Efficacy and safety of erector spinae plane block for postoperative analgesia in breast cancer surgery-A systematic review and meta-analysis.

Background And Objectives: We aim to evaluate the analgesic efficacy and safety of erector spinae plane block (ESPB) for postoperative analgesia in breast cancer surgeries.

Methods: PubMed, Web of Science, CBM, Embase, Cochrane, Wanfang, VIPP, and CNKI were searched to identify published eligible randomized controlled trials. The primary results were the postoperative 24 h morphine consumption and pain scores, while the secondary outcomes included pain scores at other times, press times of patient-controlled intravenous analgesia (PCIA), times to request for first rescue analgesia, the incidence of request for rescue analgesia, opioid-related complications, nerve blocks related complications and patient satisfaction.

Facile cyclization of sodium aminodiboranate to construct a boron-nitrogen-hydrogen ring.

A facile and efficient cyclization of sodium aminodiboranate to construct a boron-nitrogen-hydrogen ring is presented. This new strategy can be developed into a general method to prepare aminodiborane and its derivatives. Theoretical calculations show that a one-step cyclization mechanism is favored, where the dihydrogen bond plays an important role.

Eudesmane-type sesquiterpene diols directly synthesized by a sesquiterpene cyclase in .

Cryptomeridiol, a typical eudesmane diol, is the active principle component of the antispasmodic Proximol. Although it has been used for many years, the biosynthesis pathway of cryptomeridiol has remained blur. Among terpenoid natural products, terpenoid cyclases are responsible for cyclization and generation of hydrocarbon backbones.

[Cloning and protein expression analysis of monoterpene synthase gene TwMS in Tripterygium wilfordii].

In this study, we cloned a monoterpene synthases, TwMS from Tripterygium wilfordii suspension cells. TwMS gene contained a 1 797 bp open reading frame (ORF), encoding a polypeptide of 579 amino acids, which deduced isoelectric point (pI) was 6.10 and the calculated molecular weight was 69.

[Cloning and protein expression analysis of geranyl diphosphate synthase genes in Tripterygium wilfordii].

Based on the transcriptome data, the study cloned full-length cDNA of TwGPPS1 and TwGPPS2 genes from Tripterygium wilfordii suspension cells and then analyzed the bioinformation of the sequence and protein expression. The cloned TwGPPS1 has a 1 278 bp open reading frame (ORF) encoding a polypeptide of 425 amino acids. The deduced isoelectric point (pI) was 6.

[Cloning and expression analysis of kaurenoic acid oxidase gene in Tripterygium wilfordii].

Kaurenoic acid oxidase involved in biosynthesis pathway of gibberellin. According to the transcriptome database, the specific primers were designed and used in cloning the full-length cDNA of TwKAO, the bioinformatic analysis of the sequence was performed. The qRT-PCR were used to detect the expression level of TwKAO after MeJA treatment.

Cloning and functional analysis of two sterol-C24-methyltransferase 1 (SMT1) genes from Paris polyphylla.

The biosynthetic pathways of phytosterols and steroidal saponins are located in two adjacent branches which share cycloartenol as substrate. The rate-limiting enzyme S-adenosyl-L-methionine-sterol-C24-methyltransferase 1 (SMT1) facilitates the metabolic flux toward phytosterols. It catalyzes the methylation of the cycloartenol in the side chain of the C24-alkyl group, to generate 24(28)-methylene cycloartenol.

[Cloning and protein expression of sterol-C-24-methyl transferase 2 in Tripterygium wilfordii].

24-Alkyl sterols are the major players in the control of membrane component and plant growth. In this paper, we cloned an important rate-limiting enzyme: sterol-C-24-methyl transferase (SMT) in the sterol biosynthetic pathway according to the transcriptome data of Tripterygium wilfordii. suspension cells, whose full-length c DNA was 1 631 bp with an open reading frame of 1 080 bp, encoding a protein of 359 amino acids.

[Cloning and protein expression of sterol-C-24-methyl transferase 2 in Tripterygium wilfordii].

24-Alkyl sterols are the major players in the control of membrane component and plant growth. In this paper, we cloned an important rate-limiting enzyme: sterol-C-24-methyl transferase (SMT) in the sterol biosynthetic pathway according to the transcriptome data of Tripterygium wilfordii. suspension cells, whose full-length cDNA was 1 631 bp with an open reading frame of 1 080 bp, encoding a protein of 359 amino acids.

Synthesis of chelating complexes through solid-state dehydrochlorination reactions via second-sphere-coordination interaction with metal chlorides: a combined experimental-molecular modeling study.

We have applied crystal engineering as a tool to study the solid-state transformation from molecular salts to coordination complexes via mechanochemical dehydrochlorination reactions. The -(CH2)n- (n = 2, 3) alkyl chains were introduced into the bibenzylamine moiety to form the two nitrogen bases N,N,N',N'-tetrabenzylethylenediamine (L(1)) and N,N,N',N'-tetrabenzylpropydiamine (L(2)), which were self-assembled with tetrachlorometalates to form a series of supramolecular salts through second-sphere coordination. Single crystals of salts [L(1)]2H(+)·[CuCl4](2-)·solvent (1) and [L(2)]2H(+)·[XCl4](2-)·solvent (2-4; X = Cu, Hg, Zn) were obtained and their structures determined by single-crystal X-ray diffraction.

[The study of the characteristics and influencing factors of pneumoconiosis among workers exposed to dusts in an iron mine].

Objective: To analyze the prevalence characteristics and influence factors of pneumoconiosis of workers exposed to dusts in an iron mine, to provide the base of preventive measures for pneumoconiosis of iron mine.

Methods: The subjects of cohort study were all workers exposed to dusts for at least one year registered in an iron mine during 1960 to 1974, and followed-up to the end in 2003. The cases with pneumoconiosis were diagnosed by the local diagnosis group of pneumoconiosis, according to the national diagnostic criteria of pneumoconiosis.

A LDR-PCR approach for multiplex polymorphisms genotyping of severely degraded DNA with fragment sizes <100 bp.

Reducing amplicon sizes has become a major strategy for analyzing degraded DNA typical of forensic samples. However, amplicon sizes in current mini-short tandem repeat-polymerase chain reaction (PCR) and mini-sequencing assays are still not suitable for analysis of severely degraded DNA. In this study, we present a multiplex typing method that couples ligase detection reaction with PCR that can be used to identify single nucleotide polymorphisms and small-scale insertion/deletions in a sample of severely fragmented DNA.

[Polymorphism of TPH gene T3792A locus in Han ethnic group of north China].

Objective: To investigate the polymorphism of the TPH gene T3792A locus in Han ethnic group of north China and its application value in forensic science.

Methods: The polymorphism of T3792A locus of the TPH gene was analyzed by the ASPCR of blood samples from 173 unrelated individuals of north Chinese Han population.

Results: The distribution of the T3792A locus polymorphism of the TPH gene in Han ethnic group of north China followed the Hardy-Weinberg law, with the allele A and T gene frequency of 0.

Clinical significance of sphingosine kinase-1 expression in human astrocytomas progression and overall patient survival.

Purpose: To characterize the expression of sphingosine kinase-1 (SPHK1) in human astrocytomas and to investigate the association between SPHK1 expression and progression of astrocytomas.

Experimental Design: The expression of SPHK1 in normal human astrocytes, astrocytoma cell lines, and four pairs of matched astrocytoma tissues and their adjacent normal brain tissues were detected by quantitative reverse transcription-PCR and Western blot. In addition, SPHK1 protein expression was examined in 243 cases of histologically characterized astrocytomas by immunohistochemistry.

[Distribution of tryptophan hydroxylase gene-T457C locus polymorphism in Han ethnic group in northern China].

Objective: To study the distribution of the tryptophan hydroxylase (TPH) gene-T457C locus polymorphism in Han ethnic group in northern China and to find its applicable value in forensic science.

Methods: Genomic DNA samples, extracted from 180 unrelated individuals in northern Chinese Han population, were analyzed by PCR-RFLP.

Results: The discrimination power (DP) value and the power of exclusion (PE) value of the TPH gene-T457C locus were 0.

[Cohort mortality study of dust exposed miners in iron mine].

Objective: To investigate the main diseases jeopardizing the health of the iron miners and to explore the relationship between dust exposure and malignancies as well as other diseases.

Methods: A retrospective study with a cohort of 7,469 workers employed between January 1, 1972 and December 31, 1974 in Daye Iron Ore Mine Co. in Hubei Province was conducted.