Study on Calculation Method of Bending Performance of Concrete Sandwich Composite Slab.

In order to explore the flexural behavior of a concrete sandwich panel under concentrated boundary conditions, based on Kirachhoff's elastic thin plate theory in this paper, the geometric deformation, physical conditions, and equilibrium relationship of a sandwich panel are deduced by constructing the layered analysis model of the sandwich panel, the basic differential equation of the flexural deformation of the concrete sandwich thin plate is obtained, and the mathematical expression of the internal force and displacement under the boundary condition of concentrated support is given. Combined with an engineering example, the proposed calculation method is verified. The results show that, in the arrangement of reliable connectors for concrete sandwich panels, the concrete wythes bear the load while the contribution of the core layer to the bending capacity of the structure can be ignored.

Chromosome-level genome assembly and resequencing of camphor tree () provides insight into phylogeny and diversification of terpenoid and triglyceride biosynthesis of .

species attract attentions owing to their scents, medicinal properties, and ambiguous relationship in the phylogenetic tree. Here, we report a high-quality genome assembly of , based on which two whole-genome duplication (WGD) events were detected in the genome: one was shared with Magnoliales, and the other was unique to Lauraceae. Phylogenetic analyses illustrated that Lauraceae species formed a compact sister clade to the eudicots.

Dihydro-Resveratrol Ameliorates Lung Injury in Rats with Cerulein-Induced Acute Pancreatitis.

Acute pancreatitis is an inflammatory process originated in the pancreas; however, it often leads to systemic complications that affect distant organs. Acute respiratory distress syndrome is indeed the predominant cause of death in patients with severe acute pancreatitis. In this study, we aimed to delineate the ameliorative effect of dihydro-resveratrol, a prominent analog of trans-resveratrol, against acute pancreatitis-associated lung injury and the underlying molecular actions.

Immobilization of sp. DG17 onto sodium alginate-attapulgite-calcium carbonate.

A strain of sp. DG17, capable of degrading crude oil, was immobilized in sodium alginate-attapulgite-calcium carbonate for biodegradation of crude oil contaminated soil. In this work, proportion of independent variables, the laboratory immobilization parameters, the micromorphology and internal structure of the immobilized granule, as well as the crude oil biodegradation by sodium alginate-attapulgite-calcium carbonate immobilized cells and sodium alginate-attapulgite immobilized cells were studied to build the optimal immobilization carrier and granule-forming method.

An aptamer-based signal-on bio-assay for sensitive and selective detection of Kanamycin A by using gold nanoparticles.

In this study, a simple and sensitive aptamer-based fluorescence method for the detection of Kanamycin A by using gold nanoparticles (AuNPs) has been developed. In this assay, AuNPs were utilized as DNA nanocarrier as well as efficient fluorescence quencher. In the absence of Kanamycin A, dye-labeled aptamer could be adsorbed onto the surface of AuNPs and the fluorescence signal was quenched.

Pseudosolubilized n-alkanes analysis and optimization of biosurfactants production by Pseudomonas sp. DG17.

The pseudosolubilized medium-chain-length n-alkanes during biodegradation process, and optimization of medium composition and culture conditions for rhamnolipid production by Pseudomonas sp. DG17 using Plackett-Burman design and Box-Behnken design, were examined in this study. The results showed that pseudosolubilized concentration of C14 to C20 n-alkanes was higher than that of C24 to C26.

Factors influencing the trans-membrane transport of -octadecane by sp. DG17.

In soil bioremediation techniques, the trans-membrane transport of hydrocarbons across the cell membrane is a new and complex point of understanding the process of hydrocarbons biodegradation. In this study, the effect of different environmental factors, including substrate concentration, bacterial inoculums, pH, salinity, substrate analogues and nutrients, on the transport of [C]-octadecane by sp. DG17 was investigated.

Uptake and trans-membrane transport of petroleum hydrocarbons by microorganisms.

Petroleum-based products are a primary energy source in the industry and daily life. During the exploration, processing, transport and storage of petroleum and petroleum products, water or soil pollution occurs regularly. Biodegradation of the hydrocarbon pollutants by indigenous microorganisms is one of the primary mechanisms of removal of petroleum compounds from the environment.

Trans-membrane transport of n-octadecane by Pseudomonas sp. DG17.

The trans-membrane transport of hydrocarbons is an important and complex aspect of the process of biodegradation of hydrocarbons by microorganisms. The mechanism of transport of (14)C n-octadecane by Pseudomonas sp. DG17, an alkane-degrading bacterium, was studied by the addition of ATP inhibitors and different substrate concentrations.

[Retrospect of the history of recognition on the mechanism of heart failure and its research in the 19(th) century].

There were the records of heart failure in the 6(th) century B.C. At that time, clinical observation was the main approach.

[Historical review of heart transplantation].

In 1905, Carrel and Guthrie reported the heterotopic heart transplantation on dogs for the first time. In the same year, Shone advanced the transplantation immunity theory which provided a basis for organ transplantation. In 1964, Hardy and his colleagues performed the first human chimpanzee heart transplantation.

Label-free genotyping of cytochrome P450 2D6*10 using ligation-mediated strand displacement amplification with DNAzyme-based chemiluminescence detection.

Genotyping of cytochrome P450 monooxygenase 2D6*10 (CYP2D6*10) plays an important role in pharmacogenomics, especially in clinical drug therapy of Asian populations. This work reported a novel label-free technique for genotyping of CYP2D6*10 based on ligation-mediated strand displacement amplification (SDA) with DNAzyme-based chemiluminescence detection. Discrimination of single-base mismatch is firstly accomplished using DNA ligase to generate a ligation product.

Fluorescence protection assay: a novel homogeneous assay platform toward development of aptamer sensors for protein detection.

Development of novel aptamer sensor strategies for rapid and selective assays of protein biomarkers plays crucial roles in proteomics and clinical diagnostics. Herein, we have developed a novel aptamer sensor strategy for homogeneous detection of protein targets based on fluorescence protection assay. This strategy is based on our reasoning that interaction of aptamer with its protein target may dramatically increase steric hindrance, which protects the fluorophore, fluorescein isothiocyannate (FITC), labeled at the binding pocket from accessing and quenching by the FITC antibody.

[Effects of combined pollution of lead and benzo[a] pyrene on seed growth of wheat in soils].

Seed germination, root elongation, shoot elongation and ratio of shoot to root of wheat in soils polluted by lead (Pb) and benzo (a)pyrene (B[a] P) with medium-low concentrations were studied to reveal the ecological effects of combined pollution and screen the indicative markers. Results indicated that seed germination was not sensitive to single or combined pollution of Pb or B[a] P. Root elongation was inhibited by single pollution of Pb or B[a]P to different extents.

Homogeneous label-free genotyping of single nucleotide polymorphism using ligation-mediated strand displacement amplification with DNAzyme-based chemiluminescence detection.

Genotyping of single nucleotide polymorphisms (SNPs) is a central challenge in disease diagnostics and personalized medicine. A novel label-free homogeneous SNP genotyping technique is developed on the basis of ligation-mediated strand displacement amplification (SDA) with DNAzyme-based chemiluminescence detection. Discrimination of single-base mismatches is first accomplished using DNA ligase to generate a ligation product between a discriminant probe and a common probe.

Fluorescence aptameric sensor for strand displacement amplification detection of cocaine.

A new fluorescence method based on aptamer-target interactions has been developed for cocaine detection with target-induced strand displacement. Here we describe new probes, the hairpin-probe and the single strand-probe (ss-probe), that possess two recognition sequences of cocaine aptamer. In the presence of cocaine, both probes would associate with the target to form a tripartite complex.

[Correlation of heat shock protein 70 expression in nasopharyngeal carcinoma to immunoglobin A against viral capsid antigen of Epstein-Barr virus in sera and its clinical significance].

Background & Objective: Overexpression of heat shock protein (HSP)70 is expressed in many tumors, but the correlation of its overexpression in nasopharyngeal carcinoma (NPC) to immunoglobin A against viral capsid antigen of Epstein-Barr virus (EBV) in sera and its clinical significance are still unclear. This study was to determine the expression of HSP70 in NPC, and to analyze its correlations to EBV IgA/VCA titer and prognosis.

Methods: The expression of HSP70 in 38 specimens of stage II-III NPC was determined by SP immunohistochemistry; the content of HSP70 in the 38 specimens was detected by ELISA; the EBV IgA/VCA titer in sera of the 38 patients was detected by immunoenzymatic (IE) method.

EDTA-enhanced phytoremediation of lead contaminated soil by Bidens maximowicziana.

Phytoremediation is a potential cleanup technology for the removal of heavy metals from contaminated soils. Bidens maximowicziana is a new Pb hyperaccumulator, which not only has remarkable tolerance to Pb but also extraordinary accumulation capacity for Pb. The maximum Pb concentration was 1509.

[Effects of rhamnolipid on the biodegradation of n-hexadecane by microorganism and the cell surface hydrophobicity].

The experiments were made in laboratory to analyze the effects of rhamnolipid on the biodegradation of n-hexadecane and the cell surface hydrophobicity using the microorganism ( Bacillus cereus DQ01 and Bacillus sp. DQ02). The results show that the optimal concentrations range of rhamnolipid were 0.

Isolation and characterization of gasoline-degrading bacteria from gas station leaking-contaminated soils.

The effects of culture conditions in vitro and biosurfactant detection were studied on bacterial strains capable of degrading gasoline from contaminated soils near gas station. The main results were summarized as follows. Three bacteria (strains Q 10, Q14 and Q18) that were considered as efficiently degrading strains were isolated and identified as Pseudomonas sp.

[Bidens maximowicziana's adsorption ability and remediation potential to lead in soils].

Bidens maximowicziana's adsorption ability and remediation potential to lead were studied. The results show: (1) The Bidens maximowicziana has a strong adsorption to lead, the concentration of lead in plants increased linearly with the increase of lead concentration in soil. Then maximum concentration was 1509.