[The Effect of KRAS on Proliferation and Apoptosis of T-ALL Cell Lines].

Objective: To investigate the function of RAS protein on the progression of the T-ALL cell lines in vitro.

Methods: The DNA of the T-ALL cells was purified then amplified the coding regions of three RAS genes (KRAS, NRAS, HRAS) by PCR reaction. After T-A cloning, the coding regions of KRAS, NRAS and HRAS were sequenced by Sanger Sequencing.

[Application of Flow Cytometry Combined Fluorescence in Situ Hybridization to Indentify the Lymphocyte Subtypies with Epstein-Barr Virus Infection].

Objective: To establish the technique that take the advantages of flow cytometry combined fluorescence in situ hybridization (Flow-FISH) to identify the Epstein-Barr virus(EBV) infected lymphocyte subtypies in patients' peripheral blood sample.

Methods: Peripheral Blood monocyte from 9 patients with EBV infection enrolled at Children's Hospital in Chongqing Medical University were isolated by Ficoll-paque centrifugal separation. The expressions of EBER1, EBER2 in cell were detected by qRT-PCR.

In vitro Antibiotic Susceptibility, Virulence Genes Distribution and Biofilm Production of Isolates from Bovine Mastitis in the Liaoning Province of China.

Purpose: The aim of this study was to identify the subtype, characterize the antimicrobial resistance, determine the virulence gene distribution, and analyze the biofilm production of isolates from bovine mastitis milk samples in the Liaoning Province of China.

Materials And Methods: In total, 56 isolates were collected and identified in this study; the isolates were divided into different types based on the sequence of the polymorphic X region of the gene. Additionally, antimicrobial susceptibility was investigated using the broth microdilution method, and 18 virulence genes were detected using PCR.