Publications by authors named "Hong-Hoa Hoang"
Article Synopsis
- Adipogenesis, the process of fat cell formation, involves significant changes in gene expression and cell structure, but current methods for analyzing live cell images are limited.
- Researchers used holotomography, a non-invasive imaging technique, to identify distinct markers of each stage in the transformation of stem cells from human umbilical cords into fat cells, revealing specific changes in cell shape and lipid droplet characteristics.
- The findings indicate clear morphological differences between preadipocytes, immature, and mature adipocytes, showing potential for advancing medical treatment strategies for metabolic diseases through better understanding of fat cell development.
Spheroids are recognized for replicating the physiological microenvironment of tumours. However, because of the lack of controllability of the spheroid size, the response to anticancer drugs is variable in conventional spheroid culture methods. In this paper, we describe a method to generate several hundreds of spheroids of various types of cancer cells including patient derived cancer cells (PDCs) using a microfluidic device with pillars (diameter: 40 μm, height: 70 μm, center-to-center distance: 140 μm), called a microfluidic pillar array (μFPA) device.
View Article and Find Full Text PDFIn vitro prediction of the probable rapid emergence of resistance to a drug in tumors could act to winnow out potential candidates for further costly development. We have developed a microfluidic device consisting of ∼500 hexagonal microcompartments that provides a complex ecology with wide ranges of drug and nutrient gradients and local populations. This ecology of a fragmented metapopulation induced the drug resistance in stage IV U87 glioblastoma cells to doxorubicin in 7 d.
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