Publications by authors named "Hong-Yuan Hu"
Article Synopsis
- ZNF24 is a zinc finger transcription factor crucial for cell growth and differentiation, with its method of nuclear entry still not fully understood.
- Researchers identified that the nuclear localization signal (NLS) is located within the first two zinc finger domains (ZF1-2) of ZNF24, which are necessary for its movement into the nucleus.
- Mutations affecting the structure of ZF1 or ZF2 don't prevent nuclear localization, but certain mutations do lead to cytoplasmic accumulation, indicating that ZF1-2 is essential for ZNF24's nuclear function and its ability to activate gene expression.
[Cloning and superexpression of cry1Ac gene from 20kb DNA associated with Bacillus thuringiensis Cry1A Crystal Protein].
Sheng Wu Gong Cheng Xue Bao
September 2004
Article Synopsis
- The CrylA crystal protein from Bacillus thuringiensis is linked to unknown DNA sequences, which were studied by extracting and analyzing associated DNA from bipyramidal crystals.
- Using specific enzymes and cloning techniques, researchers created recombinant plasmids containing amplified cry1Ac gene fragments and produced high amounts of a fusion protein, which was confirmed to be a significant part of the cellular protein composition.
- Bioassays demonstrated that the inclusion bodies and crystals from the modified strains exhibited high toxicity against the larvae of Plutella xylostella, paving the way for further research into insecticidal applications.