[Treatment of early avascular necrosis of femoral head by core decompression and transplantation of human hepatic growth factor gene-modified osteoblasts: experiment with rabbits].

Objective: To evaluate the effects of transplantation of human hepatocyte growth factor (hHGF) gene-modified osteoblasts combined with core decompression in treatment of avascular necrosis of femoral head (ANFH).

Methods: The plasmid pcDNA3.1(+)-hHGF containing hHGF gene was constructed.

[Biocompatibility of combined deproteinized bone coated with hepatocyte growth factor as scaffold for osteoblasts in vitro in fetal rabbits].

Objective: To determine the cellular compatibility of combined deproteinized bone(DPB) coated with hepatocyte growth factor (HGF), and to observe the adherent effect of osteoblasts in response to HGF.

Methods: Osteoblasts were isolated from fetal rabbits. Osteoblasts were cultured with DPB coated with HGF and deproteinized bone as experimental group and contral group, respectively.

[Construction and expression of recombinant plasmid pcDNA3.1(+) -hHGF available in osteoblast].

Objective: To construct a plasmid carrying human hepatocyte growth factor (hHGF) gene and determine the effects of the hepatocyte growth factor (HGF) gene on proliferation and differentiation of osteoblast in vitro.

Methods: The full length cDNA of hHGF, which was amplified from human liver mRNA by RT-PCR, was cloned into pcDNA3.1(+) vector to construct pcDNA3.

[Platelet and endothelial cell-derived microparticles in steroid-induced osteonecrosis of the femoral head of rabbit model].

Objective: To explore the relationship between the alterations of platelet-derived microparticles (PMPs) and endothelial cell-derived microparticles (EMPs) and steroid-induced osteonecrosis of the femoral head.

Methods: Forty-four adult Japanese white rabbits were randomly divided into 2 groups: experimental group (n = 44), injected intramuscularly once with 20 mg/kg of methylprednisolone acetate, and control group (n = 28) injected with normal saline of the same dose. Blood samples were collected from the auricular vein while the animals were in a fasting state in early morning prior to experiment (week 0), and 2, 4, and 8 weeks after the injection.