A Single Trophoblast Layer Acts as the Gatekeeper at the Endothelial-Hematopoietic Crossroad in the Placenta.

During embryonic development the placental vasculature acts as a major hematopoietic niche, where endothelial to hematopoietic transition ensures emergence of hematopoietic stem cells (HSCs). However, the molecular mechanisms that regulate the placental hematoendothelial niche are poorly understood. Using a parietal trophoblast giant cell (TGC)-specific knockout mouse model and single-cell RNA-sequencing, we show that the paracrine factors secreted by the TGCs are critical in the development of this niche.

3D scanning a crime scene to enhance juror understanding of Bloodstain Pattern Analysis evidence.

There are numerous crime scene investigation applications of 3D scanning that have been previously documented. This paper documents the application of a 3D point cloud in the presentation of Bloodstain Pattern Analysis evidence to mock jurors. 150 mock jurors viewed a presentation of Bloodstain Pattern Analysis evidence from a murder trial in the UK.

Soil quality index (SQI) for evaluating the sustainability status of Kakia-Esamburmbur catchment under three different land use types in Narok County, Kenya.

Land and water degradation caused by soil erosion and climate change pose major environmental threats, particularly in agricultural watersheds. Soil erosion in a catchment leads to low crop yields due to declining soil quality (SQ), productivity and sustainability. However, very few studies have been done to assess soil health in Kenya, and none in Narok County.

Performance and emission characteristics of a diesel engine fuelled by biodiesel from black soldier fly larvae: Effects of synthesizing catalysts with citric acid.

Biodiesel has several environmental benefits, such as biodegradability, renewability and lower soot emissions. However, biodiesel has undesirable properties such as higher viscosity and density and low calorific value compared to petroleum diesel, resulting in high Brake Specific Fuel Consumption (BSFC), reduced Brake Power (BP) and increased NO emissions creating an environmental concerns in biodiesel development. This study investigated the effects of synthesizing transesterification catalysts (CaO and NaOH) with Citric Acid (CA) on the quality of biodiesel and biodiesel blends produced from Black Soldier Fly Larvae (BSFL) (Hermetia ).

Insulin biobetters and biosimilars in clinical practice.

Insulin injections have never been an entirely satisfactory therapy, and as a result a continuing 'biobetter' technological cascade has driven changes in purity and manufacture, in structure and excipients, and in administration devices. The resulting deck of insulin preparations has to be matched by health-care teams and users with individual need. This latter is itself a complex ranging from ambulatory care in type 1 and type 2 diabetes, the topic generally addressed by guidelines and funding advice, to in-patient care and the newly diagnosed, plus secondary diabetes with very different effects on insulin need, through to co-morbidities and medications interfering with glucose metabolism.

Findings for iGlarLixi versus BIAsp 30 confirmed in groups of people with type 2 diabetes with different biomedical characteristics.

Aim: To report prespecified and post hoc analyses of the SoliMix dataset exploring the impact of baseline participant characteristics on the original SoliMix study outcomes, to enable informed treatment choices for people with different biomedical characteristics.

Methods: SoliMix (EudraCT 2017-003370-13) compared once-daily iGlarLixi (a fixed-ratio combination of insulin glargine 100 U/mL and the glucagon-like peptide-1 receptor agonist lixisenatide) with twice-daily BIAsp 30 (30% insulin aspart and 70% insulin aspart protamine). In this analysis, the original primary outcomes of noninferiority of iGlarLixi versus BIAsp 30 in terms of glycated haemoglobin (HbA1c) change and superiority in terms of body weight change, together with change in basal insulin dose and hypoglycaemia outcomes, were investigated by baseline age, duration of diabetes, insulin dose, HbA1c level, body mass index (BMI), and renal function.

Hypoglycaemia events with iGlarLixi versus premix biphasic insulin aspart 30 (BIAsp 30) in people with type 2 diabetes advancing from basal insulin: An analysis of the SoliMix trial.

Aims: To explore details of the incidence and rates of daytime and nocturnal hypoglycaemia, levels of hypoglycaemia, and relationship to glycated haemoglobin (HbA1c), when comparing iGlarLixi versus premixed biphasic insulin aspart 30 (BIAsp 30) in the SoliMix randomized controlled trial.

Materials And Methods: This exploratory analysis of SoliMix used logistic regression and negative binomial regression analyses to assess between-treatment differences in the incidence and rates of hypoglycaemia by time of day. A negative binomial model was used to derive estimated annualized hypoglycaemia rates as a function of HbA1c.

Heating and emission characteristics from combustion of charcoal and co-combustion of charcoal with faecal char-sawdust char briquettes in a ceramic cook stove.

Over reliance on charcoal has accelerated deforestation in sub-Saharan Africa. Seeking alternative sustainable and environmentally friendly sources of biomass energy to meet the escalating energy demand is therefore vital. However, limited evidence exists on the concentrations of toxic emissions of different biomass fuels.

Quantifying forensic investigations involving bloodstain pattern analysis within the UK.

Research informed by practice is more likely to have a greater impact on society. However, forensic cases are confidential and thus, real-life data regarding the details of violent crime is usually withheld from the public and academia. Through a partnership between the authors institution and a UK police service, casefiles from 78 criminal investigations from 2012 to 2020 involving Bloodstain Pattern Analysis (BPA) were examined and quantified.

Evaluation of water quality and potential scaling of corrosion in the water supply using water quality and stability indices: A case study of Juja water distribution network, Kenya.

Drinking water quality describes the conditions for water to be accepted as suitable for human consumption. The water quality index is characterized by including, in the assessment process, water quality parameters such as physical, chemical and micro-biological. The availability of adequate management strategies to maintain good quality water has always been a challenge for water utilities.

Understanding Biosimilar Insulins - Development, Manufacturing, and Clinical Trials.

Background: A wave of expiring patents for first-generation insulin analogues has created opportunities in the global insulin market for highly similar versions of these products, biosimilar insulins. Biologics are generally large, complex molecules produced through biotechnology in a living system, such as a microorganism, plant cell, or animal cell. Since manufacturing processes of biologics vary, biosimilars cannot be exact copies of their reference product but must exhibit a high degree of functional and structural similarity.

Software for the trajectory analysis of blood-drops: A systematic review.

Blood-drop trajectory analysis can provide investigators with retrospective information regarding the spatial positioning of an injured person. To assist with bloodstain pattern analysis, various commercially available software have been developed and deployed. A systematic review was conducted to understand the extent of experimental validation and applications of blood-drop trajectory analysis software to case work.

Future directions in insulin therapy.

Insulin therapy has a long history at the cutting edge of technological development through purification, extended-action, molecular chemistry, and devices, and in support technologies including self-measurement and patient education. But unmet needs remain large. Today's therapy cannot deliver minute-to-minute control of glucose levels, and cannot imitate the reflex/incretin driven physiological insulin delivery at mealtimes.

Insulin therapy development beyond 100 years.

The first insulin preparation capable of consistently lowering blood glucose was developed in 1921. But 100 years later, blood glucose control with insulin in people with diabetes is nearly universally suboptimal, with essentially the same molecule still delivered by the same inappropriate subcutaneous injection route. Bypassing this route with oral administration appears to have become technologically feasible, accelerating over the past 50 years, either with packaged insulin peptides or by chemical insulin mimetics.

Efficacy and safety of iGlarLixi versus IDegAsp: Results of a systematic literature review and indirect treatment comparison.

Aim: To assess the efficacy and safety of iGlarLixi, a fixed-ratio combination of basal insulin glargine 100 U/mL and lixisenatide (glucagon-like peptide-1 receptor agonist) versus IDegAsp, a co-formulation of basal insulin degludec 100 U/mL with rapid-acting insulin aspart.

Materials And Methods: A systematic literature search of randomized controlled trials (RCTs) was performed. Outcomes from eligible RCTs were compared by an indirect treatment comparison using a Bayesian framework.

Pineapple peel biochar and lateritic soil as adsorbents for recovery of ammonium nitrogen from human urine.

Human urine is a rich source of nitrogen which can be captured to supplement the existing sources of nitrogen fertilizers thus contributing to enhanced crop production. However, urine is the major contributor of macronutrients in municipal wastewater flows resulting into eutrophication of the receiving water bodies. Herein, pineapple peel biochar (PPB), and lateritic soil (LS) adsorbents were prepared for the safe removal of ammonium nitrogen (NH-N) from human urine solutions.

Heart failure management; a perspective from diabetes care.

People with type 2 diabetes (T2DM) are recognized as having a 2-4 times increased risk of heart failure (HF). Ambulatory diabetes care has long concentrated on the prevention of microvascular and arterial disease, and surveillance for manageable problems such as with the feet and retinae. Accordingly, management of heart failure has never been a specific focus, although the preventative management of cardiac and kidney disease through glucose-lowering, blood pressure (BP) control, and blood lipid control, have had a positive impact on its incidence.

The evolution of insulin therapy.

Evolution in medicine is generally driven by clinical need, hand in hand with opportunities generated by novel chemical and mechanical engineering technologies. Since 1921 that has been a continuing paradigm for insulin therapy, some advances being a continual process, and others arising from external scientific or engineering developments. Purification of insulin preparations was an early issue, resolved in the 1970s, then challenged by the switch to manufacture in microorganisms.

Occurrence, distribution, and risk assessment of pharmerciuticals in wastewater and open surface drains of peri-urban areas: Case study of Juja town, Kenya.

The occurrence of Active Pharmaceutical Ingredients (APIs) in the environment is becoming a major area of concern due to their undesirable effects on non-target organisms. This study investigated the occurrence and risk of contamination by five antibiotics and three antiretrovirals drugs in a fast-growing peri-urban area in Kenya, with inadequate sewer system coverage. Due to poor sewage connectivity and poorly designed decentralized systems, wastewater is directly released in open drains.

Insulin glargine/lixisenatide fixed-ratio combination (iGlarLixi) compared with premix or addition of meal-time insulin to basal insulin in people with type 2 diabetes: A systematic review and Bayesian network meta-analysis.

Aim: To assess the efficacy and safety of iGlarLixi, a fixed-ratio combination of insulin glargine 100 U/mL and lixisenatide, relative to premix insulin and other insulin options through network meta-analysis.

Methods: A systematic literature search identified randomized controlled trials (RCTs) comparing iGlarLixi, premix insulin or basal insulin (BI) in combination with meal-time insulin, in people inadequately controlled with BI. Eligible RCTs were compared using Bayesian network meta-analysis.

Impact of a Weekly Glucagon-Like Peptide 1 Receptor Agonist, Albiglutide, on Glycemic Control and on Reducing Prandial Insulin Use in Type 2 Diabetes Inadequately Controlled on Multiple Insulin Therapy: A Randomized Trial.

Objective: The principle of replacing prandial insulin lispro with a once-weekly glucagon-like peptide 1 receptor agonist (GLP-1RA) for type 2 diabetes inadequately controlled on a multiple daily insulin injections regimen was tested with albiglutide.

Research Design And Methods: In this treat-to-target study, basal plus prandial insulin was optimized over 4 weeks before participants were randomized (1:1) to albiglutide plus optimized basal insulin glargine and lispro (dose reduced by 50% at randomization; subsequently, lispro injections were fully discontinued 4 weeks later) ( = 402) or to continued optimized lispro plus optimized glargine ( = 412).

Results: Mean ± SD HbA at baseline, 7.