Clinical Application of Double Ovulation Stimulation in Patients with Diminished Ovarian Reserve and Asynchronous Follicular Development Undergoing Assisted Reproduction Technology.

Objective: This study aimed to compare the clinical effects of double ovulation stimulation (DouStim) applied during the follicular and luteal phases with the antagonist protocol in patients with diminished ovarian reserve (DOR) and asynchronous follicular development undergoing assisted reproductive technology (ART).

Methods: The clinical data of patients with DOR and asynchronous follicular development receiving ART from January 2020 to December 2021 were retrospectively analyzed. The patients were divided into two groups according to their ovulation stimulation protocol: DouStim group (n=30) and antagonist group (n=62).

Distribution and identification of Mycobacterium tuberculosis lineage in Kashgar prefecture.

Objectives: Kashgar prefecture is an important transportation and trade hub with a high incidence of tuberculosis. The following study analyzed the composition and differences in Mycobacterium tuberculosis (M.tb) lineage and specific tags to distinguish the lineage of the M.

[Delayed decompression for cauda equina syndrome secondary to lumbar disc herniation: long-term follow-up results].

Objective: To assess the impact of delayed decompression on long-term neurological and bladder function recovery in patients with cauda equina syndrome (CES) secondary to lumbar disc herniation (LDH).

Methods: The clinical data of 35 patients receiving delayed decompression surgery for CES secondary to LDH were reviewed. The bladder empty function, bowel control, sexual ability and neurological functions of the lower limbs were evaluated after the operation, and the urodynamic changes were assessed in 6 patients with urodynamic data before and after the operation.

Reproductive management through integration of PGD and MPS-based noninvasive prenatal screening/diagnosis for a family with GJB2-associated hearing impairment.

A couple with a proband child of GJB2 (encoding the gap junction protein connexin 26)-associated hearing impairment and a previous pregnancy miscarriage sought for a reproductive solution to bear a healthy child. Our study aimed to develop a customized preconception-to-neonate care trajectory to fulfill this clinical demand by integrating preimplantation genetic diagnosis (PGD), noninvasive prenatal testing (NIPT), and noninvasive prenatal diagnosis (NIPD) into the strategy. Auditory and genetic diagnosis of the proband child was carried out to identify the disease causative mutations.

Human amniotic fluid-derived stem cells can differentiate into hepatocyte-like cells in vitro and in vivo.

Although human amniotic fluid is an attractive source of multipotent stem cells, the potential of amniotic fluid stem cells (AFSCs) to differentiate into hepatic cells has not been extensively evaluated. In this study, we examined whether human AFSCs can differentiate into a hepatic cell lineage in vitro and in vivo. After being treated with cytokines (fibroblast growth factor 4, basic fibroblast growth factor, hepatocyte growth factor, and oncostatin), AFSCs developed a morphology similar to that of hepatocytes.

[Predictive value of fluorescence in situ hybridization in patients with bladder cancer].

Objective: To assess the value of fluorescence in situ hybridization (FISH) in the diagnosis of bladder cancer.

Methods: Urine samples from 100 patients suspected of having bladder cancer were collected before cystoscopy for immediate urine cytology and FISH analysis. The criteria for FISH abnormality were determined by evaluating the urine specimens from 20 subjects without urogenital neoplasm.

Synthesis, structures, and solution dynamics of palladium complexes of quinoline-functionalized N-heterocyclic carbenes.

A new type of quinoline-functionalized palladium N-heterocyclic carbene (NHC) complexes has been synthesized via silver transmetallation. The quinoline moiety was either directly attached to the imidazole ring or linked to it by a methylene group. NHCs with a methylene linker tend to form trans biscarbene complexes in the reaction of Pd(COD)Cl2, while NHCs without any linker form chelating NHC-quinoline (NHC-N) complexes.

Long-lived radical cations as model compounds for the reactive one-electron oxidation product of vitamin E.

Heterocyclic compounds with structures similar to vitamin E, but without the hydroxyl hydrogen atom, were synthesized and their electrochemical behavior examined in acetonitrile solutions and as solids in aqueous solutions of varying pH by attaching the compounds to the surface of a glassy carbon electrode. Compound 1, containing a fully methylated aromatic ring was found to be the most long-lived following one-electron oxidation, with its radical cation (1+*) surviving in acidic aqueous solutions and able to be isolated as a salt, 1+*(SbF6-), when reacted with NOSbF6 in CH3CN. Electrochemical, UV-vis and FTIR experiments on 1+*, in addition to the results from theoretical calculations, indicated that the electrochemical, electronic and structural properties of 1+* are very similar to those of the radical cation of vitamin E.

Variable scan rate cyclic voltammetry and theoretical studies on tocopherol (vitamin E) model compounds.

Variable scan rate (0.1-500 V s(-1)) cyclic voltammetry experiments were performed on a series of model tocopherol (vitamin E) compounds with differing degrees of methyl substitution around the aromatic (phenolic) ring. alpha-Tocopherol, with a fully methylated aromatic ring, produced stable phenoxonium cations upon oxidation in CH3CN, and was modeled via an ECE mechanism (where "E" represents an electron transfer and "C" a chemical step).

Investigation into phenoxonium cations produced during the electrochemical oxidation of chroman-6-ol and dihydrobenzofuran-5-ol substituted compounds.

A series of chroman-6-ol and dihydrobenzofuran-5-ol based compounds with structures similar to vitamin E were examined by cyclic voltammetry and controlled potential electrolysis. The compounds displayed characteristic voltammetric features that enabled their electrochemical behavior to be interpreted in relation to the oxidation mechanism for vitamin E. The electrochemical experiments indicated the presence of several oxidized species: cation radicals, phenoxyl radicals, phenoxonium ions, hemiketals, and p-quinones, whose lifetimes varied depending on the extent of methylation of the aromatic ring (R(1), R(2), R(3)) and the nature of substituents R(4) and R(5).

[Establishment and characteristics of clonal human embryonic stem cell lines].

Objective: To establish clonal human embryonic stem cell lines and investigate their biological characteristics.

Methods: Cells were derived from one inner cell mass of human blastocyst, multiplied for 20 passages, and then dissociated into single cell suspension by digestion with 0.5% trypsin.

Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines.

Background: Serum-containing and serum-free media were used to derive human embryonic stem (HES) cells from donated oocytes and embryos.

Methods And Results: Inner cell masses (ICM) were isolated by immunosurgery. The HES cells were found to be easily obtained and expanded in a serum-free medium.

[Human embryonic stem cells forming embryoid bodies comprising multiple types of cells].

Objective: To investigate the pluripotency of human embryonic stem cells in vitro.

Methods: Human embryonic stem cells were cultured in suspension without feeder layers and bFGF in medium. mRNAs of different types of cells were analyzed by RT-PCR.

[Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes].

Objective: To investigate the influence of cryoprotectants and cooling rates in vitrification method on the spindles of rabbit M II oocytes.

Methods: Rabbit oocytes were verified by using cryoloop with ethylene glycol (EG) singly or EG combined with dimethyl sulphoxide (DMSO) as cryoprotectants, and cooled by taking oocytes directly into liquid nitrogen or by vitrification machine. After frozen rabbit oocytes thawed, the microtubulin and chromosome of the spindles were fixation and stained by immunofluorescent method.

[Establishment of a single cell-cloned mouse embryonic stem cell line].

Objective: To establish embryonic stem cells culture system and methods.

Methods: Inner cell masses were isolated from blastocysts of 3.5 day-old 129SvJ mice by immunosurgery, seeded onto gamma-irradiated mouse fibroblasts feeder layer.