[Preparation of antibody against Memo protein and analysis of expression profile of Memo in mouse tissues].

Aim: To prepare and characterize antibody against Memo protein and to detect the tissue distribution of Memo in mice.

Methods: Fusion protein GST-Memo was expressed and purified, and polyclonal antibody against Memo was prepared by immunizing mice. A FLAG-tagged eukaryotic expression vector pcDNA3-FLAG-Memo was constructed.

Differentiation of mesenchymal stem cells into dopaminergic neuron-like cells in vitro.

Objective: To explore the way to induce mesenchymal stem cells (MSCs) to differentiate into dopaminergic neurons in vitro.

Methods: MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experiment had multipotency, which was indirectly proved by being induced to differentiate into chondrocytes and adipocytes.

Induction of tolerance in quadruple chimeric mice.

Human cord blood (CB) contains hematopoietic stem cells and progenitors. Because the major limitation to a widespread use of CB for transplantation lies in its limited volume, it is necessary to combine the CB from several donors. In this study, we show that lethally irradiated mice can be reconstituted with the injection of a mixture of T cell-depleted bone marrow cells (BMCs; total, 3 x 10(6)) obtained from three fully allogeneic mouse strains in two different mouse combinations.