Publications by authors named "Hong-ning Wang"
Article Synopsis
- Bacterial resistance to β-lactams is largely due to CTX-M-type extended-spectrum β-lactamases (ESBLs), with a study examining various chicken-derived E. coli strains in China to better understand this issue.
- Out of 1808 chicken strains tested between 2012-2020, 222 were identified as carrying the bla-Ec gene, with notable differences in prevalence between chicken (42.7%) and human (26.2%) isolates, with certain sequence types, such as ST155 in chickens and ST152 in humans, being more common.
- The study revealed close genetic relationships in the bla genes, suggesting a potential for plasmid-mediated transfer of resistant genes between chickens and humans,
Article Synopsis
- Escherichia coli (E. coli) is a harmful bacterium that can harbor antimicrobial resistance genes, making it a major concern in both food-borne diseases and clinical infections, particularly if found in retail meat.
- In a study of 464 meat samples in China, 330 E. coli strains were isolated, revealing a wide genetic variety with 146 different sequence types, out of which 20 were newly identified, indicating significant genetic diversity.
- A concerning 82.42% of the E. coli strains were multidrug-resistant, and key resistance genes like mcr-1 and mcr-10 were found, highlighting the potential for these resistance traits to be transferred to humans through contaminated meat.
Objectives: To characterize the genetic environments of ESBL gene blaVEB-1 in mcr-positive Aeromonas strains from raw meat in China.
Methods: Whole genomes of Aeromonas strains were sequenced using the Illumina or Nanopore platforms. Genetic environments of blaVEB-1 were analysed using the BLAST program.
Unlabelled: Infectious bronchitis virus (IBV), the causative agent of infectious bronchitis, is responsible for major economic losses in the poultry industry worldwide. While IBVs can usually be passaged in primary chicken embryonic fibroblasts (CEFs), most of the wild ones cannot adapt to passaged cell lines. In this study, the wild strain CK/CH/MY/2020 was used to infect primary CEF and immortalize DF-1 CEF cells.
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- The study examined the presence of SXT/R391 integrative and conjugative elements (ICEs) in 103 bacterial strains isolated from 25 farms in China, revealing that 25.2% contained these ICEs, which are capable of transferring antibiotic resistance genes (ARGs) to other bacteria.* -
- All SXT/R391 ICE-positive strains exhibited significant levels of drug resistance, and conjugation experiments showed efficient transfer rates of these elements, with one ICE (ICEChnSC1111) carrying 19 different ARGs, the highest reported for an SXT/R391 ICE.* -
- Despite some ICEs inducing a fitness cost in recipient bacteria, long-term exposure to antibiotic pressure may lead to compens
Article Synopsis
- 4,[5],12:i:- is a globally spread variant of Typhimurium that emerged in the late 1980s, predominantly causing infections in animals and humans, particularly in China where its prevalence among swine has increased.
- A study analyzed 54 strains from pigs of different ages, revealing that most belonged to the same genetic group (ST34) and showed diverse genetic markers, indicating a complex evolution and potential multiple origins of the strain in the farm.
- The research highlighted the presence of various antimicrobial resistance genes through plasmids and on chromosomes, emphasizing the need for ongoing monitoring of this drug-resistant bacterium as swine farms play a significant role in its spread to humans.
Extraordinary excitability (hyperexcitability) is closely related to retinal ganglion cell (RGC) injury in glaucoma. Dopamine (DA) and its receptors are involved in modulating RGC excitability. We investigated how DA system affects RGC injury in chronic ocular hypertension (COH) experimental glaucoma model.
View Article and Find Full Text PDFis an opportunistic pathogen frequently associated with nosocomial infection and food poisoning cases. Contamination of in retail meat products may be important transmission routes for human infection with . In this study a total of 89 strains were isolated from 347 samples in 14 food markets in China and subjected to whole-genome sequencing.
View Article and Find Full Text PDFRetinal ganglion cell apoptotic death is the main pathological characteristic of glaucoma, which is the leading cause of irreversible blindness. Disruption of Ca homeostasis plays an important role in glaucoma. Voltage-gated Ca channel blockers have been shown to improve vision in patients with glaucoma.
View Article and Find Full Text PDFPrevious work showed that ephrinA3/EphA4 forward signaling contributed to retinal ganglion cell (RGC) damage in experimental glaucoma. Since up-regulated patterns of ephrinA3 and EphA4 were observed in Müller cells and RGCs, an EphA4/ephrinA3 reverse signaling may exist in Müller cells of chronic ocular hypertension (COH) retina. We investigated effects of EphA4/ephrinA3 reverse signaling activation on Müller cells in COH retina.
View Article and Find Full Text PDFDeficiency of glutamate transporter GLAST in Müller cells may be culpable for excessive extracellular glutamate, which involves in retinal ganglion cell (RGC) damage in glaucoma. We elucidated how GLAST was regulated in rat chronic ocular hypertension (COH) model. Western blot and whole-cell patch-clamp recordings showed that GLAST proteins and GLAST-mediated current densities in Müller cells were downregulated at the early stages of COH.
View Article and Find Full Text PDFAntibiotic resistance genes (ARGs) as a novel type of environmental pollutant pose a health risk to humans. Oxazolidinones are one of the most important antibiotics for the treatment of Gram-positive bacterial infections in humans. Although oxazolidinones are not utilized in the livestock industry, florfenicol is commonly used on farms to treat bacterial infections, which may contribute to the spread of the , , and genes on farms.
View Article and Find Full Text PDFWhole genome sequence of Proteus mirabilis ChSC1905 strain harbouring a new SXT/R391-family ICE.
J Glob Antimicrob Resist
September 2022
Article Synopsis
- The study aimed to analyze the whole genome sequence of a multidrug-resistant Proteus mirabilis strain (ChSC1905) found in a pig farm in China.
- The genome was sequenced using advanced methods, resulting in a large sequence with numerous antimicrobial resistance genes that can spread to other bacteria.
- The findings highlighted a newly identified integrative and conjugative element (ICE) that plays a role in genetic changes, emphasizing the need for further research on such elements in antibiotic resistance.
Article Synopsis
- * The genome analysis revealed SCSW714 has a size of about 4.9 million base pairs and contains 4759 genes, including six genes responsible for antibiotic resistance, grouped in a stable transposon named Tn6777.
- * This research is significant as it documents the complete genome sequence of S. Rissen and identifies a novel transposon that may facilitate the spread of resistance genes from animals to humans.
Article Synopsis
- * The newly isolated recombinant strain CK/CH/MY/2020 from southwest China belongs to the GI-28 genotype and is derived from three live attenuated vaccine strains, featuring complex recombination with at least nine sites involved.
- * Pathogenicity tests revealed that CK/CH/MY/2020 causes respiratory and kidney diseases in chickens, resulting in moderate mortality, indicating it is more virulent than the original vaccine strains, showcasing ongoing evolution of IBV even without wild strain presence. *
Objectives: This study aimed to clarify the characteristics of Tn7-derivatives transposons in MDR Proteus mirabilis strains isolated from anal swabs of chicken and swine in China from 2015-2020.
Methods: The Tn7 tnsA gene was screened in 207 P. mirabilis isolates by polymerase chain reaction (PCR).
Article Synopsis
- * Researchers collected 178 florfenicol-resistant enterococci isolates from healthy pigs in a Chinese farm, finding the poxtA gene in 11 of these samples, which included several different strains of Enterococcus species.
- * Whole genome sequencing revealed that poxtA genes were found on varied plasmids, suggesting that both clonal spread and horizontal gene transfer are key mechanisms for the gene's dissemination among enterococci in the pigs.
Background: Neuroinflammation plays an important role in the pathogenesis of glaucoma. Tumor necrosis factor-alpha (TNF-α) is a major pro-inflammatory cytokine released from activated retinal glial cells in glaucoma. Here, we investigated how TNF-α induces retinal ganglion cell (RGC) hyperexcitability and injury.
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- * In a study involving 74 isolated strains of S. enterica from various stages of broiler chicken production in Sichuan, China, the highest isolation rates were found during defeathering and evisceration, with Mbandaka serotype being the most prevalent.
- * WGS analysis identified multiple sequence types and showed that several serotypes, particularly Mbandaka, Indiana, and Kentucky, could be transmitted within the production chain, highlighting the need for routine surveillance to monitor resistance and ensure food safety.
Article Synopsis
- The study investigates the genome of a New Delhi metallo-β-lactamase 1 (NDM-1) producing Proteus mirabilis strain from broiler chickens in China, highlighting concerns for public health due to antibiotic resistance.
- The genome sequencing revealed a chromosome size of 4,014,504 bp with multiple antimicrobial resistance genes, including those for ESBL and fluoroquinolone resistance.
- The findings provide critical insights into the potential transmission of resistant strains from poultry to humans and the evolution of resistance plasmids, contributing to a better understanding of antibiotic resistance spread.
Article Synopsis
- Incompatibility group C (IncC) plasmids are concerning due to their ability to carry and spread antibiotic resistance genes from animals to humans, posing a public health threat.
- A study characterized 20 IncC plasmids from food-producing animals in China, identifying four as type 1, 15 as type 1/2 hybrids, and one as type 2, further classifying hybrids into subtypes based on genetic differences.
- The research revealed that these plasmids showed a variety of genetic mutations and rearrangements, particularly involving insertion sequences, contributing to their structural diversity and ability to resist multiple antibiotics.
Detection of Mobile Colistin Resistance Gene in a Conjugative Plasmid from of Chicken Origin in China.
Antimicrob Agents Chemother
September 2020
Article Synopsis
- * Researchers sequenced EG81 using advanced technologies, identified multiple antimicrobial resistance genes, and found significant genetic information including a chromosome and two plasmids in its genome.
- * The findings highlight the presence of the optrA resistance gene and suggest that this strain poses a significant public health risk due to its combined resistance to vancomycin and other antibiotics.
Objectives: To characterize the MDR genomic islands (GIs) in Proteus mirabilis isolates.
Methods: Two P. mirabilis strains (C55 and C74) of chicken origin were subjected to WGS (HiSeq and PacBio) and the MDR GIs were determined.
Objectives: To characterize the genetic environment of the carbapenem resistance determinant in Proteus vulgaris of swine origin.
Methods: The carbapenem-resistant P. vulgaris strain BC22 was isolated from a faecal swab from a diseased pig with diarrhoea in Sichuan Province of China in 2018.