Publications by authors named "Hong-tao Dou"
Article Synopsis
- Candida auris is a multidrug-resistant fungal pathogen causing outbreaks in China, and its resistance mechanisms are not fully understood, especially in strains developed in live hosts.
- The study analyzed 16 strains from two patients using whole-genome sequencing, revealing all belonged to the South Asian lineage with distinct mutations linked to antifungal resistance, particularly in the ERG11 and TAC1b genes.
- The findings indicate a potential transmission of C. auris and highlight the need for further research into the genetic mutations and resistance mechanisms to improve monitoring and treatment strategies.
Objective: To investigate the distribution and antimicrobial susceptibility of causative microorganisms recovered from patients with intra-abdominal infections (IAIs).
Methods: A total of 2,926 bacterial and fungal strains were identified in samples collected from 1,679 patients with IAIs at the Peking Union Medical College Hospital between 2011 and 2021. Pathogenic bacteria and fungi were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Article Synopsis
- A study collected 111 fungal isolates, including 103 Penicillium and 8 Talaromyces, from clinical sources in a Beijing medical center between 2016-2017, confirming identification through genetic sequencing.
- Findings revealed that Penicillium oxalicum was the most common species among Penicillium isolates, while Talaromyces funiculosus was dominant among Talaromyces isolates; both groups exhibited significant growth at 37°C.
- Antifungal tests showed that azoles and amphotericin B were effective against T. marneffei, whereas echinocandins had the lowest minimum effective concentrations (MEC) for most species, highlighting the need for further understanding of
Lentivirus-mediated knockdown of Krüppel-like factor 9 inhibits the growth of ovarian cancer.
Arch Gynecol Obstet
February 2015
Purpose: Ovarian cancer is among the top diseases in the list of malignant gynaecologic tumors. In the present study, we aim to investigate the effect of lentivirus-mediated knockdown of Krüppel-like factor 9 (KLF9) on cell viability and tumor growth in ovarian cancer.
Methods: Firstly, the expression of KLF9 was determined by real-time PCR and western blot in human ovarian cancer tissues.
Three reference and 45 clinical isolates of Trichosporon were analyzed by conventional phenotypic and molecular methods to determine the species and genotypes of Trichosporon isolates from China. Target loci for molecular methods included the internal transcribed spacer (ITS) region, the D1/D2 domain of the 26S rRNA gene, and the intergenic spacer 1 (IGS1) region. Identification of eight Trichosporon species was achieved, of which Trichosporon asahii was the most common.
View Article and Find Full Text PDFEleven reference and 25 clinical isolates of Fusarium were subject to multilocus DNA sequence analysis to determine the species and haplotypes of the fusarial isolates from Beijing and Shandong, China. Seven loci were analyzed: the translation elongation factor 1 alpha gene (EF-1α); the nuclear rRNA internal transcribed spacer (ITS), large subunit (LSU), and intergenic spacer (IGS) regions; the second largest subunit of the RNA polymerase gene (RPB2); the calmodulin gene (CAM); and the mitochondrial small subunit (mtSSU) rRNA gene. We also evaluated an IGS-targeted PCR/reverse line blot (RLB) assay for species/haplotype identification of Fusarium.
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