[Aesthetic effect of wound repair with flaps].

Objective: To investigate the aesthetic effect of wound repair with flaps.

Methods: One thousand nine hundred and ninety-six patients with 2082 wounds hospitalized from January 2004 to December 2011. These wounds included 503 deep burn wounds, 268 pressure sores, 392 soft tissue defects caused by trauma, 479 soft tissue defects due to resection of skin cancer and mole removal, 314 soft tissue defects caused by scar excision, and 126 other wounds.

[Effect of platelet-derived growth factor-BB on proliferation of tendon cells cultured in vitro].

Objective: To study the effect of platelet-derived growth factor-BB (PDGF-BB) in different concentrations on proliferation of tendon cells cultured in vitro.

Methods: Rat tendon cells were cultured and identified in vitro. The rat tendon cells were cultured in PDGF-BB nutrient solution in different concentrations.

[Clinical application of Meek micrograft technique].

Objective: To explore the clinical application of the transplantation of the Meek autograft.

Methods: Meek autografts were transplanted on the full-thickness wounds of 10 patients, including 9 cases of burn and 1 case of necrofascitis, with the area of 8.6% (2% - 28%) of the total body surface, and the average of the area was.

[Effect of expression of platelet-derived growth factor B gene on blood vessel reconstruction after tissue engineering skin grafting].

Objective: To study the effect of PDGF on dermal blood vessel reconstruction by transplanted tissue-engineering skin containing PDGF-B gene to rats.

Methods: The recombined eukaryotic expression vector, pcDNA3.1-hPDGF-B, was constructed and transfected into fibroblasts mediated by LipofectAMINE.

[Preparation of porcine acellular dermal matrix by low concentration of trypsin digestion and repeated freeze-thaw cycles].

Objective: To establish a new method for the preparation of porcine acellular dermal matrix.

Methods: The antigenicity of the porcine dermis was weakened by removing epidermal and dermal cells from the porcine skin through the digestion with low-concentration trypsin and repeated freeze-thaw cycles. Split thickness porcine skin was treated with 0.

[An experimental study of cultured keratinocytes on the acellular xenodermis].

Objective: To culture the keratinocytes on the acellular pig dermis and establish a composite skin in vitro.

Methods: Full thickness skin taken from neonatal SD rats of approximate 24-hour-old was cultivated in asepsis condition, which was then separated into epidermal and dermal layers with low-temperature enzyme digestion. The basal lamina cells between the two layers were scraped off and the pure keratinocytes were obtained using gradient density centrifugation.