Nucleotide polymorphism-based study utilizes human plasma liposomes to discover potential therapeutic targets for intervertebral disc disease.

Background: While intervertebral disc degeneration (IVDD) is crucial in numerous spinally related illnesses and is common among the elderly, the complete understanding of its pathogenic mechanisms is still an area of ongoing study. In recent years, it has revealed that liposomes are crucial in the initiation and progression of IVDD. However, their intrinsic mediators and related mechanisms remain unclear.

Microstructure Evolution, Hot Deformation Behavior and Processing Maps of an FeCrAl Alloy.

The deteriorated plasticity arising from the insoluble precipitates may lead to cracks during the rolling of FeCrAl alloys. The microstructure evolution and hot deformation behavior of an FeCrAl alloy were investigated in the temperature range of 750-1200 °C and strain rate range of 0.01-10 s.

Ame-miR-980-3p participates in autophagy-mediated midgut remodelling in Apis mellifera via targeting Atg2B.

Autophagy is a process that serves to degrade damaged proteins and organelles, thereby promoting cell homeostasis, differentiation, development and survival. Many miRNAs have been found to have regulatory roles in autophagy. In insects, it has been shown that autophagy is involved in hormone-regulated programmed cell death during metamorphic midgut remodelling.

Retinoic Acid Receptor Gamma (RAR) Promotes Cartilage Destruction through Positive Feedback Activation of NF-B Pathway in Human Osteoarthritis.

Osteoarthritis (OA) is a severe inflammation-related disease which leads to cartilage destruction. The retinoic acid receptor gamma (RAR) has been indicated to be involved in many inflammation processes. However, the role and mechanism of RAR in cartilage destruction caused by inflammation in OA are still unknown.

[Investigation of Acaroid mites breeding in university canteen condiments].

Objective: To investigate the condition of Acaroid mite breeding in university canteen common condiments.

Methods: Thirteen kinds of condiments of the public cafeteria and 29 kinds of condiments of the canteen warehouse storage were collected. The mites were separated by the method of water film microscopy and an electric powder set, and then the film production; mite identification and counting were performed under a microscope.

[Investigation of natural foci of Metorchis taiwanensis in Wuhu area].

Objective: To explore the existence of natural foci of Metorchis taiwanensis in Wuhu area.

Methods: The ecological environment and daily living habits of residents were investigated; the eggs of M. taiwanensis in the dung of ducks in local region were collected by the sedimentation method; the Parafossarulus striatulus were disposed by the tabletting method and examined with a microscope to isolate the rediae and cercariae; the metacercariae in Pseudorasbora parve were isolated by using the homogenation.

[Immunotherapy effect of ploypeptide vaccine of allergen group 1 from Dermatophagoides farinae on asthma mice].

Objective: To investigate the enzymatic hydrolysates of ProDer f land their hydrolytic products for specific immunotherapy.

Methods: The asthma models of mice made by ProDer f 1 allergen were treated by using two kinds of hydrolysates as vaccine for analyzing their effects of immunotherapy. Fifty female BALB/c mice were randomly divided into 5 groups (n = 10 for each), i.

[Morphology observation of Histiostoma feroniarum hypopus].

Objective: To observe the external morphology of Histiostoma feroniarum hypopus under light microscope.

Methods: The samples were collected in a mushroom cultivation base, and the Histiostoma feroniarum hypopus was isolated and purified. The slide samples were prepared and observed under an optical microscope.

[Cloning and Prokaryotic Expression of Fusion Gene of Group II Allergen Der p2 T Cell Epitope from Dermatophagoides pteronyssinus].

Objective: To express and purify the T cell epitope fusion peptide of the major allergen Der p2 from Dermatophagoides pteronyssinus.

Methods: Nucleotide sequences reported to encode four T-cell epitopes (T1-T4) of Der p2 of D. pteronyssinus were linked in the rank of T1-T2-T3-T4.

[Detection of DNA-protein crosslinks with modified comet assay].

Modifications of the comet assay were introduced to measure crosslinks and the effect of formaldehyde in liver cells of the tested animals was investigated by the new method to see whether the method is feasible. Since exposure of slides to proteinase K can increase DNA migration of the treated cells, the presence of DNA-protein crosslinks can be indicated by compare of the tail moment before and after the proteinase K added. The results showed that the modified protocol of the alkaline comet assay is a fast, inexpensive and sensitive tool for the detection of potent crosslinkers-induced DNA-protein crosslinks at single cell level.

Analysis of the Exo(-) Mutant of Rhizobium huakuii and Their Exopolysaccharide Component.

We have subcloned two plasmids, pWS-6BP1 and pWS-6BP2, from the 8.5 kb DNA fragment of plasmid pJB22-B6. The plasmid of pWS-6BP1, with a 5.

Cloning and Sequencing of Rhizobium huakuii exoA Gene Encoding a Glucosyl-transferase.

From exoR'-11 which could complement two Exo(-) mutants of R. huakuii 107: NA03 and NA10, a 2.0kb BglI fragment was subcloned in pRK415.

Comparison of Different Effects of Chloroacetates on Electron Transports in PS II and in the Reaction Center of Rb. sphaeroides 601.

Chloroacetates displayed different effects on electron transports in the photosynthetic reaction center of purple bacteria (Rb.sphaeroides 601) and in the photosystem II (PS II) of higher plants. Decays of chlorophyII a fluorescence measured after actinic flashes show that chloroacetates inhibit the electron transport from Q(A)(-) to Q(B) (Q(B)(-)) and the equilibrium between Q(A)(-)Q(B) (Q(B)(-)) and Q(A)Q(B)(-) (Q(B)(2-)), acting on electron transport as well as proton transduction.

Sequence Analysis of the exo1 Gene Involved in the Exopolysaccharide Synthesis of Rhizobium huakuii.

The 2.6 kb fragment which can complement the Exo(-)Ndv(-)Fix(-) mutants of R. huakuii to Exo(+)Nod(+)Fix(+) was sequenced.