Analysis of pregnancy and neonatal outcomes in 100 pregnant women with Rh-negative blood type.

Background: This study aimed to explore variations in prenatal care, delivery methods, influencing factors, and neonatal outcomes among Rh-negative pregnant women, so as to improve pregnancy healthcare for this demographic, raise the quality of maternal-fetal management, and safeguard the health of both mother and infant.

Methods: This study included 200 women who received routine prenatal care, exhibited no other pregnancy complications, and were admitted for delivery. They were divided into an observation group (100 Rh-negative blood type) and a control group (100 Rh-positive blood type).

Glycosparvine A, a new indole alkaloid from the leaves of .

In the course of our ongoing search for biologically active compounds from medicinal plant, the ethyl acetate extract from the leaves of (Rutaceae) was investigated. Six compounds including a new indole alkaloid, glycosparvine A (), and five previously known metabolites (-) were identified. The structure of the new compound () was unambiguously elucidated by spectroscopic analyses, including 1D/2D NMR spectroscopy and HRESIMS.

Bioactive sulfur-containing amides from the leaves of .

Two new sulphur-containing amides, glylucidamides C-D (-), along with twelve known analogues (-) were isolated and characterised from the leaves of . The chemical structures of and were elucidated by extensive spectroscopic methods and the known compounds were identified by comparisons their data with those reported in the literatures. All new compounds were evaluated for their anti-inflammatory activities examining the inhibitory activity on nitric oxide (NO) production induced by lipopolysaccharide in mouse macrophage RAW 264.

[Ketogenic diet improves low temperature tolerance in mice by up-regulating PPARα in the liver and brown adipose tissue].

The aim of the present study was to investigate the effects of short-term ketogenic diet on the low temperature tolerance of mice and the involvement of peroxisome proliferator-activated receptor α (PPARα). C57BL/6J mice were divided into two groups: normal diet (WT+ND) group and ketogenic diet (WT+KD) group. After being fed with normal or ketogenic diet at room temperature for 2 d, the mice were exposed to 4 °C low temperature for 12 h.

Consensus of MASs With Input and Communication Delays by Predictor-Based Protocol.

In this article, the consensus problem of multiagent systems (MASs) affected by input and communication delays is investigated. A predictor-based state feedback protocol is used to reach the consensus of linear MASs by delay compensation. In order to analyze the maximum delay under the predictor-based protocol, the overall MASs are equivalent to the feedback interconnection system, including a linear time-invariant system and a time-delay operator, in view of the characteristic of the Laplacian matrix.

[Comparison of Plasma Components between Frozen Plasma and Fresh Frozen Plasma].

Objective: To compare the plasma components of frozen plasma (FP) and fresh frozen plasma (FFP).

Methods: Twenty samples of FP and 20 samples of FFP from Beijing Red Cross Blood Center were randomly selected. Immediately after plasma melting, 12 plasma components including coagulation factor, fibrinolytic system and anticoagulation protein were detected, including activated partial thromboplastin time (APTT), prothrombin time (PT), coagulation factor Ⅷ (FⅧ) activity, coagulation factor Ⅴ (FⅤ) activity, fibrinogen(FIB) level, ADAMTS-13 activity, von Willebrand factor(vWF) activity, D-dimer (D-dimer, DD), fibrin degradation products (FDP), antithrombin (AT), protein C (PC), and protein S (PS).

Prognosis of Neurological Improvement in Inpatient Acute Ischemic Stroke Survivors: A Propensity Score Matching Analysis.

Objectives: Stroke has become a national concern in China. Early prediction of stroke benefits patients and aids medical professionals in clinical decision making and rehabilitation plans to improve successful outcomes. To identify prediction factors influencing short-term outcomes in patients with acute ischemic stroke (AIS).

Time-restricted feeding alleviates cardiac dysfunction induced by simulated microgravity via restoring cardiac FGF21 signaling.

Dietary restriction has been well-described to improve health metrics, but whether it could benefit pathophysiological adaptation to extreme environment, for example, microgravity, remains unknown. Here, we investigated the effects of a daily rhythm of fasting and feeding without reducing caloric intake on cardiac function and metabolism against simulated microgravity. Male rats under ad libitum feeding or time-restricted feeding (TRF; food access limited to 8 hours every day) were subjected to hindlimb unloading (HU) to simulate microgravity.

[Analysis of the Irregular Antibodies of the Patients and Its Clinical Significance].

Objective: To retrospectively analyze the identification results of irregular antibodies, to clarify the distribution features and to explore the relation of alloantibodies and autoantibodies with the immunized history of patients and disease kinds.

Methods: 49 820 patients who applied for red blood transfusion during Sep 1st 2017 to Sep 1st 2018 were selected. All the specimens were screened for the antibody by microcolumn gel antiglobulin technique, which then were identified for irregular antibody.

[Genomics-based Biomarkers for Diseases:A Bibliometric Analysis Based on Web of Science].

To analyze the research hotspots and trends of biomarkers for diseases based on genomics and thus provide basis for the future studies in this field. Based on the Web of Science,we analyzed the genomics-based biomarkers for diseases in literature published between 2006 and 2018 in terms of country and institutions,knowledge base,research hotspots,and trends by using bibliometric methods and CiteSpace software. A total of 998 articles were retrieved.

[Effect of HIF-1α on Angiogenesis-Related Factors in K562 Cells].

Objective: To explore the mechanisms of angiogenesis in chronic myeloid leukemia (CML) through detecting the levels of angiogenesis-related factors secreted from K562 cells after overexpression and interference of HIF-1α gene in K562 cells.

Methods: The K562 cells were transfected by lentiviruses carried and interfered HIF-1α gene, then the transtected K562 cells with carried and interfered with HIF-1α gene were enrolled in overexpression and interference groups respectively, at the same time the K562 cells transfected by the empty virus were enrolled in control group. The cells were harvested after culture for 72 hours under normoxid condition.

[A modified protocol for generating the simulated weightlessness rat model].

Objective: To introduce a modified protocol for generating the simulated weightlessness rat model by hindlimb unloading.

Methods: Ninety male adult SD rats were randomly divided into three groups: the control group, classical suspension group and modified suspension group (n=30/group). In the classical suspension group, a strip of medical adhesive tape was attached to the tail, with horizontal filament tape wrapping.

[miR-494-3p reduces insulin sensitivity in diabetic cardiomyocytes by down-regulation of insulin receptor substrate 1].

More and more evidence suggests that microRNA is widely involved in the regulation of cardiovascular function. Our preliminary experiment showed that miR-494-3p was increased in heart of diabetic rats, and miR-494-3p was reported to be related to metabolism such as obesity and exercise. Therefore, this study was aimed to explore the role of miR-494-3p in diabetic myocardial insulin sensitivity and the related mechanism.

Material and microbial changes during corn stalk silage and their effects on methane fermentation.

Silage efficiency is crucial for corn stalk storage in methane production. This study investigated characteristics of dynamic changes in materials and microbes during the silage process of corn stalks from the initial to stable state. We conducted laboratory-scale study of different silage corn stalks, and optimized silage time (0, 2, 5, 10, 20, and 30days) for methane production and the endogenous microbial community.

Paradoxical Elevation of High Density Lipoprotein Cholesterol in Association with Lacunar-Type Cerebral Infarction.

Background: The aim of this study was to evaluate the association between high-density lipoprotein cholesterol (HDLC) levels and the risk of lacunar infarction (LI) in a retrospective cohort study in China.

Material And Methods: We recruited 229 patients with obsolete brain infarctions single side (SOBI), 218 with obsolete brain infarctions bilateral sides (BOBI), 193 with both acute stroke and obsolete lacunar infarctions single side (AI&SOBI), 113 with both acute stroke and obsolete lacunar infarctions bilateral sides (AI&BOBI), and 203 without any infarctions (Control).

Results: 1) The plasma levels of HDLC in group BOBI, AI&SOBI, and AI&BOBI were higher than in the control group, and lower in group SOBI than in the control group (p<0.

Economic design in a long-distance migrating molluscivore: how fast-fuelling red knots in Bohai Bay, China, get away with small gizzards.

We carried out an observational and experimental study to decipher how resource characteristics, in interaction with the predator's phenotype, constrain a fitness-determining performance measure, i.e. refuelling in a migrant bird.

Diversity of endophytic fungi from roots of Panax ginseng and their saponin yield capacities.

Endophytes of medicinal plants have the capacity to synthesis same or similar active substances with their hosts. To investigate the diversity and capacity to produce saponins of endophytic fungi of Panax ginseng, thirty-eight strains of were isolated. Polymerase chain reaction (PCR) and sequencing were used to identify the isolates, and saponins concentrations in the cultures were measured.

[Reconstruction of facial soft tissue defects with free flaps by microsurgery].

Objective: To investigate the method and indications for reconstruction of facial complicated soft tissue defects with free flaps by microsurgery.

Methods: 37 patients (16 males and 21 females, aged from 1 to 54 years) with different size of facial soft tissue defects were reconstructed with free flaps, including 10 latissimus dorsi myocutaneous flaps, 3 thoracodorsal artery perforator flaps, 9 scapular flaps, forearm flaps and 9 postauricular flaps. The defects size ranged from 1 cm x 2 cm to 25 cm x 12 cm.

Chinese medicine for menopausal syndrome: current status, problems and strategies.

The use of Chinese medicine (CM) for the management of: menopausal syndrome is considered effective both at home and abroad, and more and more clinical studies are confirming its efficacy. However, many problems still exit in current studies, such as the standard of CM syndrome differentiation, the design methodology and criteria to assess the quality of clinical trials and the efficacy of interventions. In this paper, the authors present the CM research and treatment strategies for menopausal syndrome with concepts explaining the CM understanding of the mechanism of the disorder.

Selection and characteristics of a switchgrass-colonizing microbial community to produce extracellular cellulases and xylanases.

A microbial community was selected for growth on dried and NaOH-treated switchgrass. During a 14-day liquid cultivation, a 70% loss in dry weight was observed during the first 4 days and after 14 days, the hemicellulose and cellulose in the system were degraded by 73.5% and 67.

[Study on the specific immunity of dendritic cell vaccine loading human umbilical vein endothelial cell antigen against U14 cervical cancer].

Aim: To explore the specific immunity of dendritic cell (DC) vaccine loading human umbilical vein endothelial cell (HUVEC) antigen.against U14 cervical cancer of mice.

Methods: Primary HUVECs were cultured, identified and made into antigen.

[Pretest survey on the Chinese menopause rating scale].

Objective: To select the items from the Chinese menopause rating scale (CMRS) through pre-testing those people with menopausal syndromes.

Methods: 293 people were surveyed in Guangzhou in 2005, among which 196 people with menopausal syndromes and others without. Psychometrics methods were employed to develop the scale.