Publications by authors named "Hong-lin Tang"
[Tyrosine kinases inhibitor A77 1726 inhibits upregulation of collagen generation in fibroblasts induced by IL-13].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
January 2009
Article Synopsis
- The study aimed to investigate how the tyrosine kinase inhibitor A77 1726 affects collagen production in fibroblasts stimulated by IL-13.
- The researchers used various methods, including MTT assays, hydroxyproline release assays, RT-PCR, and Western blot, to assess fibroblast proliferation and collagen synthesis after treatment with A77 1726.
- Results showed that A77 1726 significantly inhibited fibroblast growth and collagen production over time, indicating its potential role in reducing collagen synthesis in response to IL-13 stimulation.
[Effects of interleukin 13 on the differentiation and expression of transcription factor c-fos of HEL cells].
Zhonghua Xue Ye Xue Za Zhi
August 2006
Objective: To study the effects of IL-13 on the differentiation and expression of transcription factor c-fos of human erythroleukemia cell line (HEL) cells.
Methods: Reverse transcription polymerase chain reaction (RT-PCR) was used to observe the mRNA expression of IL-13 receptor a 1, GP i b, vWF and c-fos, and Western blot and cytometry were used to analyse their protein expression.
Results: IL-13 receptor a 1 was expressed on HEL cells.
A soluble truncated cadherin induces breast cancer cell apoptosis and growth inhibition.
J Cancer Res Clin Oncol
September 2006
Purpose: To identify the characteristics and function of the truncated cadherin cDNA which encodes a soluble molecule containing the sequence of VE-cadherin extracellular domain repeats from repeat 1 to 4 (designated as CED1-4) and a secreting signal peptide at N terminal.
Methods: A pMSCV/CED1-4 vector was constructed. Recombinant retrovirus ReCED1-4 and ReEmpty were produced by 293 package cells and transfected into MDA-MB435 human breast cancer cells.
[Effects of MAPK antagonist on TPO stimulated UT2 cells proliferation and differentiation].
Zhonghua Xue Ye Xue Za Zhi
May 2005
Objective: To explore the effects of MAPK antagonist on TPO stimulated UT7 cell proliferation and differentiation, and to elucidate the mechanism of TPO functioning on UT7 cells.
Methods: EGFP pMSCV and MEK 1 pMSCV MEK 1 plasmids were transferred into UT7 cells. Phosphorylated MEK1 of UT7 cells was examined by Western blot.
Background: This study was designed to obtain a recombinant retroviral vector containing the human hepatocellular carcinoma-related gene ANGPTL4 (angiopoietin-like 4) cDNA and to evaluate the anti-tumor effect of recombinant retroviral vector-mediated human ANGPTL4 gene transfection.
Methods: ANGPTL4 cDNA was cloned in vitro from normal human liver cells HL-7702 by using RT-PCR, and then subcloned into the plasmid vector pMSCV and sequenced. The retroviral plasmid vectors pMSCV-ANGPTL4, pVSV, and pGAG-POL were co-transfected into the packaging cell line 293 EBNA under mediation of lipofectamine.