Identification, Biological Characteristics, and Active Site Residues of 3-Ketosteroid Δ-Dehydrogenase Homologues from .

3-Ketosteroid Δ-dehydrogenase (KsdD) is the key enzyme responsible for Δ-dehydrogenation, which is one of the most valuable reactions for steroid catabolism. has been widely used in the industry due to its superior bioconversion efficiency, but KsdD information is not yet fully clear. Here, five KsdD homologues were identified in CGMCC 14539.