Single-cell transcriptomics of blood identified IFIT1 neutrophil subcluster expansion in NTM-PD patients.

Objective: Non-tuberculous mycobacterial pulmonary disease (NTM-PD) is caused by an imbalance between pathogens and impaired host immune responses. Mycobacterium avium complex (MAC) and Mycobacterium abscessus (MAB) are the two major pathogens that cause NTM-PD. In this study, we sought to dissect the transcriptomes of peripheral blood immune cells at the single-cell resolution in NTM-PD patients and explore potential clinical markers for NTM-PD diagnosis and treatment.

Circular RNA circTRAPPC6B Enhances IL-6 and IL-1β Expression and Repolarizes Induced Macrophages from M2- to M1-Like Phenotype by Targeting miR-892c-3p.

() infection elicits macrophage polarization into M2 phenotype to block the host's protective immune response. However, it remains unclear how regulates macrophage polarization. Recent studies have suggested that noncoding RNA may play a role in macrophage polarization.

Circular RNA TRAPPC6B inhibits intracellular growth while inducing autophagy in macrophages by targeting microRNA-874-3p.

Objectives: Genetic and epigenetic mechanisms regulate antimicrobial immunity against (Mtb) infection.

Methods: The present study assessed circular RNA TRAPPC6B (circTRAPPC6B) for antimicrobial immune functions and defined mechanisms wherein circTRAPPC6B regulates Mtb growth, autophagy and microRNA in macrophages.

Results: The Mtb infection of monocytes/macrophages resulted in a significantly decreased level of circTRAPPC6B that inhibited intracellular Mtb growth in macrophages.

["Multi-component-multi-target-multi-pathway" mechanism of Kuihua Hugan Tablets based on network pharmacology].

To predict the targets of active ingredients of Kuihua Hugan Tablets by network pharmacology, and explore the "multi-component-multi-target-multi-pathway" hepatoprotective mechanism of action. First, through traditional Chinese medicine systems pharmacology(TCMSP) and TCM Database@Taiwan Database, main active ingredients of Kuihua Hugan Tablets were screened out based on oral bioavailability(OB), drug-likeness(DL) and effective half-lives(HL). The targets of active ingredients of Kuihua Hugan Tablets were predicted based on the PharmMapper method.

Protective Effect of Norcantharidin on Collagen-Induced Arthritis Rats.

Objective: To observe the effect of norcantharidin (NCTD) on collagen-induced arthritis (CIA) rats.

Methods: Sixty Sprague-Dawley(SD) rats were randomly divided into 6 groups (n=10): normal group, CIA model group(model group), NCTD low-dose group [1.35 mg/(kg•d)], NCTD middle-dose group [2.

[Protective effect of acupuncture on heart in mice with hyperlipemia and its mechanism].

Objective: To observe the inhibiting effect of acupuncture on blood lipid, myocardial hypertrophy and fibrosis in mice with hyperlipemia, and explore its possible action mechanism.

Methods: Ten inbred mice (C57) were applied. Forty ApoE(-/-) mice who removed gene of apolipoprotein E were randomly divided into a control group, a non-acupoint group, an acupoint group and a medication group.

Effects of cold-damp and hot-damp environment on VEGF and IL-1 expression in joint cartilage cells in adjuvant arthritis in rats.

Objective: To study the effects of environmental factors on the degree of injury and expression of vascular endothelial growth factor (VEGF) and interleukin-1 (IL-1) in cartilage cells of the joint in a rat model of adjuvant arthritis (AA).

Methods: SD rats aged 10 months were randomly divided into 4 groups that varied by temperature and humidity housing conditions and induction of AA: a control group, a model group, a cold-damp group, and a hot-damp group. All groups except the control group were induced with AA.

Assessment of clinical effect of therapy combining disease with syndrome on rheumatoid arthritis.

Objective: To observe the effectiveness and safety of a therapy combining disease with syndrome on rheumatoid arthritis.

Methods: Eighty patients with rheumatoid arthritis belonging to syndrome of damp-heat obstruction were randomly divided into a treatment group and a control group according to stratified blocked randomization method. Forty cases in the control group orally took Loxoprofen Sodium Tablet and Leifumite Tablet and the other 40 cases in the treatment group orally took a Chinese medicine for 12 weeks as a course of treatment.