Understanding ruminal microbiota and diet-host breed interactions under forage feeding conditions is essential for optimizing rumen fermentation and improving feed efficiency in small ruminants. This study aimed to investigate the effects of different ratios of condensed tannin-rich Sericea lespedeza (SL; Lespedeza cuneata) in the diets on changes and interactions of ruminal microbiota and host species (i.e.
View Article and Find Full Text PDFThis study was to evaluate the mitigative effects of vitamin C (VC) on growth inhibition and intestinal damage induced by glycinin in juvenile Rhynchocypris lagowskii Dybowski. 270 healthy juvenile Rhynchocypris lagowskii Dybowski (4.65 ± 0.
View Article and Find Full Text PDFThe present study was implemented to evaluate oxidative stress, immune response, Nrf2 and NF-κB signaling molecules related genes expression of Rhynchocypris lagowski living in biofloc technology (BFT) system and exposed to waterborne ammonia. According to the differences of C:N ratios, the experiment was divided into four groups: C:N 10.8:1 (control group), C:N 15:1, C:N 20: 1 and C:N 25:1.
View Article and Find Full Text PDFZhongguo Yi Xue Ke Xue Yuan Xue Bao
October 2015
Objective: To determine the regulatory role and mechanism of nitric oxide (NO) in the development and hatching of mouse blastocysts.
Methods: The Kunming female mice were superovulated and then mated with mature male mice. On the day 2.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
August 2014
Background & Objective: Meisoindigo is a powerful drug used in treating chronic myeloid leukemia (CML), but little is known about the mechanisms. This study was to investigate the inducement effect of meisoindigo on apoptosis of myelocytic leukemia cell line HL-60, and explore the possible mechanisms.
Methods: After treatment of meisoindigo, the proliferation of HL-60 cells was detected by trypan blue exclusion assay, and DNA fragmentation by agarose electrophoresis; cell morphology was observed under fluorescent microscope.
The Epstein-Barr virus (EBV) BamHI A rightward transcripts (BARTs) were originally identified in C15 xenograft of nasopharyngeal carcinoma (NPC) and easily detected in a wide variety of EBV latent infection and EBV-associated tumors. It had been reported that p31 cosmid containing BARTs immortalized monkey epithelial cells, but which particular gene among BARTs family participates in the transformation procedure remains to be identified. RPMS1 is the only full-length cDNA confirmed so far and one of the most abundant spliced forms in BARTs family.
View Article and Find Full Text PDFEpstein-Barr virus (EBV) has been suggested to be involved in pathogenesis of nasopharyngeal carcinoma (NPC). However, EBV infection is ubiquitous, whereas NPC occurs with strong geographic and racial distribution. Whether a substrain of EBV contributes to this phenomenon remains uncertain.
View Article and Find Full Text PDFBackground & Objective: Epstein-Barr virus (EBV) A73 gene encodes for a main mRNA of BamHI A rightward transcripts (BARTs) family which is widely transcribed in EBV-carrying cells and tumors. This study was designed to investigate the expression of A73 mRNA in nasopharyngeal carcinoma(NPC) from Guangdong region,from which A73 coding sequence(CDS) was cloned and introduced into epithelial cell line to observe the subcellular fraction of its encoding protein.
Methods: DNA and total RNA were isolated from NPC and noncarcinoma nasopharyngeal tissues,peripheral blood lymphocyte of NPC, SUNE1, B95.