours, 5-7 days and 10-12 days after zymosan injection.

Architecture of the yeast Rrp44 exosome complex suggests routes of RNA recruitment for 3' end processing.

The eukaryotic core exosome (CE) is a conserved nine-subunit protein complex important for 3' end trimming and degradation of RNA. In yeast, the Rrp44 protein constitutively associates with the CE and provides the sole source of processive 3'-to-5' exoribonuclease activity. Here we present EM reconstructions of the core and Rrp44-bound exosome complexes.

[Expression of WT1 gene and its isomer ratio changes during phorbol ester induced differentiation of K562 cell line].

Objective: To explore the changes in expression of WT1 gene and ration of its isomers during phorbol ester (TPA) induced differentiation of leukemia cell line K562 by fluorescence quantitative RT-PCR and analysis the relationship between different isomers and hematogenic cell differentiation.

Methods: The degree of cellular maturation were verified by NBT reduction test and immunophenotyping. Expression of WT1 gene was determined by fluorescence quantitative RT-PCR during differentiation of K562 cell line.

Childhood gynecomastia: a clinical analysis of 240 cases.

Two hundred forty cases of childhood gynecomastia were studied retrospectively. There were 13 cases aged 3 to 10 years and 227 cases aged 11 to 15 years. Of the 240 cases of gynecomastia, 160 presented with bilateral breast enlargement, 50, left breast enlargement, and 30, right breast enlargement.

Study of protoporphyrin IX (PpIX) pharmacokinetics after topical application of 5-aminolevulinic acid in urethral condylomata acuminata.

The pharmacokinetics of 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) in lesions of urethral condylomata acuminata were investigated. Sixty patients (20 to 60 years old, 48 male and 12 female) were divided randomly into five groups and received topic application of different concentrations of ALA solution (0.5%, 1%, 3%, 5% or 10%).

[Expression of fibrillin-1 in congenital bicuspid aortic valves].

Objective: To detect the expression of fibrillin-1 in congenital bicuspid aortic valves, and to investigate the molecular mechanism of congenital bicuspid aortic valves.

Methods: Specimens of aortic valve were obtained from 12 pediatric patients with congenital bicuspid aortic valve, 11 boys and 1 girl, aged 16.7 (10 - 18), including 5 cases of aortic stenosis (AS), 8 of aortic insufficiency (AI), and 1 of AS and AI, undergoing valve replacement, 8 children who died accidentally without cardiovascular system and collagen system diseases, 6 boys and 2 girls, aged 9.

[Implications of lung interstitial dendritic cells of mice in multiple organ dysfunction syndrome].

Objective: To explore the role of lung interstitial dendritic cells in immunodissonance and organ injury in multiple organ dysfunction syndrome (MODS).

Methods: Animal model of MODS was established by injecting zymosan into the peritoneal cavity of C57BL/6 mice. The mice were randomly divided into groups of normal, 3 - 6 hours, 12 - 48 hours, 5 - 7 days, 10 - 12 days post injection.

[Epidemiological study of Streptococcus pneumoniae in the nasopharynx of healthy children under 5 years of age in Wuhan].

Objective: To investigate the population biology of Streptococcus (S.) pneumoniae carried by healthy children under 5 years of age in Wuhan.

Methods: S.

Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms.

The Dam1 kinetochore complex is essential for chromosome segregation in budding yeast. This ten-protein complex self-assembles around microtubules, forming ring-like structures that move with depolymerizing microtubule ends, a mechanism with implications for cellular function. Here we used EM-based single-particle and helical analyses to define the architecture of the Dam1 complex at 30-A resolution and the self-assembly mechanism.