cAMP-dependent protein kinase activated Fyn in spinal dorsal horn to regulate NMDA receptor function during inflammatory pain.

Selective inhibition of GluN2B-containing NMDA receptor (GluN2BR) in spinal dorsal horn effectively alleviates inflammatory pain, suggesting the up-regulation of GluN2BR function involved in central sensitization. Previous studies have demonstrated that the increase in GluN2BR synaptic expression serves as a key step to enhance GluN2BR function after intradermal injection of Complete Freund's Adjuvant (CFA). Here, we showed that cAMP-dependent protein kinase (PKA) played an important role in redistributing GluN2BR at synapses, because inhibition of PKA activity impaired GluN2BR accumulation at post-synaptic density (PSD)-enriched fraction in CFA-injected mice, and direct stimulation of PKA in naïve mice mimicked the effect of CFA by recruiting GluN2BR at PSD fraction to evoke pain sensitization.

Isoflavone regulates lipid metabolism via expression of related genes in OVX rats fed on a high-fat diet.

Objective: To investigate the effects of isoflavone on body weight, fat mass, and gene expression in relation to lipid metabolism.

Methods: Thirty-six female SD rats were ovariectomized or sham-operated and fed on a high-fat diet. Two months later, abdominal incision was made, blood was collected to separate serum, and the liver and adipose tissue were immediately collected and weighed.

[Effect of apoptosis in human breast cancer cells and its probable mechanisms by genistein].

Objective: To investigate the effects of genistein on human breast cancer cell MCF-7 apoptosis and its probable mechanisms.

Methods: In this study, the methods of MTT, cell apoptosis detecting in fluorescent and electronic microscope and flow cytometry, and expression of Bax and erbB-2 protein were employed.

Results: The results showed that genistein could significantly inhibit the growth and induce the apoptosis of MCF-7 cells.

Blocking effects of genistein on cell proliferation and possible mechanism in human gastric carcinoma.

Aim: To study the blocking effects of genistein on cell proliferation cycle in human gastric carcinoma cells (SGC-7901) and the possible mechanism.

Methods: MTT assay was applied in the detection of the inhibitory effects of genistein on cell proliferation. Flow cytometry was used to analyze the cell cycle distribution.