Association of Estrogen Receptor 1 Genetic Polymorphisms with Recurrent Spontaneous Abortion Risk.

Background: Estrogen is one of the most important reproductive steroidal hormones and plays a critical role in the maintenance of pregnancy, and its function is mediated by estrogen receptor 1(ESR1). The polymorphisms of ESR1 were involved in recurrent spontaneous abortion (RSA); however, the association between ESR1 polymorphisms and RSA remains controversial. The present meta-analysis was aimed to clarify the association between ESR1 PvuII (-397C/T, rs2234693) and XbaI (-351A/G, rs9340799) polymorphisms and the risk of RSA.

[Construction of rapid tagging vector of genes in Giardia lamblia].

Objective: To construct a recombinant vector for rapid gene tagging in Giardia lamblia.

Methods: To obtain the recombinant vector pGL gdh-Neo with the Neo selection marker, the Neo gene was put under the control of gdh promoter by overlap PCR and inserted into pGEM-5zf. A DNA fragment containing multiple cloning sites (MCS) followed by triple hemagglutinin(3HA) coding sequences was synthesized and cloned into the pGL gdh-Neo to construct a recombinant vector pGL MCS-3HA-gdh-Neo.

Does Traditional Chinese Medicine pattern affect acupoint specific effect? Analysis of data from a multicenter, randomized, controlled trial for primary dysmenorrhea.

Objectives: This study assessed the importance of the Traditional Chinese Medicine (TCM) pattern on an acupoint-specific effect.

Design: This was a TCM pattern subdivision analysis of the first intervention data from a multicenter, randomized, controlled trial (ISRCTN24863192) (the main trial).

Settings: The main trial recruited participants from six hospitals in three provinces in China.

[Transcriptional regulation by silence information regulator 2].

Silence information regulator 2 (Sir2) family is a group of conserved deacetylases, widely existed in organisms from archaea to mammals, and characterized by NAD(+)-dependent deacetylase and ADP-ribosyltransferase activities. Sir2 plays an important role in various life progresses, such as chromatin silence, gene regulation, metabolic regulation, life span of cells and so on. Through its deacetylase activity and/or interaction with other proteins, Sir2 can regulate chromatin structure or modify transcription related factors, thus regulating the transcriptional process.

[Cloning, expression and purification of silent information regulator 2 from Giardia lamblia].

Objective: To clone and express silent information regulator 2 (Sir2) gene from Giardia lamblia.

Methods: The GlSir2 gene was amplified by PCR from genomic DNA of Giardia lamblia (Chinese strain C2 clone). PCR product was cloned into pMD-19T vector and transformed into E coli JM109.