[The degree of inbreeding in 10 Chinese indigenous pig breeds].

The population inbreeding coefficients of 10 indigenous pig breeds were estimated by microsatellite markers. Results showed that: the inbreeding coefficient of Guizhou miniature pig was the highest (0.1992), and that of Hanzhong black pig the lowest (0.

[Phylogenesis analysis of 10 chinese indigenous pig populations].

Genetic relationship of 10 indigenous pig populations was analyzed by 10 microsatellite markers. Results showed that different phylogenetic methods led to different results. The NJ phylogenetic tree from Nei's genetic distance was consistent with the facts.

[Sequence analysis of polymorphic fragments of porcine H-FABP gene].

Three variant restriction sites of porcine H-FABP gene,including HinfI-RFLP in 5'-upstream, HaeIII-RFLP and HinfI*-RFLP in intron 2, were confirmed by PCR-RFLP method. The polymorphic fragments were cloned and sequenced. The results revealed a single nucleotide substitution of T-->C at position 1324 for HinfI-RFLP,C-->G at position 1811 for HaeIII-RFLP and T-->C at position 1970 for HinfI*-RFLP, respectively.

[Genetic variation of A-FABP microsatellites in eleven pig breeds].

The genetic variations of microsatellites in intron 2 of the porcine adipocyte fatty acid-binding protein (A-FABP) genes were investigated in 420 pigs including Wuzhishan pig, Yimeng black pig, Hanjiang black pig, Laiwu pig, Beijing black pig, Min pig, Chenghua pig, Neijiang pig, Erhualian pig, Bama xiang pig and Large White pig. The results suggested as follows: (i)PIC of the Wuzhishan pig breed is the highest (0.7904) and 11 alleles were detected.