Analysis of mechanisms of Shenhuang Granule in treating severe COVID-19 based on network pharmacology and molecular docking.

Objective: Severe cases of coronavirus disease 2019 (COVID-19) are expected to have a worse prognosis than mild cases. Shenhuang Granule (SHG) has been shown to be a safe and effective treatment for severe COVID-19 in a previous randomized clinical trial, but the active chemical constituents and underlying mechanisms of action remain unknown. The goal of this study is to explore the chemical basis and mechanisms of SHG in the treatment of severe COVID-19, using network pharmacology.

[Carbonized Apple Branches Decrease the Accumulation and Damage of Cadmium on Apple Rootstock by Reducing DTPA-Cd in Soil].

To explore the effects of carbonized apple branches on cadmium(Cd) accumulation and its damage to apple rootstock, the rootstocks of apple( Rehd.) in pots containing soil together with 0.5% and 1%() carbonized apple branches were irrigated by a nutrient solution containing CdSO.

Hydrogen Sulfide Alleviates Alkaline Salt Stress by Regulating the Expression of MicroRNAs in Rehd. Roots.

Rehd. var. Jiang (Pingyi Tiancha, PYTC) is an excellent apple rootstock and ornamental tree, but its tolerance to salt stress is weak.

[Effects of hydrogen sulfide on root architecture, leaf reactive oxygen and photosynthetic characteristics of Malus hupehensis under waterlogging.].

The root architecture, leaf reactive oxygen species and photosynthetic characteristics of potted Malus hupehensis seedlings were investigated after 10 days of waterlogging under five concentrations of hydrogen sulfide (H2S) donor sodium hydrosulfide (NaSH, 0, 0.02, 0.05, 0.

Inhibition of CDH17 gene expression via RNA interference reduces proliferation and apoptosis of human MKN28 gastric cancer cells.

Gastric cancer is the fourth most common type of cancer and the second cause of cancer‑related mortalities worldwide despite the use of multimodal therapy. Cadherins are transmembrane glycoproteins that are involved in tumorigenesis. CDH17 has been found to be over‑expressed in gastric cancer and its overexpression was associated with lymph node metastasis and tumor‑node‑metastasis stage of the patients, yet the exact role and molecular mechanism of CDH17 in gastric cancer have not been determined.

[Effects of different mulching materials on nitrate metabolism in soil of apple root-zone in summer and autumn.].

This study explored the effects of mulching straw mat, agricultural carpet, transparent-plastic film and horticultural fabric on nitrification-denitrification, nitrate reductase (NR), nitrite reductase (NiR), ammonium, nitrate and nitrite nitrogen in root-zone soil grown with three-year old apple trees (Malus domestica cv. Starkrimson) during summer and autumn. Results showed that the four treatments decreased nitrification intensity in summer soil, NiR activity in summer-autumn soil and the variation coefficient of nitrification-denitrification intensity and NR in both summer and autumn soil.

MhNCED3 in Malus hupehensis Rehd. induces NO generation under osmotic stress by regulating ABA accumulation.

Abscisic acid (ABA) biosynthesis has been widely characterized in plants, whereas the effects of ABA biosynthesis on nitric oxide (NO) generation in osmotic stress are less well understood. In this study, Malus hupehensis Rehd. 9-cis-epoxycarotenoid dioxygenase gene (MhNCED3) which is the key gene in ABA biosynthesis was transformed into wild type (WT) and 129B08/nced3 mutant (AtNCED3 deficient), respectively, and two transgenic Arabidopsis lines were obtained.

A new cholangiojejunostomy for multiple biliary ductal openings: A study in pigs.

Background: To investigate the safety and feasibility of a new intrahepatic cholangiojejunostomy for multiple intrahepatic biliary ductal openings after hepatobiliary resection.

Materials And Methods: Forty-eight pigs were randomly assigned into two groups: the new intrahepatic cholangiojejunostomy group (n = 24); and the control group (n = 24) with classical hepaticojejunostomy. Six pigs in each group were sacrificed on postoperative day 7, 30, 90 and 180.

MhNCED3, a gene encoding 9-cis-epoxycarotenoid dioxygenase in Malus hupehensis Rehd., enhances plant tolerance to Cl- stress by reducing Cl- accumulation.

High Cl(-) concentrations in tissues can be toxic to crop plants and may lead to reduced growth rates and yields. 9-cis-epoxycarotenoid dioxygenase (NCED) is thought to be involved in the biosynthesis of abscisic acid (ABA), which is an important regulator of plant adaptive responses to stress. Here, the expression of MhNCED3 in Malus hupehensis Rehd.

Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells.

Background: Activin A, an important member of transforming growth factor-β superfamily, is reported to inhibit proliferation of mature hepatocyte. However, the effect of activin A on growth of hepatic progenitor cells is not fully understood. To that end, we attempted to evaluate the potential role of activin A in the regulation of hepatic progenitor cell proliferation.

Using protein granularity to extract the protein sequence features.

The feature extraction of protein sequences is a challenging problem. It might need a lot of theoretical and practical knowledge from many fields. The difficulty would increase when investigators extract the features solely from protein sequences.

[Expression of tumor necrosis factor-alpha and caspase-3 protein in monocytes adjacent to the invaded Echinococcus multilocularis in liver].

Objective: To investigate the expression and its significance of tumor necrosis factor-alpha (TNF-alpha) and caspase-3 protein in monocytes adjacent to the invaded Echinococcus multilocularis in liver.

Methods: 40 female Kunming mice were randomly divided into experimental group (n=20) and sham operation (control) group (n=20). Mice in experimental group were infected with 20% E.

HSCs play a distinct role in different phases of oval cell-mediated liver regeneration.

Hepatic stem cell niche plays an important role in hepatic oval cell-mediated liver regeneration. As a component of hepatic stem cell niche, the role of hepatic stellate cells (HSCs) in oval cell proliferation needs further studies. In the present study, we isolated HSCs from rats at indicated time point after partial hepatectomy (PH) in 2-acetylaminofluorene/PH oval cell proliferation model.

[Inhibition of invasive growth and metastasis of hepatic alveolar echinococcosis by anti-osteopontin antibody].

Objective: To observe the inhibitive effect on invasive growth and metastasis of Echinococcus multilocularis by exogenous anti-osteopontin (OPN) antibody.

Methods: 180 gerbils were infected with 20% E. multilocularis suspension (approximately 400 protoscoleces in 0.

[The correlation between osteopontin and metastasis of hepatic Echinococcus multilocularis infection].

Objective: To investigate the expression and distribution of osteopontin (OPN) in Echinococcus multilocularis cyst, and explore the role of OPN in the metastasis of hepatic E. multilocularis infection.

Methods: Forty gerbils were infected with 20% E.

[Endogenous NO and ROS generation in grape roots and leaves under CdCl2 stress].

Taking the cutting seedlings of grape variety 'Ze-xiang' as test materials, a hydroponic experiment was conducted to study the generation patterns of endogenous nitric oxide (NO) and reactive oxygen species (ROS) in their roots and leaves, and the changes of root MDA content and root activity under the stress of cadmium chloride (CdCl2). In the treatments 0-1 mmol x L(-1) of CdCl2, the NO content and NOS activity in roots and leaves and the root activity all presented a tendency of increasing first and decreasing afterwards with increasing CdCl2. At 0.

[Nitric oxide production in Malus hupehensis roots under waterlogging and the effects of exogenous NaNO3 on this production].

Taking two-year old Malus hupehensis as test material, this paper studied its root nitric oxide (NO) production under waterlogging, and the effects of exogenous NaNO3 on this production. Waterlogging for 3-9 days promoted the NO production significantly. Within the 12 days of waterlogging, the NO production, nitrite reductase (NR) and nitric oxide synthase (NOS) activities, and malondialchehyche (MDA) content in roots increased first, and decreased subsequently.

[Effects of cadmium stress on fatty acid composition and lipid peroxidation of Malus hupehensis].

This paper studied the fatty acid composition, reactive oxygen species (ROS), lipoxygenase (LOX) activity, and malondialdehyde (MDA) content in the leaves and roots of Malus hupehensis seedlings under effects of cadmium (Cd) stress. Noticeable changes were observed in the kinds and relative contents of fatty acids after treated with CdCl2 for 7-12 hours. The relative contents of unsaturated fatty acids in leaves and roots reached the maximum after treated for 7 hours, being 82.

[Effects of CdCl2 on grape root mitochondrial characteristics and root activity].

Taking the one-year cutting-seedlings of four grape cultivars (Kyoho, Muscat Hamburg, Long Yan, and Ze Xiang) as test materials, their root mitochondrial hydrogen peroxide (H2O2) content, membrane permeability transition pore (MPTP), membrane potential (Deltapsi), cytochrome C (Cyt c) content, and root activity were measured under effects of CdCl2. For all test grape cultivars, the root mitochondrial H2O2 content and MPTP increased, but the Deltapsi, Cyt c content, and root activity decreased after treated with 0.5 mmol CdCl2 x L(-1).

[Effect of the liposome albendazole and Huai-Er fungus extract on hepatic infection of Echinococcus granulosus in mice].

Objective: To explore the effect of Huai-Er fungus extract (HEF) and liposome albendazole (L-ABZ) on the hepatic infection of Echinococcus granulosus (Eg) in mice, and understand the influence of improved host immunity on postoperative recurrence.

Methods: Female Kunming mice were immune by intraperitoneal injection Eg cyst fluid from sheep, IgG positive mice were divided into drug treatment groups (A, B and C) and control group (D). Mice in group A were administered by gavage with single L-ABZ (75 mg/kg), group B with single HEF (15,000 mg/kg), and group C with combination therapy (L-ABZ 75 mg/kg plus HEF 15,000 mg/kg), once every two days for one week.

[Observation on the ultrastructure of liver cells around hydatid cyst].

Liver tissues around hydatid from 8 patients with cystic echinococcosis were observed by transmission electron microscopy, normal hepatic tissues of 6 cases were used as control. The results demonstrated predominant atrophy and necrosis of hepatocytes. These changes seem to be the major hepatic lesion in cystic echinococcosis.

[Physiological function of arginine and its metabolites in plants].

L-arginine is an important and unique amino acid in plants. It serves not only as an important nitrogen reserve and recycling, but also as a precursor of the biosynthesis of polyamines, nitric oxide and so on. Polyamines and nitric oxide are important messengers involved in almost all physiological and biochemical processes, growth & development, and adaptation of plants to stress.

[Analysis on the anatomic features of 47 cases of hepatic hydatidosis complicated with biliary fistula].

Objective: To propose a criterion and its significance of clinical classification of hepatic hydatidosis complicated with biliary fistula.

Methods: 47 hepatic hydatidosis with biliary fistula cases who were given a subadventitial pericystectomy were observed from 2000 to 2005 in a retrospective study. The methods included observation of the different anatomic features of hepatic hydatidosis complicated with biliary fistula during the surgical operation and evaluation of the curative effect.

[The effect of exogenous H2O2 on mitochondrial membrane permeability and cell nuclear DNA in roots of Malus hupehensis].

To investigate the changes in mitochondrial permeability transition, DNA degradation and cell death, seedling roots of Malus hupehensis Rehd. were treated directly with exogenous H(2)O(2). The results showed that mitochondrial permeability increased obviously by 0.