Three new species of the genus Oedichirus Erichson, 1839 (Coleoptera: Staphylinidae: Paederinae: Pinophilini) from China.

Three new species of Oedichirus Erichson, 1839 are described: Oedichirus haishengensis Duan, Yue & Li sp. nov. from Guangxi Province, O.

Oxytelus sishuishanus, a new species of Oxytelus Gravenhorst, 1802 (Coleoptera: Staphylinidae: Oxytelinae) from Sishui Mountain, China.

A new species, Oxytelus sishuishanus Lü, is described from Hunan, China. This species closely resembles O. ailaoshanicus Lü & Zhou, 2012, but can be distinguished by the male clypeus, which is not so protruding as in O.

Transcriptomic and Metabolomic Analyses Provide Insights into the Formation of the Peach-like Aroma of Schlecht. Fruits.

Schlecht. is a wild diploid strawberry species. The intense peach-like aroma of its fruits makes an excellent resource for strawberry breeding programs aimed at enhancing flavors.

Key residues responsible for enhancement of catalytic efficiency of Thermomyces lanuginosus lipase Lip revealed by complementary protein engineering strategy.

The variant Lip-T (S88T/A99N/V116D) of lipase Lip from Thermomyces lanuginosus has been proved to be a potential biocatalyst for kinetic resolution of 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester (CNDE) to produce valuable chiral intermediate of Pregabalin. In this study, random, site-directed and site-saturation mutagenesis were performed to further enhance the activity of Lip-T, and the key residues responsible for catalytic efficiency were revealed. A mutant S63L/D232A with improved activity toward CNDE was obtained after screening of approximately 2500 clones from random-mutant libraries.

Engineering of Thermomyces lanuginosus lipase Lip: creation of novel biocatalyst for efficient biosynthesis of chiral intermediate of Pregabalin.

Efficient and highly enantioselective hydrolysis of 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester (CNDE) is the most crucial step in chemoenzymatic synthesis of Pregabalin. By using site-saturation mutagenesis and high-throughput screening techniques, lipase Lip from Thermomyces lanuginosus DSM 10635 was engineered to improve its activity towards CNDE. The triple mutant, S88T/A99N/V116D exhibited a 60-fold improvement in specific activity for CNDE (2.