Cloning and Expression of the cDNA Coding for Rat Peptidylglycine alpha-Amidating Monooxygenase.

The dDNA of genes encoding rat peptidylglycine alpha-aminating monooxygenase(rPAM) were cloned and their expression in E. coli studied. Three DNA fragments were isolated from the rat brain cDNA library using the methods of plaque hybridization and PCR.