Identification, Biological Characteristics, and Active Site Residues of 3-Ketosteroid Δ-Dehydrogenase Homologues from .

3-Ketosteroid Δ-dehydrogenase (KsdD) is the key enzyme responsible for Δ-dehydrogenation, which is one of the most valuable reactions for steroid catabolism. has been widely used in the industry due to its superior bioconversion efficiency, but KsdD information is not yet fully clear. Here, five KsdD homologues were identified in CGMCC 14539.

Compatible solutes adaptive alterations in Arthrobacter simplex during exposure to ethanol, and the effect of trehalose on the stress resistance and biotransformation performance.

Ethanol-tolerant Arthrobacter simplex is desirable since ethanol facilitates hydrophobic substrates dissolution on an industrial scale. Herein, alterations in compatible solutes were investigated under ethanol stress. The results showed that the amount of trehalose and glycerol increased while that of glutamate and proline decreased.