sp. nov., isolated from Darangshi Oreum (a volcanic cone), and the reclassification of Lu . 2003 as a later heterotypic synonym of (Shinobu 1962) Preobrazhenskaya . 1975 (Approved Lists 1980).

Two mycelium-forming actinobacterial strains, designated as DLS-47 and DLS-62, were isolated from volcanic ash collected from the surface of a rock on the peak of Darangshi Oreum (a volcanic cone) in Jeju, Republic of Korea, and their taxonomic positions were investigated by a polyphasic approach. Both of the isolates showed growth at 20-42 °C, pH 6.0-9.

sp. nov., sp. nov., and sp. nov., isolated from dried beach seaweeds.

Three actinobacterial strains, KSW2-21, KSW2-29 and KSW4-17, were isolated from dried seaweeds collected around Gwakji Beach in Jeju, Republic of Korea. Their taxonomic positions were determined based on genomic, physiological and morphological characteristics. The isolates were Gram-positive, aerobic, non-motile, rod-shaped bacteria characterized by the following chemotaxonomic features: ornithine as the cell wall diamino acid, the -glycolyl type of murein, MK-11 as the predominant menaquinone, polar lipids including diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and four unidentified phospholipids, with anteiso-C, iso-C and anteiso-C as the the major fatty acids.

Microbacterium aquilitoris sp. nov. and Microbacterium gwkjiense sp. nov., isolated from beach.

Three Gram-reaction-positive bacterial strains, designated KSW-18, KSW2-22, and KSW4-11, were isolated from seawater, and two dried seaweed samples collected at Gwakji Beach in Jeju, Republic of Korea, respectively, and their taxonomic positions were examined by a polyphasic approach. The 16S rRNA gene phylogeny showed that strain KSW4-11 was tightly associated with Microbacterium oleivorans NBRC 103075, while strains KSW-18 and KSW2-22 formed a distinctive subline at the base of a clade including the above two strains. The three isolates showed high sequence similarity with one another (99.

gen. nov. sp. nov., a novel bacterium of the order isolated from a cave and a proposal of fam. nov.

Two Gram-stain-positive, aerobic, non-spore-forming, non-motile, irregular rod-shaped actinobacteria, designated as D2-41 and D3-21, were isolated from soil samples collected in a natural cave in Jeju, Republic of Korea. Both of the isolates were shown to share 100 % 16S rRNA sequence identity. The cell wall contained -diaminopimelic acid, arabinose and galactose.

Four new species isolated from seaweeds and reclassification of five species with a proposal of gen. nov. under a genome-based framework of the genus .

The taxonomic relationships of 10 strains isolated from seaweeds collected from two beaches in Republic of Korea were studied by sequencing and analyses of 16S rRNA genes and whole genomes. For the construction of a more reliable and robust 16S rRNA gene phylogeny, the authentic and nearly complete 16S rRNA gene sequences of all the type strains were selected through pairwise comparison of the sequences contained in several public databases including the List of Prokaryotic names with Standing in Nomenclature (LPSN). The clustering of the ten study strains into five distinct groups was apparent in this single gene-based phylogenetic tree.

sp. nov. and OS3-89, isolated from rhizosphere soil of cactus.

Two mycelium-forming actinobacterial strains, designated OS3-83 and OS3-89, were isolated from rhizosphere soil of a cactus () sampled on Mara Island, Jeju, Republic of Korea. Both of the isolates were found to grow at 20-37 °C, pH 6.0-10.

sp. nov., isolated from a lava cave.

A Gram-reaction-positive, strictly aerobic, non-sporulating, non-motile, rod-shaped bacterium, designated YC3-14, was isolated from pieces of stalagmite collected in a lava cave in Jeju, Republic of Korea. Cells showed growth at 15-35 °C, pH 6.0-9.

sp. nov. and sp. nov., isolated from larvae of .

The taxonomic positions of two novel strains isolated from larvae of an insect () collected in Jeju, Republic of Korea, were determined by a polyphasic approach. Strain BWB3-3 was closely related to the type strain of , having 97.2 % 16S rRNA gene sequence similarity, whereas strain BWM-S5 formed an independent cluster within the genus in the 16S rRNA gene phylogeny and the closest relative was the type strain of (98.

gen. nov., sp. nov., isolated from larvae of mealworm L., and a proposal to transfer Liu . 2016 to a new genus as comb. nov.

Two enterobacterial strains, designated YMB-R21 and YMB-R22, were isolated from larvae of mealworm L. and examined for their taxonomic characteristics. A 16S rRNA gene-based neighbour-joining tree showed that the two isolates formed two distinct sublineages within the family and were separated from other genera of the family.

sp. nov., isolated from larvae of , proposal of gen. nov., comb. nov. and emended descriptions of the genera , and .

Taxonomic positions of four Gram-negative bacterial strains, which were isolated from larvae of two insects in Jeju, Republic of Korea, were determined by a polyphasic approach. Strains CWB-B4, CWB-B41 and CWB-B43 were recovered from larvae of , whereas strain BWR-B9 was from larvae of . All the isolates grew at 10-37 °C, at pH 5.

Rouxiella aceris sp. nov., isolated from tree sap and the emended description of the genus Rouxiella.

Polyphasic taxonomic studies were performed for the seven strains, which were isolated from sap extracted from Acer pictum in Mt. Halla in Jeju, Republic of Korea. Cells of all the isolates were Gram-reaction-negative, facultatively anaerobic, short rods and contained the major isoprenoid quinone of Q-8, the predominant fatty acids of C and C cyclo and the major polar lipids including phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid.

Hongsoonwoonella zoysiae gen. nov., sp. nov., a new member of the family Stappiaceae isolated from a tidal mudflat.

A Gram stain-negative bacterial strain, designated SY4-7, was isolated from rhizosphere mudflat of a halophyte (Zoysia sinica) collected around Seonyu Island, Republic of Korea. Cells of the organism were strictly aerobic, non-sporulating, non-motile rods and grew at 20-42 °C, pH 6-8 and 1-6% (w/v) NaCl. The 16S rRNA gene-based phylogenetic analyses revealed that strain SY4-7 formed an independent cluster separated from the recognized genera of the family Stappiaceae, which was also supported by phylogenomic analysis-based 92-core gene sequences.

Nitratireductor kimnyeongensis sp. nov., isolated from seaweed.

An aerobic, Gram-negative, catalase-positive, oxidase-positive bacterium was isolated from a dried seaweed sample collected from Kimnyeong Beach in Jeju, Republic of Korea. The cells of the organism, designated strain KY 101(T), were rods (0.4-0.