Publications by authors named "Hong-Da Wang"

Article Synopsis
  • Chronic rejection (CR) is a major challenge for allograft survival, and oxymatrine (OMT), a compound from traditional Chinese medicine, shows promise for treating this condition.
  • In experiments with B6 mice, OMT was found to reduce pathological damage in heart transplants, inhibit harmful T cell activities, and enhance regulatory T cell differentiation, leading to improved graft survival.
  • OMT appears to work by blocking specific signaling pathways (like mTOR and TGF-β-Smad 2/3), which helps decrease immune responses and fibrosis in the transplanted tissues, indicating potential for future therapeutic approaches.
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  • Exosomes derived from endometrial regenerative cells (ERC-Exos) have potential immunomodulatory effects, particularly in treating inflammatory bowel diseases like colitis, and the role of insulin-like growth factor-Ⅱ (IGF2) in this process was explored.
  • In experiments with mice experiencing colitis, treatment with ERC-Exos led to significant improvements in symptoms and intestinal health compared to untreated mice, but the protective effects were reduced when IGF2 was knocked out from the exosomes.
  • Additionally, the presence of IGF2 in ERC-Exos was found to influence immune cell populations, reducing harmful T helper cell types and dendritic cell activity, suggesting a beneficial immunological shift towards a more anti-inflammatory environment
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  • A new ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was developed for analyzing chemical components in Euodiae Fructus.
  • The study optimized various chromatographic and mass spectrometry conditions, ultimately using specific columns and mobile phases for effective separation and detection.
  • A total of 92 compounds, including 33 alkaloids and 23 flavonoids, were characterized, with 11 being newly identified, advancing understanding of Euodiae Fructus's chemical profile and supporting quality control efforts.
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  • Allograft rejection is a significant challenge in organ transplantation, and this study investigates the effects of recombinant human IL-37 (rhIL-37) in improving graft survival in a mouse model.
  • The research found that rhIL-37 treatment led to long-term acceptance of transplanted hearts by reducing immune cell infiltration and modulating cytokine profiles, specifically increasing Treg cells and decreasing inflammatory cytokines like TNF-α.
  • The findings suggest that IL-37 may be a promising therapeutic option for preventing organ rejection, highlighting its role in immune regulation through pathways like p-mTOR.
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  • Dragon's Blood (DB) is a valuable Chinese medicine known for improving blood circulation and reducing blood stasis, derived from two main plant sources: Daemonorops draco and Dracaena cochinchinensis.
  • The study utilized advanced techniques, including two-dimensional liquid chromatography and mass spectrometry, to analyze and compare the metabolic profiles of these two sources, identifying a total of 285 components.
  • Key findings revealed 43 distinct components between the sources, with specific marker compounds for each, suggesting significant differences in their chemical composition that can aid in quality control and tailored clinical use of Dragon's Blood.
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  • Chronic allograft vasculopathy (CAV) is a major cause of late organ transplant failure, highlighting the need for new treatment options to combat its progression.
  • In an experiment using mice, researchers compared the effects of human mesenchymal-like endometrial regenerative cells (ERCs), particularly those modified to secrete interleukin-37 (IL-37), against control groups.
  • The results showed that IL-37-secreting ERCs significantly reduced harmful vascular changes and inflammation, inhibited certain T cell responses, lowered donor-specific antibody production, and increased regulatory T cell (Treg) levels, suggesting a potential therapeutic role for these cells in managing CAV.
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To accurately identify the metabolites is crucial in a number of research fields, and discovery of new compounds from the natural products can benefit the development of new drugs. However, the preferable phytochemistry or liquid chromatography/mass spectrometry approach is time-/labor-extensive or receives unconvincing identifications. Herein, we presented a strategy, by integrating offline two-dimensional liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (2D-LC/IM-QTOF-MS), exclusion list-containing high-definition data-dependent acquisition (HDDDA-EL), and quantitative structure-retention relationship (QSRR) prediction of the retention time (t), to facilitate the in-depth and more reliable identification of herbal components and thus to discover new compounds more efficiently.

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Ginseng extracts are extensively used as raw materials for food supplements and herbal medicines. This study aimed to characterize ginsenosides obtained from six plant extracts (, red ginseng, , , , and var. ) and compared them with their metabolic profiles mediated by rat intestinal microbiota.

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Background: The disruption of intestinal barrier functions and the dysregulation of mucosal immune responses, mediated by aberrant purinergic metabolism, are involved in the pathogenesis of inflammatory bowel diseases (IBD). A novel mesenchymal-like endometrial regenerative cells (ERCs) has demonstrated a significant therapeutic effect on colitis. As a phenotypic marker of ERCs, CD73 has been largely neglected for its immunosuppressive function in regulating purinergic metabolism.

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Purpose: In the treatment of early-stage rectal cancer, a growing number of studies have shown that transanal endoscopic microsurgery is one of the alternatives to radical surgery adhering to total mesorectal excision that can reduce the incidence of adverse events without compromising treatment outcomes. The purpose of this meta-analysis is to compare the safety and treatment effect of transanal endoscopic microsurgery and radical surgery adhering to total mesorectal excision to provide a basis for clinical treatment selections.

Method: We searched the literatures of four major databases, PubMed, Embase, Web of science, and Cochrane Library, without limitation of time.

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Background: IL-37 is a recently identified cytokine with potent immunosuppressive functions. The research fronts of IL-37 are worth investigating, and there is no bibliometric analysis in this field. The purpose of this study is to construct the intellectual base and predict research hotspots of IL-37 research both quantitatively and qualitatively according to bibliometric analysis.

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Background: Immunotherapy has been considered as a promising cancer treatment for hepatocellular carcinoma (HCC). However, due to the particular immune environment of the liver, identifying patients who could benefit from immunotherapy is critical in clinical practice.

Methods: The pyroptosis gene expression database of 54 candidates from The Cancer Genome Atlas (TCGA) were collected to discover the critical prognostic-related pyroptosis genes.

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  • This study reviews the global collaboration in research on mesenchymal stem cells (MSCs) related to liver diseases using bibliometrics to assess trends and knowledge structures from 2001-2021.
  • A total of 3,404 publications were analyzed, showing an increase in MSC research, primarily from institutions in China and the USA, with Li L being the most prolific author.
  • Key emerging topics identified include liver scaffolds, MSC therapy, and keywords like "machine perfusion" and "microRNAs," providing insights for future research directions.
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Challenges encountered in plant metabolites characterization by liquid chromatography/mass spectrometry can arise from the insufficient chromatography separation, the lack of specific database, and low reliability of identification because of the ubiquitous isomerism. Herein, we present an integral approach, by combining comprehensive off-line two-dimensional liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (2D-LC/IM-QTOF-MS), automatic peak annotation, molecular networking, and collision cross section (CCS) prediction, aimed to improve the resolution and reliability in MS-oriented metabolites characterization. Using the seeds of Cuscuta chinensis as a case, the configuration of an XBridge Amide column of hydrophilic interaction chromatography (HILIC) and a Zorbax SB-Aq column of reversed-phase chromatography (RPC), in an off-line mode, showed the orthogonality of 0.

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Background: The prognostic value of m6A-related genes in hepatocellular carcinoma (HCC) and its correlation with the immune microenvironment still requires further investigation.

Methods: Consensus clustering by m6A related genes was used to classify 374 patients with HCC from The Cancer Genome Atlas (TCGA) database. Then we performed the least absolute shrinkage and selection operator (LASSO) to construct the m6A related genes model.

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Liquid chromatography/mass spectrometry (LC/MS) is extensively applied for the untargeted/targeted analyses of the herbal components, utilizing data-dependent acquisition (DDA) or data-independent acquisition (DIA) to record the fragmentation information useful for the structural elucidation. A new trend recently has emerged by integrating DDA and DIA to render the hybrid scan, which, unfortunately, has rarely been reported. Herein, by using the Vion™ ion-mobility quadrupole time-of-flight mass spectrometer, a hybrid scan strategy (HDMS-HDDDA) was presented and validated by the untargeted characterization of the multicomponents from Carthamus tinctorius (safflower), in combination with reversed-phase ultra-high performance liquid chromatography (RP-UHPLC).

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Article Synopsis
  • * Advances in analytical technologies, such as multi-dimensional chromatography and mass spectrometry, have significantly improved ginseng analysis, enabling systematic and personalized approaches.
  • * The review focuses on new compounds, quality control, and biosynthesis pathways of ginseng, while also discussing future challenges and trends in ginseng research.
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Data-dependent acquisition (DDA) and data-independent acquisition (DIA)-based MS strategies are extensively applied in metabolites characterization. DDA gives accurate MS information, but receives low coverage, while DIA covers the entire mass range, but the precursor-product ions matching often yields false positives. Currently available MS scan approaches rarely integrate DIA and DDA within a duty circle.

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The role of polysaccharides in quality control of ginseng is underestimated. Large-scale comparison on the polysaccharides of Panax ginseng (PG), P. quinquefolius (PQ), P.

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Recent studies in patients with spinal cord injuries (SCIs) have confirmed the diagnostic potential of biofluid-based biomarkers, as a topic of increasing interest in relation to SCI diagnosis and treatment. This paper reviews the research progress and application prospects of recently identified SCI-related biomarkers. Many structural proteins, such as glial fibrillary acidic protein, S100-β, ubiquitin carboxy-terminal hydrolase-L1, neurofilament light, and tau protein were correlated with the diagnosis, American Spinal Injury Association Impairment Scale, and prognosis of SCI to different degrees.

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Objective: This study aimed to discover the ceRNAs network in the pathophysiological development of human colorectal cancer (CRC) and to screen biomarkers for target therapy and prognosis by using integrated bioinformatics analysis.

Methods: Data on gene expressions of mRNAs, miRNAs, and circRNAs and clinical information were downloaded from The Cancer Genome Atlas and Gene Expression Omnibus databases, respectively. Differentially expressed mRNAs (DEmRNAs) were identified by using the DESeq2 package of R software.

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Article Synopsis
  • Chronic allograft vasculopathy (CAV) is a significant issue in transplant survival, with various complicated mechanisms hindering effective treatments.
  • The study investigates the combined effects of melatonin (MT) and mesenchymal stromal cells (MSCs) on mitigating CAV in a mouse model of aorta transplantation.
  • Results indicate that the MT and MSC combination significantly reduces CAV-related pathological changes and alters immune cell infiltration, promoting a healthier immune response by increasing regulatory T cells (Tregs) while inhibiting other T cell populations.
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Inthomycins belong to a growing family of oxazole-containing polyketides and exhibit a broad spectrum of anti-oomycete and herbicidal activities. In this study, we purified inthomycins A and B from the metabolites of sp. strain SYP-A7193 and determined their chemical structures.

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Article Synopsis
  • The study investigates in-source fragmentation of ginsenosides in positive ESI mode (pISF-G), which presents challenges for identifying their structures and emphasizes the need for understanding this process for better Ginseng quality control.
  • Using advanced high-resolution mass spectrometry techniques, researchers were able to identify specific ion clusters from protonated sapogenins, which helped classify different types of ginsenosides.
  • Through chemometric analysis of chromatogram data, 35 marker compounds were identified that enabled the differentiation of various Ginseng species and compound formulas, highlighting the potential for enhanced Ginseng authentication.
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