Reconstruction of the ocular surface by autologous transplantation of rabbit adipose tissue-derived stem cells on amniotic membrane.

Background: Corneal disease is the second most common cause of blindness in China. Clinically, treatment options for corneal diseases with limbal stem cell deficiency (LSCD) are limited due to a shortage of organ donors and inevitable immune rejection. This study aims to determine the efficacy of reconstructing the ocular surface using autologous cultivated adipose tissue-derived stem cells (ADSCs) and to develop a new clinical treatment for patients with LSCD.

Is glaucoma comprehension associated with psychological disturbance and vision-related quality of life for patients with glaucoma? A cross-sectional study.

Objective: To investigate whether understanding of glaucoma is associated with psychological disturbance and vision-related quality of life (QoL) in patients with glaucoma.

Design: A prospective, hospital-based, cross-sectional study.

Setting: Shanghai, China.

[Comparison of manual versus automatic titration in pressure determination for long-term therapy of continuous positive airway pressure in patients with obstructive sleep apnea hypopnea syndrome].

Objective: To compare the continuous positive airway pressure (CPAP) of automatic titration with that of manual titration.

Methods: A total of 58 patients with obstructive sleep apnea and hypopnea syndrome (OSAHS) diagnosed by overnight polysomnography at sleep center of First Affiliated Hospital, Guangzhou Medical University were studied between December 2010 and December 2012. Manual titration was performed under full polysmnography and auto-titration at home for 3-7 nights.

[Morphological characteristics of cornea in patients with vernal keratoconjunctivitis by in vivo laser scanning confocal microscopy].

Objective: To explore the morphological characteristics on cornea in patients with vernal keratoconjunctivitis (VKC) by the application of in vivo laser scanning confocal microscopy (LSCM).

Methods: The experimental design was retrospective observation case series (case control study). Twenty-six patients, each diagnosed as bilateral VKC, were enrolled in the study, among which 13 were tarsal form, 5 were bulbar form and the rest were mixed form.

[Clinical applications and limitations of anterior segment optical coherence tomography].

Optical coherence tomography (OCT) is being employed more and more often to facilitate the diagnosis and management of the anterior eye segment diseases. The cross-sectional imaging capability of its higher resolution allows OCT to measure and visualize clear anatomic structures, specifically in anterior chamber biometry, corneal pachymetric mapping, and anterior chamber angle. Fourier-domain OCT technique which achieves higher speed and higher resolution and time-domain OCT technique have driven their further application in ophthalmological practice.

[Advances in penetrating keratoplasty technique].

Rejection after corneal transplantation is currently the lead of the main reasons for corneal graft failure. Penetrating keratoplasty (PK) is still one of the gold standard surgeries for these patients. In recent years, improving the surgical techniques to reduce the incidence of corneal allograft rejection has been the way to more and more attention to the majority of ophthalmologists.

[The ocular surface of severe alkali burns patients on confocal microscopy].

Objective: To analyze the morphology on the ocular surface of severe alkali burns patients by in vivo laser scanning confocal microscopy.

Methods: This research was a retrospective observation case series. From February to November 2008 in Eye Ear Nose and Throat Hospital of Fudan University, 39 alkali burns patients who classified as III or IV according to Roper-Hall classification were enrolled in this study.

[Expression of regulatory T cells and related cytokines in mice allogenic corneal transplantation rejection].

Objective: To study the changes of CD4(+)CD25(+) and CD8(+)CD103(+) regulatory T cells and their relevant cytokines in corneal allograft transplantation in mice.

Methods: BABL/c (H-2(d)) mice received corneal allografts from C3H/He (H-2(k)) mice were served as the experimental group. BABL/c (H-2(d)) mice received corneal from BABL/c (H-2(d)) mice were used as the control group.

An in vivo confocal microscopy and impression cytology analysis of goblet cells in patients with chemical burns.

Purpose: To evaluate goblet cell density (GCD) on conjunctiva and cornea in patients with ocular chemical burns by in vivo laser scanning confocal microscopy (LSCM) and impression cytology (IC) and to explore the correlation between two methods.

Methods: Fifty-four patients (58 eyes) with chemical burn were enrolled in the study. LSCM was applied to identify the goblet cells on conjunctiva and cornea under in vivo conditions, and GCD was analyzed with the customized software.

[Chromosome karyotype and Y chromosome microdeletion analysis in 133 idiopathic male infertile patients].

Objective: To explore the relationship between chromosomal abnormalities and Y chromosome microdeletions in idiopathic male infertile patients.

Methods: Karyotypic analysis and PCR were carried out in 133 male idiopathic infertile patients for chromosome karyotype and Y chromosome microdeletion analysis.

Results: Of the 133 patients, 25 (18.

[Variations of the microstructure in human limbus by confocal microscopy in vivo].

Objective: To demonstrate the variations in the niche of limbal stem cells and the limbal basal epithelial cells in vivo by confocal microscopy.

Methods: One hundred and twenty eyes of 120 healthy subjects were enrolled in this study after a routine slit-lamp examination. They were divided into four groups according to the age: group A (0-), group B (20-), group C (40-) and group D (60 - 79).

Pediatric penetrating keratoplasty in Shanghai: a retrospective multiple centre study from 2003 to 2007.

Background: With advances in pediatric keratoplasty over the last two decades, the success rate of surgery has increased remarkably. However, few epidemiological studies in this field have been performed in China. The present study investigated the indications and characteristics of pediatric penetrating keratoplasty in Shanghai.

[Roles of angiopoietins family and its receptor in the pathogenesis and regulation of corneal neovascularization].

Corneal neovascularization is one of the tough problems in the cornea diseases, but its pathogenesis is still unclear. Binding of angiopoietins family and its receptor Tie2 set up the signal transducing system of Ang/Tie2, which plays the important role in the angiogenesis and regulation. Recently, the study of the signal transducing system of Ang/Tie2 has archived some progression in the corneal neovascularization.

[The influence of lens and vitreous resection on anterior chamber associated immune deviation in mice model].

Objective: To explore the impact of lens and vitreous resection on the induction of the anterior chamber associated immune deviation (ACAID) in the mice, and its relationship with the time duration postoperatively.

Methods: The current study was an experimental research. Fifty-one Wistar mice were chosen to involve this study.

[Comparative study on deep lamellar keratoplasty and penetrating keratoplasty for keratoconus].

Objective: To investigate the therapeutic outcomes of deep lamellar keratoplasty (DLKP) and penetrating keratoplasty (PKP) for keratoconus.

Methods: Retrospective review of 29 eyes of 29 keratoconus patients, who underwent surgery in Eye & ENT Hospital of Fudan University from April in 2003 to April in 2006. Eleven eyes underwent DLKP and the rest had PKP.

[Clinical analysis of keratoplasty for 121 children in Shanghai].

Objective: To determinate the clinical characteristics of children underwent corneal transplantation in Shanghai, including the indications, the age and gender distributions, etc..

Methods: Records of 122 keratoplasties in 122 eyes of 121 children below 17 years old, undertaken from January 1, 2003 to January 1, 2006, were gathered by four ophthalmic units in Shanghai as a multiple centre study and the data were analyzed by the statistic software SPSS 13.