Outcome after pelvic sarcoma resection and reconstruction with a modular hemipelvic prostheses.

Purpose: The aim of this study was to evaluate the early clinical outcome of reconstruction with modular hemipelvic prostheses after pelvic sarcoma resection.

Methods: We retrospectively reviewed eight patients between 2004 and 2007 who had periacetabular resections and reconstruction with a modular hemipelvic prosthetic system for pelvic sarcoma with a mean follow-up of 27 (range,10~54) months. Oncology outcome was assessed with survival rate, local recurrence rate, International Society of Limb Salvage (ISOLS) score and complications.

Evaluation of bioabsorbable multiamino acid copolymer/α-tri-calcium phosphate interbody fusion cages in a goat model.

Study Design: A study of cervical interbody fusion using polyamino acid-based bioabsorbable fusion cages in a goat model.

Objective: To compare interbody fusion of a bioabsorbable multiamino acid copolymer/α-tri-calcium phosphate (MAACP/α-TCP) fusion cage with an autologous tricortical iliac-crest bone graft and a titanium cage.

Summary Of Background Data: Polyamino acid is widely used as a carrier for drug delivery.

Preliminary effect of proximal femoral nail antirotation on emergency treatment of senile patients with intertrochanteric fracture.

Objective: To retrospectively analyse the clinical outcome of emergency treatment of senile intertrochanteric fractures with proximal femoral nail antirotation (PFNA).

Methods: From September 2008 to March 2009, 35 senile patients with intertrochanteric fracture, aged from 65 to 92 years with an average age of 76.5 years, were treated with PFNA within 24 hours after injury.

Mesojugular shunt for Budd-Chiari syndrome: a case report with long-term follow-up.

Background: We report a case of a mesojugular shunt for Budd-Chiari syndrome (BCS) with long-term follow-up.

Methods: A 32-year-old woman presented with late stage BCS. She was treated with a mesojugular shunt to relieve her portal hypertension.

Volumetric topological analysis: a novel approach for trabecular bone classification on the continuum between plates and rods.

Trabecular bone (TB) is a complex quasi-random network of interconnected plates and rods. TB constantly remodels to adapt to the stresses to which it is subjected (Wolff's Law). In osteoporosis, this dynamic equilibrium between bone formation and resorption is perturbed, leading to bone loss and structural deterioration.

Immunosuppressive sesquiterpenes from Buddleja daviddi.

Six new sesquiterpenes, 2,6(12),10-humulatrien-7β-ol-1-one (1), 2 α-acetoxy-5α-methoxy-enantio-caryophylla-8(15)-en-3-one (2), 2α-acetoxy-5α-hydroxy-enantio-caryophylla-8(15)-en-3-one (3), 2α-acetoxy-4β,5α-hydroxy-enantio-caryophylla-8(15)-en-3-one ( 4), 2α-acetoxy-4β,5β-hydroxy-enantio-caryophylla-8(15)-en-3-one (5), 2β-acetoxy-4-caryophyllen-8β-ol-3-one (6), and nineteen known compounds were isolated from the ethanol extract of Buddleja daviddi. The structures were elucidated by spectroscopic methods. Compounds 8-11, 14, 16, 17, and 20 showed significant immunosuppressive activities, and 8-11 and 14 were cytotoxic on HeLa and L929 cell lines.

[Three-dimensional finite element analysis and biomechanical study on reconstruction of the large defect of proximal femur with allograft prosthesis composite in clinical bone-healing phase].

Three-dimensional finite element models of the large defect of proximal femur were reconstructed with allograft prosthesis composite in clinical bone-healing phase; current model was under the given conditions of 138mm-intramedullary stem-length of host bone and 135mm defect-length of proximal femur. The femur was constructed with efilm software from CT data, then three-dimensional concrete models were created by using Proe-Wildfire software; the three-dimensional finite element models of allograft prosthesis composite were made in ANSYS11 software. Loads were simulated using the peaking values during stance walking.

Valgus osteotomy combined with intramedullary nail for Shepherd's crook deformity in fibrous dysplasia: 14 femurs with a minimum of 4 years follow-up.

Background And Objectives: To investigate the effectiveness of valgus osteotomy combined with intramedullary nail in treatment of Shepherd's crook deformity of fibrous dysplasia.

Method: A retrospective study was performed in 13 patients (14 femora) of fibrous dysplasia who were treated in our hospital between August 2000 and February 2005. All patients had Shepherd's crook deformity.

[Early use of vacuum sealing drainage to repair the wound of the injured in Wenchuan earthquake].

Objective: To investigate the method of using vacuum sealing drainage (VSD) technique to repair the wound of the injured in Wenchuan earthquake and its therapeutic effect.

Methods: From May 12, 2008 to June 12, 2008, 52 injured persons (83 wounds) were treated, including 27 males and 25 females aged 11-83 years old (average 42 years old). There were 22 cases of crush injury, 9 cases of contusion and laceration injury, 17 cases of compression injury receiving incision decompression of fascia compartment and 4 cases of open amputation.

[Surgical treatment for fibrous dysplasia of bone involving proximal femur].

Objective: To find an effective method of surgical treatment of fibrous dysplasia of bone involving the proximal femur.

Methods: From January 2001 to January 2006, 57 patients with fibrous dysplasia of bone involving the proximal femur were treated. There were 29 males and 28 females, aging 8-50 years (mean 22 years).

Surgical technique and clinical results for scapular allograft reconstruction following resection of scapular tumors.

Background: Progress in developing effective surgical techniques, such as scapular allograft reconstruction, enhance shoulder stability and extremity function, in patients following scapular tumor resection.

Methods: Case details from seven patients who underwent scapular allograft reconstruction following scapular tumor resection were reviewed. A wide marginal resection (partial scapulectomy) was performed in all patients and all affected soft tissues were resected to achieve a clean surgical margin.

Frequent unanticipated alleles of lethal giant larvae in Drosophila second chromosome stocks.

Forty years ago, a high frequency of lethal giant larvae (lgl) alleles in wild populations of Drosophila melanogaster was reported. This locus has been intensively studied for its roles in epithelial polarity, asymmetric neural divisions, and restriction of tissue proliferation. Here, we identify a high frequency of lgl alleles in the Bloomington second chromosome deficiency kit and the University of California at Los Angeles Bruinfly FRT40A-lethal P collection.

Harmonic wavelet analysis of modulated tunable diode laser absorption spectroscopy signals.

Wavelet analyses of tunable diode laser absorption spectroscopy signals were performed. The absorption spectroscopy data were obtained by repeatedly scanning the beams from a tunable diode laser operating in the near infrared across absorption lines of gaseous NH(3) contained within a windowed glass tube. The laser was modulated and wavelet analyses of the absorption data were performed.

[Congenital bilateral perisylvian syndrome: a case report].

Congenital bilateral perisylvian syndrome (CBPS) is rare in literature, especially in China. In this article, we report the clinical and treatment of a patient with CBPS and discuss its mechanism, clinical features and therapy. This patient was a 28-year-old man.

[Association of single nucleotide polymorphisms of GATA3 with allergic rhinitis phenotypes in Chinese].

Objective: To evaluate the association between single nucleotide polymorphisms (SNP) of GATA3 and Chinese with allergic rhinitis.

Methods: This study included 109 patients with allergic rhinitis and 112 healthy control people. SNP of two loci with restriction fragment length polymorphism was detected, rs1269486 at promoter region and rs2229360 located 92 bp downstream of STOP codon.

Association of single nucleotide polymorphisms in GATA-3 with allergic rhinitis.

Conclusion: The results of our study demonstrated the association between the single nucleotide polymorphisms (SNPs) of GATA-3 and allergic rhinitis (AR).

Objective: AR is considered to be controlled by T-helper type 2 (Th2) cells. GATA-3 is the crucial transcription factor controlling the differentiation and function of Th2 cells.

The regulatory activity of microRNA* species has substantial influence on microRNA and 3' UTR evolution.

During microRNA (miRNA) biogenesis, one strand of a approximately 21-22-nucleotide RNA duplex is preferentially selected for entry into a silencing complex. The other strand, known as the miRNA* species, has typically been assumed to be a carrier strand. Here we show that, although Drosophila melanogaster miRNA* species are less abundant than their partners, they are often present at physiologically relevant levels and can associate with Argonaute proteins.

Biomechanical and histological evaluation of the application of biodegradable poly-L-lactic cushion to the plate internal fixation for bone fracture healing.

Background: Internal plate fixation of fracture can provide favorable mechanical environment for fracture fragments. However, osteoporosis under the plate is often found, and refracture may occur after the plate is removed. There are two different opinions about the bone loss beneath the plate: first is the stress shielding effect brought by the rigid fixation, and the second is the insufficient blood supply of the bone caused by the placement of the plate.

Preparation and in vitro activity of controlled release microspheres incorporating bFGF.

Objective: To study the preparative method of controlled release microspheres incorporating basic fibroblast growth factor (bFGF) and the bioactivities of bFGF, which were released from bFGF microspheres, on the cultured Schwann cells.

Methods: bFGF was microcapsulated with the multiple emulsion encapsulative method using polylactic-co-glycolic acid (PLGA) as coating material. Its morphology, particle size distribution, drug loading, enveloping rate and in vitro release property were studied.

Expression and functional analysis of a novel Fusion Competent Myoblast specific GAL4 driver.

In the Drosophila embryo, body wall muscles are formed by the fusion of two cell types, Founder Cells (FCs) and Fusion Competent Myoblasts (FCMs). Using an enhancer derived from the Dmef2 gene ([C/D]( *)), we report the first GAL4 driver specifically expressed in FCMs. We have determined that this GAL4 driver causes expression in a subset of FCMs and, upon fusion, in developing myotubes from stage 14 onwards.

A key role of Pox meso in somatic myogenesis of Drosophila.

The Pax gene Pox meso (Poxm) was the first and so far only gene whose initial expression was shown to occur specifically in the anlage of the somatic mesoderm, yet its role in somatic myogenesis remained unknown. Here we show that it is one of the crucial genes regulating the development of the larval body wall muscles in Drosophila. It has two distinct functions expressed during different phases of myogenesis.

The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila.

The canonical microRNA (miRNA) pathway converts primary hairpin precursor transcripts into approximately 22 nucleotide regulatory RNAs via consecutive cleavages by two RNase III enzymes, Drosha and Dicer. In this study, we characterize Drosophila small RNAs that derive from short intronic hairpins termed "mirtrons." Their nuclear biogenesis appears to bypass Drosha cleavage, which is essential for miRNA biogenesis.

[Separation and identification of the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and initial investigation of their protein composition].

Objective: To separate and identify the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and to detect their protein composition, and to investigate the possibility of using these exosomes as a kind of tumor vaccine.

Methods: Exosomes were purified by serial ultracentrifugation and sugar density ultracentrifugation, and then they were observed and identified by electron microscopy. Exosomes underwent peptide mass fingerprint and Western blot analyses.