Applicability of intI1 as an indicator gene for securing the removal efficiency of extracellular antimicrobial resistance genes in full-scale wastewater treatment plants.

Mitigating the release of extracellular antimicrobial resistance genes (exARGs) from wastewater treatment plants (WWTPs) is crucial for preventing the spread of antimicrobial resistance from human domains into the environment. This study aimed to evaluate the applicability of intI1 as a performance indicator for securing the removal of exARGs at WWTPs. We investigated the reduction of exARGs and intI1 in a full-scale WWTP, where identical wastewater was treated using conventional activated sludge (CAS) and membrane bioreactor (MBR) systems.

Molecular tag for promoting -glycan maturation in the cargo receptor-mediated secretion pathway.

MCFD2 and ERGIC-53 form a cargo receptor complex that plays a crucial role in transporting specific glycoproteins, including blood coagulation factor VIII, from the endoplasmic reticulum to the Golgi apparatus. We have demonstrated that MCFD2 recognizes a 10-amino-acid sequence in factor VIII, thereby facilitating its efficient transport. Moreover, the secretion of biopharmaceutical recombinant glycoproteins, such as erythropoietin, can be enhanced by tagging them with this sequence, which we have termed the "passport sequence" (PS).

Non-small cell lung cancer with synchronous brain metastases: Identification of prognostic factors in a retrospective multicenter study (HOT 1701).

Background: Non-small-cell lung cancer (NSCLC) is associated with a high incidence of brain metastasis (BM), and the prognosis of patients with NSCLC and BM is poor. This study aimed to identify the prognostic factors and elucidate the survival rates of Japanese patients with NSCLC and BM at initial diagnosis.

Methods: HOT 1701 is a retrospective multicenter study of patients with NSCLC and BM at initial diagnosis.

Fibre post removal using ultrasonic tips: A comparative in vitro study using different protocols.

This study evaluated the glass fibre post (GFP) removal using three different protocols with ultrasonic tips. Thirty-six extracted single-rooted teeth were endodontically treated and cemented with a 10-mm GFP. Three protocols were used for GFP removal: 20 s activated with cooling, 20 s activated without cooling and 40 s activated without cooling.

A case report: pitfalls in antibacterial therapy with rifampicin for mechanical valve endocarditis-the king of drug interactions.

Background: Rifampicin is a strong inducer of the hepatic cytochrome P450 (CYP) family and is known to interact with many clinical drugs. However, to our knowledge, no case of worsening heart failure (HF) due to the interaction between rifampicin and HF drugs has been reported.

Case Summary: A 32-year-old female, who had undergone intracardiac repair for an incomplete atrioventricular septal defect with dextrocardia and prosthetic valve replacements for right and left atrioventricular valve regurgitation, presented as an outpatient.

Fungal toxin fusicoccin enhances plant growth by upregulating 14-3-3 interaction with plasma membrane H-ATPase.

Fusicoccin-A (FC-A) is a diterpene glucoside produced by a pathogenic fungus. Since its discovery, FC-A has been widely recognized as a phytotoxin that induces stomatal opening and leaf wilting, eventually leading to plant death. In this study, we present the first evidence that FC-A enhances plant growth by stabilizing the protein-protein interaction between plasma membrane (PM) H-ATPase and 14-3-3 in guard cells.

Retrospective study on penicillin allergy delabeling and evaluation of an antibiotic allergy assessment tool.

Background: It has been reported that 95 % of patients labeled as penicillin-allergic may be mislabeled. However, the process of delabeling has not advanced due to a shortage of allergy specialists capable of conducting accurate assessments. To address this issue, The Antibiotics Allergy Assessment Tool (AAAT) has been proposed as a tool to evaluate patients who can be delabeled by non-specialists.

Antibiotic resistance genes and crAssphage in hospital wastewater and a canal receiving the treatment effluent.

Hospital wastewater is a major hotspot for the spread of antimicrobial resistance (AMR) in aquatic ecosystems. This study aimed to investigate the prevalence of antibiotic resistance genes (ARGs) and their correlation with crAssphage in a hospital wastewater treatment plant (HWWTP) and a receiving canal. Water samples were analyzed for 94 ARGs and crAssphage relative to the 16S rRNA using high-throughput quantitative polymerase chain reaction (HT-qPCR).

Intrinsic signaling pathways modulate targeted protein degradation.

Targeted protein degradation is a groundbreaking modality in drug discovery; however, the regulatory mechanisms are still not fully understood. Here, we identify cellular signaling pathways that modulate the targeted degradation of the anticancer target BRD4 and related neosubstrates BRD2/3 and CDK9 induced by CRL2- or CRL4 -based PROTACs. The chemicals identified as degradation enhancers include inhibitors of cellular signaling pathways such as poly-ADP ribosylation (PARG inhibitor PDD00017273), unfolded protein response (PERK inhibitor GSK2606414), and protein stabilization (HSP90 inhibitor luminespib).

Development of optimization method for truss structure by quantum annealing.

In this study, we developed a new method of topology optimization for truss structures by quantum annealing. To perform quantum annealing analysis with real variables, representation of real numbers as a sum of random number combinations is employed. The nodal displacement is expressed with binary variables.

Therapeutic effect of dose-dense paclitaxel plus carboplatin with or without bevacizumab for Japanese patients with epithelial ovarian cancer.

Background: Evidence regarding chemosensitivity to different therapeutic regimens in epithelial ovarian cancer (EOC) remains limited. This study aimed to investigate EOC implementation in daily clinical practice and reveal favorable regimens for EOC among Japanese patients.

Methods: We retrospectively collected clinical data of patients newly diagnosed with EOC from 2012 to 2021 at our affiliated institutions.

Optimizing start-up strategies for the two-inflow nitritation/anammox process: Influence on biofilm microbial community composition.

Low-energy nitrogen removal from ammonium-rich wastewater is crucial in preserving the water environment. A one-stage nitritation/anammox process with two inflows treating ammonium-containing wastewater, supplied from inside and outside the wound filter, is expected to stably remove nitrogen. Laboratory-scale reactors were operated using different start-up strategies; the first involved adding nitritation inoculum after anammox biomass formation in the filter, which presented a relatively low nitrogen removal rate (0.

Real-Time On-Site Monitoring of Viruses in Wastewater Using Nanotrap Particles and RICCA Technologies.

Wastewater-based epidemiology (WBE) is an effective and efficient tool for the early detection of infectious disease outbreaks in a community. However, currently available methods are laborious, costly, and time-consuming due to the low concentration of viruses and the presence of matrix chemicals in wastewater that may interfere with molecular analyses. In the present study, we designed a highly sensitive "Quick Poop (wastewater with fecal waste) Sensor" (termed, QPsor) using a joint approach of Nanotrap microbiome particles and RICCA (RNA Isothermal Co-Assisted and Coupled Amplification).

On the Disproportionate Contribution of Membrane Electron Donor Functionality in Membrane Biofouling.

This study set out to uncover which interfacial properties have the greatest impact on membrane organic fouling, biofouling, and fouling resistance. A relatively simple manipulation of the basic equations used in determining Lifshitz-van der Waals (LW) and Lewis acid-base (AB) surface tensions for solid materials reveals that the high electron accepticity of water makes the electron donicity of membrane and biofouling materials the key component governing their interfacial free energy of adhesion (Δ), which defines the favorability (or unfavorability) of one material (1) adhering to another (2) when immersed in a liquid (3). Various biofoulant and membrane LW and AB surface tensions were systematically characterized.

Design Rationale for CO Separation Membranes with Micropatterned Surface Structures.

Here, we report the design rationale of CO separation membranes with micropatterned surface structures. Thin film composite (TFC) membranes with micropatterned surface structures were fabricated by spray coating amine-containing hydrogel particles on the top of micropatterned porous support membranes, which were synthesized by a polymerization-induced phase separation process in a micromold (PIPsμM). The pore size of the support membranes was optimized by tuning the proportion of good and poor solvents for the polymerization process so that the microgels would be assembled as a defect-free separation layer.