Publications by authors named "Hong-Zi DU"
Background: Cell-free nuclear DNA has been isolated from spent embryo culture medium. Whether this small amount of DNA can be amplified at the whole genome level and the concordance rate of karyotypes and specific alleles between biopsied cells and media has not been evaluated.
Methods: Seven couples were recruited, 88 donated embryos and their corresponding media were collected for whole genome amplification (WGA).
Article Synopsis
- Epigenetic regulation is essential for maintaining human pluripotent stem cells, with embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) showing hypermethylated states compared to differentiated cells.
- The study analyzed DNA methylation profiles of various human cell lines, finding that iPSCs share a similar hypermethylation status with ESCs but have distinct differences linked to their parental cells and the reprogramming process.
- This research offers insights into specific DNA methylation signatures that could inform the selection of optimal pluripotent stem cells for regenerative medicine.
[Effects of patient age, number and quality of embryo transferred on the outcome of in vitro fertilization-embryo transfer].
Nan Fang Yi Ke Da Xue Xue Bao
August 2010
Objective: To study the effect of patient age, the number and quality of embryo transferred on pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ETs).
Methods: A retrospective study was performed in infertile women who underwent a total of 1800 cycles of IVF-ET and intracytoplasmic sperm injection (ICSI). The patients were divided into three age groups, namely<30 years group, 30-35 years group, and>or=35 years group.
[Development and genetic polymorphism of abnormal pronuclear zygotes after intracytoplasmic sperm injection].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
August 2010
Objective: To compare the development of abnormal pronuclear zygotes after intracytoplasmic sperm injection (ICSI) and analyze their genetic polymorphism.
Methods: Four hundred and ninety three abnormal pronuclear zygotes after ICSI were divided into three groups based on the number of pronuclei: 347 nonpronuclear oocytes, 71 monopronuclear zygotes and 75 multipronuclear zygotes. All of them were cultured in the medium of Vitrolife G5 series(TM).
[In vitro maturation of human oocytes from pregnant late term: a new source of oocytes].
Nan Fang Yi Ke Da Xue Xue Bao
July 2009
Objective: To investigate the in vitro maturation of human oocytes (IVM) from pregnant late term, natural cycles and Gn stimulating cycles and the effect of granulose cells on IVM from pregnant late term.
Methods: A total of 1086 immature oocytes were obtained including 633 oocyte-cumulus complexes (OCCs) and 453 denuded oocytes (DOs). OCCs were divided into pregnant late term group, natural cycle group and IVM group, and DOs were divided into pregnant late term group, natural cycle group and controlled ovarian hyperstimulation (COH) group.
Objective: To investigate the effect of sperm acrosin activity on the IVF-ET outcome.
Methods: We analyzed sperm parameters, morphology and acrosin activity for 909 infertile husbands by computer-assisted self-assessment (CASA), modified Papanicolaou staining and N-alpha-benzoyl-DL-arginine-p-nitroanilide (BAPNA), respectively, and detected the rates of fertilization, cleavage, quality embryos, embryo cryopreservation, implantation, clinical pregnancy and abortion. The wives were identified as normal or with mere oviduct problems.