Two new dammarane-type triterpenoids from the green walnut husks of Maxim.

Two new dammarane-type triterpenoids, dammar-3,12(),20()-triol-12,32();20,32-diepoxy-25-methy-25-en-tridecacyclic ether () and (23)-12,20(),25(),26-tetrahydroxydammar-23-en-3-one () were isolated from the green walnut husks of Maxim together with six known compounds. Their structures were elucidated through extensive spectroscopic analyses and by comparison with the literature, and the cytotoxic activities of these compounds were evaluated.

A new triterpene from the green walnut husks of Juglans mandshurica Maxim.

A new triterpene named klodorol B (1), together with six known compounds, were isolated from the green walnut husks of Juglans mandshurica Maxim. Their structures were determined using spectroscopic methods on the basis of 1D and 2D NMR, and high-resolution electrospray ionization mass spectrometry. The isolated compounds were evaluated for their cytotoxic activities on human gastric carcinoma (BGC-823), human liver cancer (HepG-2) and human lung cancer (A549) cell lines.

-Tetralone glycosides from the green walnut husks of Maxim. and their cytotoxic activities.

Five new -tetralone glycosides, juglanbiosides A-E (), together with an -tetralone derivative () and nine known 1,4-naphthoquinones () were isolated from the 95% EtOH extract of green walnut husks of Maxim. Their structures were elucidated by comprehensive spectroscopic methods (H, C NMR, DEPT, HSQC, HMBC, CD, HR-ESI-MS). In cytotoxicities of all the isolated compounds were evaluated against BGC-823, HCT-15 and K562 cancer cell lines.

Two new tetralone glycosides from the green walnut husks of Maxim.

Two new tetralone glycosides, 4()-5-methoxy juglanoside A (), 4()-5-methoxy juglanoside D (), together with ten known compounds (-) have been isolated from the green walnut husks of Maxim. Their structures were elucidated on the basis of their ESI-MS, 1 D and 2 D NMR spectroscopic data. In addition, all compounds were evaluated for their cytotoxic activities against the cancer BGC-823 (human gastric carcinoma), HCT-15 (human colorectal carcinoma) and K562 (human chronic myeloid leukemia) cell lines.

The proteomic analysis of human neonatal umbilical cord serum by mass spectrometry.

Aim: To investigate the proteome composition and function of human neonatal arterial umbilical cord.

Methods: Serum proteomic analyses were performed on samples from both males and females by using a combination of techniques: (1) removal of six high-abundance proteins, (2) tryptic digestion of low-abundance proteins, (3) separation of peptide mixtures by reverse-phase high-performance liquid chromatography (RP-HPLC), and (4) peptide identification using electrospray ionization tandem mass spectrometry (ESI-MS/MS).

Results: A total of 837 non-redundant proteins were identified, with 213 male-specific and 239 female-specific proteins.