A novel survival rat model of hyperkalemia and landiolol induced cardioplegic arrest and resuscitation via cardiopulmonary bypass.

Objective: A small animal model would be an effective tool for research on the pathophysiology of cardiopulmonary bypass (CPB). However, numerous CPB models do not involve myocardial arrest and resuscitation. The aim of this research is to establish an easily achievable myocardial arrest and resuscitation CPB model through hyperkalemia and landiolol, simulating clinical cardiac surgery.

Meplazumab in hospitalized adults with severe COVID-19 (DEFLECT): a multicenter, seamless phase 2/3, randomized, third-party double-blind clinical trial.

Meplazumab, a humanized CD147 antibody, has shown favourable safety and efficacy in our previous clinical studies. In DEFLECT (NCT04586153), 167 patients with severe COVID-19 were enroled and randomized to receive three dosages of meplazumab and a placebo. Meplazumab at 0.

Risk Factors Analysis and the Establishment of Nomogram Prediction Model of Hidden Blood Loss After Total Hip Arthroplasty for Femoral Neck Fracture in Elderly Women.

Purpose: To investigate the risk factors of increased hidden blood loss (HBL) after total hip arthroplasty (THA) for femoral neck fracture in elderly women and establish the nomogram prediction model for the guidance to reduce HBL in clinic.

Patients And Methods: A total of 206 elderly female patients with femoral neck fracture who underwent THA in Northern Jiangsu People's Hospital from January 2019 to November 2021 were included. The demographic and relevant clinical information of the patients were collected.

[Efficacy and safety of low-intensity extracorporeal shock wave therapy in the treatment of ED: A meta-analysis of randomized controlled trials].

Objective: To evaluate the clinical efficacy and safety of low-intensity extracorporeal shock wave therapy (LI-ESWT) in the treatment of ED based on the available clinical evidence.

Methods: We searched PubMed, MEDLINE, EMBASE, Cochrane Library, CNKI, VIP, CBM and Wanfang Database up to June 2018 for published randomized controlled trials on the treatment of ED by LI-ESWT. We performed literature screening, data extraction and quality evaluation according to inclusion and exclusion criteria, and conducted a meta-analysis of the data obtained using the RevMan 5.

[Treatment Effect and Ecological Risk Assessment of Typical Antibiotics in Wastewater from Large-scale Dairy Farms in Tianjin].

To obtain the content level and treatment efficiency of typical antibiotics in wastewater treatment in large-scale dairy farms in Tianjin, the SPE-UPLC-MS/MS (ultra-high performance liquid chromatography tandem triple quadruple mass spectrometry combined with solid-phase extraction for pretreatment) technology was utilized to investigate and monitor seven typical antibiotics in wastewater from 12 large-scale dairy farms in Tianjin. Antibiotic residues were detected in 12 large-scale dairy farms before and after wastewater treatment. In the wastewater before treatment, the detection rates of tilmicosin (TIL), oxytetracycline hydrochloride (OTC), sparfloxacin (SPA), sulfathiazloe (STZ), ofloxacin (OFL), and sarafloxacin hydrochloride (SAR) were all 100%, whereas the detection rate of sulfadiazine (SDZ) was 83.

Synthesis of the ABC Tricyclic System of Daphnicyclidin A.

A substrate-stereocontrolled synthesis of the ABC tricyclic system of daphnicyclidin A is developed. The key reactions include an efficient tandem N-allylation-S2' reaction to assemble 2,3,4-cis trisubstituent pyrrolidine ring C and two intramolecular Horner-Wadsworth-Emmons reactions to construct cycloheptanone ring A and piperidine ring B.

Synthesis of the BCD Tricyclic Core of Densanins A and B.

A substrate stereocontrolled synthesis of the BCD tricyclic ring system of densanins A and B has been developed. The key transformations include the assembling of ring B via an unprecedented tandem N-allylation/SN2' reaction and the construction of ring C via gold-catalyzed alkenylation of terminal alkyne and pyrrole.

[Effects of ulinastatin on cognitive function in patients with coronary artery bypass grafting].

Objective: To investigate the effects of ulinastatin(UTI) on postoperative cognitive function in patients undergoing coronary artery bypass grafting.

Methods: One hundred and twenty-seven patients undergoing elective coronary artery bypass surgery were randomly divided into three groups:high-dose UTI group(16000 U/kg i.v.

[Qualitative analysis of platelet rich plasma prepared by acute plateletpheresis in patients undergoing heart surgery].

This study was aimed to evaluate the efficiency and effectiveness of platelet-rich plasma(PRP) prepared by acute plateletpheresis in patients undergoing open heart surgery, and to analyze the quality of prepared platelet-rich plasma. Whole blood from 20 patients with ASAII-III was collected and PRP was harvested by machine after induction of anesthesia. Platelet count (Plt), mean platelet volume (MPV), platelet distribution width (PDW), plasma pH, plasma lactic acid (LA) concentration, and lactic dehydrogenase (LDH) concentration, germiculture result, CD62p and PAC-1 positive rate of inactivated and activated platelets by ADP in the whole blood before plateletpheresis (T1) , in the PRP after plateletpheresis (T2) and PRP before back-transfusion (T3) were determinated.

Prognostic value of late gadolinium enhancement in dilated cardiomyopathy patients. A meta-analysis.

Objective: To evaluate the prognostic value of late gadolinium enhancement (LGE) in dilated cardiomyopathy (DCM) patients.

Methods: We searched PubMed, MEDLINE, the Cochrane library and EMBASE databases from September to December 2012 in the Renmin Hospital of Wuhan University, Wuhan, China for studies of LGE in DCM patients. We extracted the clinical outcomes (all-cause mortality, cardiovascular mortality, sudden cardiac death [SCD], aborted SCD, heart failure hospitalization) after carefully reviewed.

Use of hypophysin in hypotensive patients with low systemic vascular resistance following cardiopulmonary bypass.

Objective: This study aimed to test the effects of hypophysin on hemodynamics and coronary artery caliber of patients with hypotension and decreased systemic vascular resistance (SVR) following cardiopulmonary bypass (CPB).

Methods: Twenty-four patients with mean arterial pressure (MAP) < 60 mmHg, mean aorta pressure < 70 mmHg, SVR < 800 dynes.sec.

Baicalein, a natural product, selectively activating AMPKα(2) and ameliorates metabolic disorder in diet-induced mice.

The aim of the present study was to determine the effect of baicalein on metabolic syndrome induced by a high-fat diet in mice. The mice developed obesity, dyslipidemia, fatty liver, diabetes and insulin resistance. These disorders were effectively normalized in baicalein-treated mice.

[The protective effects of insulin on cardiocyte apoptosis challenged by burn serum].

Objective: To investigate the protective effects of insulin on burn serum-challenged cardiocyte apoptosis and its mechanism.

Methods: Burn-serum challenged cardiocytes were pretreated with insulin and inhibitors to pathway SB203580 and LY294002. The expression of cardiac myofilament proteins cleaved-caspase-3, Bax and phosphorylation nuclear factor-ΚB inhibitive factor α (p-IΚBα) were examined by Western blotting.

[Intensive insulin treatment protected the cardiac myocytes against apoptosis in severely scalded rats].

Objective: To investigate the effect of intensive insulin treatment, in the protection of myocardiocytes against apoptosis in severely scalded rats and its underlying mechanism.

Methods: Eighteen Sprague-Dawley (SD) rats were randomly divided into three groups (6/each) to receive: sham surgery, burn damage (on the back of the animals, degreeIII, to 30% of total body surface area), and burn damage+intensive insulin treatment. Tissue samples were collected from the left ventricle 6 hours after infliction of the burn damage for the examination of myocardial cell apoptosis [by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) staining] and the expression of apoptosis-related molecules caspase-3, Bax, and Bcl-2 (by immuno-histochemistry and Western blotting).

Two-dimensional strain combined with adenosine stress echocardiography assessment of viable myocardium.

The objective of this study was to explore a new method for the identification of viable myocardium by means of two-dimensional (2D) strain imaging combined with adenosine stress echocardiography. A total of 15 anesthetized open-chest healthy mongrel dogs underwent left anterior descending coronary artery occlusion for 90 min followed by 120-min reperfusion. Adenosine was infused at 140 μg kg(-1) min(-1) over a period of 6 min.

[Surgical treatment of post-infarction left ventricular aneurysm: clinical analysis].

Objective: To explore the clinical experiences, efficacies and postoperative left ventricular remodeling changes of surgical ventricular reconstruction in the treatment of post-infarction left ventricular aneurysm.

Methods: The investigators reviewed retrospectively the clinical data, operative approaches and follow-up outcomes of consecutive 194 patients with post-infarction left ventricular aneurysm, who underwent surgical ventricular reconstruction between January 1997 and December 2009. There were 54 cases in the linear group and 137 cases in the endoventricular patch plasty group.

[Evaluation of viable myocardium by two-dimensional strain imaging combined with adenosine stress echocardiography in dogs underwent experimental ischemia/reperfusion injury].

Objective: to explore the feasibility of evaluating viable myocardium with two-dimensional strain imaging combined with adenosine stress echocardiography.

Methods: acute myocardial infarction and reperfusion model was made by ligating anterior descending coronary artery for 90 minutes followed by 120-minute reperfusion in 15 healthy mongrel dogs. Images were acquired at baseline and after reperfusion.

Comparative study of on-pump and off-pump coronary bypass surgery in patients with triple-vessel coronary artery disease.

Background: Studies on selected patients undergoing off-pump versus on-pump coronary artery bypass surgery have produced inconsistent results, especially in patients with multiple coronary artery disease. This study compared the clinical results of on-pump and off-pump coronary bypass surgery in patients with triple-vessel disease.

Methods: A total of 300 consecutive isolated, multiple coronary artery bypass grafting (CABG) patients were assigned to the off-pump coronary artery bypass (OPCAB, n = 150) or CABG with cardiopulmonary bypass (CCABG, n = 150) groups.