[Effect and Mechanism of Uric Acid in Regulating Larval Growth and Development of ].

Objective To explore the effect and mechanism of uric acid (UA) in regulating the larval growth and development of . Methods A total of 1350 newly hatched first-instar larvae of wild-type (W1118) were collected,and the model of hyperuricemia was constructed with a high purine diet.The larvae were assigned into three groups (=150):control (standard corn meal medium),low-dose adenine (corn meal medium containing 0.

[Effect of High-Concentration Uric Acid on Nitric Oxide].

Uric acid (UA) is the final product of purine metabolism in human body,and its metabolic disorder will induce hyperuricemia (HUA).The occurrence and development of HUA are associated with a variety of pathological mechanisms such as oxidative stress injury,activation of inflammatory cytokines,and activation of renin-angiotensin-aldosterone system.These mechanisms directly or indirectly affect the bioavailability of endogenous nitric oxide (NO).

Luteolin Protects Pheochromocytoma (PC-12) Cells against A -Induced Cell Apoptosis through the ER/ERK/MAPK Signalling Pathway.

The regulatory effect of luteolin on the progression of Alzheimer's disease (AD) remains unclear from the perspective of apoptosis. The present study aimed to investigate the protective effects of luteolin against A -induced cell apoptosis in pheochromocytoma (PC-12) cells. A was used to induce an in vitro model of AD.

[Expressions of HO-2 and CO in the corpus cavernosum of castrated rats].

Objective: To explore the expressions of HO-2 and CO in the corpus cavernosum of castrated rats in order to further study the pathogenesis of erectile dysfunction (ED).

Methods: We randomly divided 72 male SD rats into four groups: normal control, sham operation, castration, and castration + ZnPP. We detected intracavernous pressure (ICP) and penile erection in the basic condition and after apomorphine (APO) induction, determined the expression of the HO-2 protein in the corpus cavernosum by laser scanning confocal microscopy, and measured the level of CO by spectrophotometry during different periods of penile erection.

Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons.

Purpose: To investigate the mechanism of the anti-epileptic effect of Ganoderma lucidum polysaccharides (GLP), the changes of intracellular calcium and CaMK II α expression in a model of epileptic neurons were investigated.

Method: Primary hippocampal neurons were divided into: 1) Control group, neurons were cultured with Neurobasal medium, for 3 hours; 2) Model group I: neurons were incubated with Mg(2+) free medium for 3 hours; 3) Model group II: neurons were incubated with Mg(2+) free medium for 3 hours then cultured with the normal medium for a further 3 hours; 4) GLP group I: neurons were incubated with Mg(2+) free medium containing GLP (0.375 mg/ml) for 3 hours; 5) GLP group II: neurons were incubated with Mg(2+) free medium for 3 hours then cultured with a normal culture medium containing GLP for a further 3 hours.

[Risk factors related to infantile spastic cerebral palsy among 145 cases].

Objective: To study the risk factors related to spastic type of cerebral palsy through a screening program.

Methods: With a 1:2 matched case-control study design, 145 cases were selected from the Cerebral Palsy Treatment and Management Center in Heilongjiang Province from Dec., 2007 through May 2009.