Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
June 2004
Background: To construct the full-length complementary DNA of HCV genome from an HCV infected patient.
Methods: Four HCV gene fragments (1.6, 3.
Aim: To establish an efficient, sensitive, cell-based assay system for NS3 serine protease in an effort to study further the property of hepatitis C virus (HCV) and develop new antiviral agents.
Methods: We constructed pCI-neo-NS3/4A-SEAP chimeric plasmid, in which the secreted alkaline phosphatase (SEAP) was fused in-frame to the downstream of NS4A/4B cleavage site. The protease activity of NS3 was reflected by the activity of SEAP in the culture media of transient or stable expression cells.