Harzianic acids and oxazolidinone from the endophytic fungus Ilyonectria sp. and their cytotoxicity activity.

Four undescribed compounds including three harzianic acids (1, 3 and 4) and one oxazolidinone (2), along with three known ones (5-7) were isolated from the solid fermented product of endophytic fungus Ilyonectria sp., their structures were elucidated as 1-amino-harzianic acid (1), ilyonectria-oxazolidinone (2),10'-nor- isoharzianic acid (3), isohomoharzianic acid (4), harzianic acid (5), isoharzianic acid (6), homoharzianic acid (7) by means of detailed chemical evidences and spectroscopic data analysis. All the compounds were evaluated for cytotoxicity against SMMC-7721 human cancer cell lines by MTS assay.

Chromones from the endophytic fungus Bipolaris eleusines.

Eight previously undescribed chromones eleusineketones A-H (1-8), as well as eight known compounds (9-16), were isolated from the endophytic fungus Bipolaris eleusines. These planar structures were created using an in-depth analysis of their spectral data, which included 1D, 2D, and HRESIMS data. Furthermore, the absolute configurations of compounds 1, 2, and 6 were determined by spectroscopic analysis and quantum chemical computational approaches, and compound 5 was determined by single-crystal X-ray diffraction analysis.

The antifungal properties of terpenoids from the endophytic fungus Bipolaris eleusines.

A series of terpenoids (1-17), comprising six new compounds designated bipolariterpenes A-F (1-6) and eleven recognized compounds (7-17), were isolated from the wheat culture of the potato endophytic fungus Bipolaris eleusines. Their structures and stereochemistry were clarified by HRESIMS, NMR, DP4 + probability analyses, and computations for electronic circular dichroism (ECD). All compounds are made up of six meroterpenoids, four sesterterpenes and seven sesquiterpenes.

Boerelasins A-D, Four Unprecedented Cytochalasins from the Endophytic Fungus .

Four undescribed cytochalasins () were isolated from the endophytic fungus . Structurally, boerelasin A () represents the first example of a cytochalasin with a rare 5/5 bicyclic carbon core. Boerelasin B () possesses an unprecedented 5/6/5/6/8 pentacyclic ring system.

Pimarane Diterpenes from Fungi.

Pimarane diterpenes are a kind of tricyclic diterpene, generally isolated from plant and fungi. In nature, fungi distribute widely and there are nearly two to three million species. They provide many secondary metabolites, including pimarane diterpenes, with novel skeletons and bioactivities.

Paecilins F-P, new dimeric chromanones isolated from the endophytic fungus E10, and structural revision of paecilin A.

A total of eleven new dimeric chromanones, paecilins F-P (-), were isolated from the endophytic fungus E10, along with four known analogs (, -). Their structures and absolute configurations were determined by extensive experimental spectroscopic methods, single-crystal X-ray diffraction, and equivalent circulating density (ECD) calculations. In addition, the structure of paecilin A, which was reported to be a symmetric C8-C8' dimeric pattern, was revised by analysis of the nuclear magnetic resonance (NMR) data, and single-crystal X-ray diffraction.

Four New Highly Oxygenated Eremophilane Sesquiterpenes from an Endophytic Fungus Isolated from .

Four new eremophilane-type sesquiterpenes, boeremialanes A-D (-) were obtained from solid substrate cultures of (Didymellaceae), an endophytic fungus isolated from (Liliaceae). Boeremialanes A-C (-) are highly oxygenated eremophilanes with a benzoate unit attached at the C-13 position and are rarely found in nature. Their structures and absolute configurations were determined by extensive spectroscopic methods, electronic circular dichroism (ECD), and nuclear magnetic resonance (NMR) calculations with DP4+ analysis.

Three new pyrrole alkaloids from the endophytic fungus Albifimbria viridis.

Three new pyrrole alkaloids albifipyrrols A-C (1-3), were isolated from the endophytic fungus Albifimbria viridis collected from the Chinese medicinal plant. Their structures were elucidated by extensive NMR and HRESIMS spectrometric analyses. All compounds were evaluated for immunosuppressive activity.

Oblongolides from endophytic fungus Phoma bellidis Neerg. harbored in Tricyrtis maculata (D. Don) J.F.Macbr.

Five previously undescribed oblongolides, namely phomaones A-E, along with four known compounds, were isolated from the endophytic fungus Phoma bellidis Neerg.. Their structures and absolute configurations were determined by extensive experimental spectroscopic methods as well as single crystal X-ray diffractions, ECD calculations and GIAO C NMR calculations.

Immunosuppressive Isopimarane Diterpenes From Cultures of the Endophytic Fungus .

Five new isopimarane diterpenes, robustaditerpene A-E (-), which include 19-nor-isopimarane skeleton and isopimarane skeleton, were isolated from the liquid fermentation of the endophytic fungus collected from The structure elucidation and relative configuration assignments of all compounds were accomplished by interpretation of NMR and HRESIMS spectrometric analyses and C NMR calculation. And the absolute configuration of - were identified by single-crystal X-ray diffraction and ECD calculation. Compound inhibited lipopolysaccharide-induced B lymphocytes cell proliferation with an IC value at 17.

Ophiorrhines F and G, Key Biogenetic Intermediates of Ophiorrhine Alkaloids from and Their Immunosuppressant Activities.

Continued interest in bioactive alkaloids led to the isolation of two undescribed alkaloids, ophiorrhines F () and G (), from the aerial parts of Their structures were elucidated based on spectroscopic methods, electronic circular dichroism, and calculated NMR with DP4+ analysis. These two alkaloids represent key biological genetic intermediates in the formation of ring C in the ophiorrhines. Compound exhibited good inhibition on LPS-induced B cell proliferation with an IC value of 0.

Structure and cytotoxicity of trichothecenes produced by the potato-associated fungus Trichothecium crotocinigenum.

Seven previously undescribed trichothecenes, named trichothecrotocins M-S (1-7), along with five known compounds, were isolated from rice cultures of the potato-associated fungus Trichothecium crotocinigenum. Their structures and absolute configurations were determined through spectroscopic methods, single-crystal X-ray diffraction, and quantum chemistry calculations on ECD. Compound 1 possesses a rare 6,11-epoxy moiety in the trichothecene family.

Identification and Bioactivities of Secondary Metabolites Derived from Endophytic Fungi Isolated from Ethnomedicinal Plants of Tujia in Hubei Province: A Review.

Tujia is a national minority, inhabiting in the mountainous Wuling area in China. Since 1978, Tujia medicine has been studied, summarized and developed, leading to numerous achievements by Chinese researchers, such as the publishing of approximately 30 monographs of Tujia medicine. These publications are focused on summarizing and improving the theory of Tujia medicine and developing clinical therapies from this system of medicine.

Characterization of the complete chloroplast genome of a well-known Chinese medicinal herb, .

has been used as a famous traditional medicinal herb in Asian countries to treat jaundice, dysentery, and the pain of rheumatism. In this paper, the complete chloroplast (cp) genome sequence of was reported and characterized. The cp genome is 153,175 bp in length, composed of a pair of 25,490 bp inverted repeat (IR) regions separated by a large single copy (LSC) region of 84,257 bp and a small single copy (SSC) region of 17,938 bp.

The complete chloroplast genome sequence of a traditional Chinese medicine: (Amaranthaceae).

(Amarathaceae) has been commonly used as a traditional Chinese medicine in the treatment of osteoporosis and bone nonunion. Here, the complete chloroplast genome of was assembled and characterized. The cp genome is 151,451 bp in length, composed of a pair of 25,150 bp inverted repeat (IR) regions separated by a large single-copy (LSC) region of 83,899 bp and a small single-copy (SSC) region of 17,252 bp.

The complete plastid genome of : a traditional Chinese medicine.

has been used as Chinese traditional medicine to treat inflammation and throat disorders for many centuries. In this study, the complete plastid genome of was first reported and characterized. The complete plastid genome is a typical quadripartite circular molecule of 153,729 bp in length, including a large single copy (LSC) region of 83,136 bp and a small single copy (SSC) region of 18,165 bp separated by two inverted repeat (IR) regions of 26,214 bp.

Trichothecrotocins D-L, Antifungal Agents from a Potato-Associated .

Seven new merosesquiterpenoids, trichothecrotocins D-J (-), two new trichothecene sesquiterpenoids, trichothecrotocins K () and L (), and six known compounds (-, , and ), were isolated from a potato-associated fungus, . Compounds and were racemates which were further separated as pure enantiomers. Structures together with absolute configurations were established by extensive spectroscopic analysis, as well as quantum chemistry calculations on ECD and optical rotations.

Depsidones and diaryl ethers from potato endophytic fungus Boeremia exigua.

Three depsidones boremexins A-C (1-3), two diaryl ethers boremexins D (4) and E (5), together with four known compounds were obtained from cultures of potato endophytic fungus Boeremia exigua. Their structures with absolute configurations were established by extensive spectroscopic methods and electronic circular dichroism (ECD) calculations. Compounds 1-4, 6, and 9 displayed anti-inflammatory properties on nitric oxide production in LPS-induced RAW264.

Novel Metabolites from the Endophytic Fungus Chaetomium subaffine L01.

One natural p-terphenyl glycoside, gliocladinin C, and two furano-polyene derivatives, chaetominins A and B, were isolated from potato endophytic fungus Chaetomium subaffine. The absolute configurations of these compounds were elucidated by HR-ESI-MS, NMR, the DP4+ probabilities and electronic circular dichroism (ECD) spectra. Furthermore, gliocladinin C and chaetominin A showed cytotoxic activity against two selected human tumor cell lines (Hep-2 and HepG-2).

Curtachalasins, immunosuppressive agents from the endophytic fungus Xylaria cf. curta.

Eleven new cytochalasins, curtachalasins F-P (1-11), were isolated from the rice fermentation of endophytic fungus Xylaria cf. curta. Their structures were identified by extensive spectroscopic methods, X-ray diffraction, and quantum chemistry calculations.

Xylarichalasin A, a Halogenated Hexacyclic Cytochalasan from the Fungus cf. .

A cytochalasan, xylarichalasin A, was obtained from the endophytic fungus cf. harbored in . Its structure was elucidated by comprehensive spectroscopic methods including HRESIMS, 1D/2D NMR, and residual dipolar coupling analysis as well as quantum chemistry calculations including DFT GIAO C NMR and ECD calculation.

Cytotoxic 19,20-epoxycytochalasans from endophytic fungus Xylaria cf. curta.

Nine new 19,20-epoxycytochalasans (1-9) were isolated from the rice fermentation extracts of endophytic fungus Xylaria cf. curta, along with four known compounds 19,20-epoxycytochalasin C (10), 18-desoxy-19,20-epoxycytochalasin C (11), 19,20-epoxycytochalasin D (12) and 5,6-dihydro-7-oxo-19,20-epoxycytochalasin C (13). Their structures and absolute configurations were determined by 1D and 2D NMR, HRESIMS, X-ray diffraction and ECD calculation.

Cytochalasans from the Endophytic Fungus Xylaria cf. curta with Resistance Reversal Activity against Fluconazole-Resistant Candida albicans.

Curtachalasins C-E (1-3), which have an unprecedent bridged 6/6/6/6 ring system, were identified from the endophytic fungus Xylaria cf. curta. Residual dipolar coupling analyses associating with density functional theory calculations were utilized to determine the relative configuration of noncrystallizable compound 2.

Anti-phytopathogenic sesquiterpenoid-xanthone adducts from potato endophytic fungus .

A bioguided separation on the cultures of the potato endophytic fungus led to the isolation of two anti-phytopathogenic () sesquiterpenoid-xanthone adducts, namely bipolins I and J (1 and 2). Their structures were established extensive spectroscopic analysis. Compounds 1 and 2 exhibit potent inhibitory activity against with MIC values of 8 and 16 μg mL, respectively.