Multi-omics characterization of the unsaturated fatty acid biosynthesis pathway in colon cancer.

The biosynthesis of unsaturated fatty acids is involved in the initiation and progression of colon adenocarcinoma (COAD). In this study, we aimed to investigate the multi-omics characteristics of unsaturated fatty acid biosynthesis-related genes and explore their prognostic value in colon cancer by analyzing the data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. An unsaturated fatty acid biosynthesis pathway related-genes enrichment score (BUFAS) was constructed utilizing the single sample gene set enrichment analysis (ssGSEA).

Association of E1AF mRNA expression with tumor progression and matrilysin in human rectal cancer.

Objective: To examine E1AF mRNA expression and to determine whether it is correlated with tumor progression and matrilysin in human rectal cancer.

Methods: Real-time RT-PCR was used to determine E1AF and matrilysin expression in 100 matched rectal cancers and normal tissues.

Results: Among the 100 rectal cancers, 69 cases of E1AF mRNA overexpression were observed.

[Expression and clinical significance of matrilysin (MMP-7) in human rectal cancer].

Objective: To detect the matrilysin (MMP-7) expression in human rectal cancer and to investigate whether it is correlated with invasion and metastasis of human rectal cancer.

Methods: The paired samples obtained from 100 inpatients were allocated into cancer group and control group. By Quantitative-Real-Time RT-PCR, immunohistochemical staining and computerized image analysis, the mRNA and protein expressions of MMP-7 in human rectal cancer were measured and further analyzed for the relationship between MMP-7 expression and clinicopathologic characters.

Overexpression of connective tissue growth factor WISP-1 in Chinese primary rectal cancer patients.

Aim: To clarify the expression change of Wnt-induced secreted protein-1 (WISP-1) in human rectal cancer and to determine whether it is correlated with invasion and metastasis of human rectal cancer.

Methods: Eighty-six paired samples of rectal cancer and surgically resected distant normal rectal tissue were collected and allocated into cancer group and control group respectively. WISP-1 mRNA was detected by relative quantitative real-time RT-PCR and WISP-1 protein was examined by immunohistochemical staining.

Hepatic venous outflow reconstruction in right lobe graft without middle hepatic vein.

Background: We explored the pattern of hepatic venous outflow reconstruction in adult right lobe (segments V5-8) living donor liver transplantation (LDLT) without the middle hepatic vein (MHV). The difficulty and challenge of LDLT without MHV is the outflow reconstruction of hepatic vein. We have modified the surgical procedure and here report the results.

Clinicopathological characteristics of 15 patients with combined hepatocellular carcinoma and cholangiocarcinoma.

Background: Combined hepatocellular carcinoma and cholangiocarcinoma (cHCC-CC) is a rare subtype of primary liver cancer, and clinicopathological features of cHCC-CC have seldom been reported in detail. This study was undertaken to explore the diagnosis and clinicopathological characteristics of cHCC-CC in comparison with hepatocellular carcinoma (HCC) and cholangiocarcinoma (CC), respectively.

Methods: The clinical data from 15 patients with cHCC-CC, 132 patients with HCC and 44 patients with CC who had undergone hepatic resection were analyzed retrospectively.

[Quantification of PPARdelta mRNA by real-time RT-PCR in rectal cancer tissues].

Objective: To clarify the expression change of PPARdelta gene in human rectal cancer tissues and determine the correlation of PPARdelta expression with the clinical and pathological parameters of rectal cancer.

Methods: Applying real-time RT-PCR, we quantified PPARdelta mRNA in 86 tissues from excised primary rectal cancers. In each case, accompanying normal mucosa was collected for comparison.

Improved early diagnosis of cystadenocarcinoma of the pancreas.

Background: Cystadenocarcinoma of the pancreas is insensitive to radiotherapy and chemotherapy, and surgery is at present the definitive treatment. Early and accurate diagnosis of cystadenocarcinoma is crucial for increasing the five-year survival rate and the resectable rate. There is no definitive and effective method of early diagnosis of cystadenocarcinoma of the pancreas in China and other countries.

Caudate lobectomy by the third porta hepatis anatomical method: a study of 16 cases.

Background: The treatment for primary tumor in the caudate lobe of the liver is difficult because of its unique anatomical location. This study was undertaken to improve operative techniques and results by a new anatomical method of caudate lobectomy.

Methods: Clinical data of 16 patients who had had caudate lobectomy for the liver from January 1996 to November 2004 were retrospectively analyzed.

Clinicopathologic and prognostic significance of MMP-7 (matrilysin) expression in human rectal cancer.

Objective: There is increasing evidence to indicate that MMP-7 plays a more important role in tumor progression than other MMPs. The aim of this study was to detect MMP-7 expression in human rectal cancer and normal rectal tissue and to determine whether it is correlated with invasion and metastasis of human rectal cancer.

Methods: Eighty-six paired samples of rectal cancer and distant normal rectal tissue obtained from 100 inpatients were allocated into two groups (cancer group and control group).

Expression of HOXA11 gene in human endometrium.

Objective: This study was conducted to examine HOXA11 gene expression in the human endometrium during normal menstrual cycle.

Study Design: Expression of HOXA11 was examined in the endometrium by in situ hybridization and semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR).

Results: In the proliferative and early secretory endometrium, both glandular and stromal cells expressed HOXA11 after hybridization.